Plasmid
pYES2 His YPL191c
Parent Plasmid
pYES
Expressed
Gal promoter- 6His-YPL191c
Genbank
NM_001184005.1.
Sequence of Insert
View Sequence
GGATCCATGCATCATCACCATCACCATGATTTAAGTTTCACGACTAAAAGTGTCAAAATTAATGGTCAAAACCATCGAATATTACTGCAAAACGAAAACGGACCTTGTGCCTTGTTAGCACTTGCAAATATTCTAATACTTTCACCAGATCATACACGTTTTTCCAATGAGTTAATAAGGTTGGTAAATAAGGGAAGTCAAATATCTTTAAAAGAACTTATTGAAGTCTTAGCAGATATCGCTTTACAAGTTACAGATAAACCTAGTACTGATATAAGTGAGTTACTAAGTTTGTTACCTAGATTACATGAAGGTTTAAATATTAACCCTGAGTTTAATGGGTCTTTTGAAAATACTAAAGAGATGTCAATATTTCGATTGTTTAACGTTGATGTTGTCCATGGCTGGGTTATCAACAGTTTCATAAATGAAAACATTGATGAGAAACTATCTCATTATTCTTATGAATCTGCGCAGAGAATCTTAACACAAGCAGCTGATATAAATTGTGGAATTTCCCAAGATGAAAACTCGGACGAAGTTTTACGTGATGCCATGCATTTGGGTCTGTTTTTGAACGAATCACCGACTCAGTTGACCGCCTTTGGACTCCTACGATTGAGAGAAAAGCTGCTACACAACAAATTCTCCATTTTATTCAGAAATGACCATTTTTCTACATTGTTCAAATACGAAGATCGATTATACACATTGGTTACGGACTTTGGTTATAAAAATTGTAAAGATATTGTATGGCAATCCCTAGATTCGGTGGACGGCTCCTGCGATGCTTTTTTTGCCGGTAACTTTAGTGCAGCAGAGGTAAATGGACAGCAATTGTCAACTGACATTGAACGTGATTTTGGAACTGGAAATTTGCTACTTGAAGAAATTCAGCAAATTGAAAACGATAAAGAACTAGCCAAACAGTTACAGGAACAAGAACAAGAACGTGTTACGAAATTTGAAGCAAAAAGGAAAATCCATTCTCACAAAAAAAACTCAGAAATACACGCACCAGTCAAAAAAGACAAGTTTAAGCGAAGAAGCAGCCTTCTCAATGCAAAAGCTTCTGAAAAAGAGAAAAGTGAGTGCGTTGTTATGTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHDLSFTTKSVKINGQNHRILLQNENGPCALLALANILILSPDHTRFSNELIRLVNKGSQISLKELIEVLADIALQVTDKPSTDISELLSLLPRLHEGLNINPEFNGSFENTKEMSIFRLFNVDVVHGWVINSFINENIDEKLSHYSYESAQRILTQAADINCGISQDENSDEVLRDAMHLGLFLNESPTQLTAFGLLRLREKLLHNKFSILFRNDHFSTLFKYEDRLYTLVTDFGYKNCKDIVWQSLDSVDGSCDAFFAGNFSAAEVNGQQLSTDIERDFGTGNLLLEEIQQIENDKELAKQLQEQEQERVTKFEAKRKIHSHKKNSEIHAPVKKDKFKRRSSLLNAKASEKEKSECVVM*
Vector Type
Yeast
DU Number
DU55483
Unit Source
Location
PPU
MTA
Notes
Hi,
Could you clone the YPL191c gene under the Gal promoter, with a 6His tag at the N-terminal of YPL191c. The selection marker can be any auxotrophy (leu, ura, his, trp or ade), or antibiotic except for hygromycin.
Thank you
Marjorie
Could you clone the YPL191c gene under the Gal promoter, with a 6His tag at the N-terminal of YPL191c. The selection marker can be any auxotrophy (leu, ura, his, trp or ade), or antibiotic except for hygromycin.
Thank you
Marjorie
Low Temp
No
Price
£125.00
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