cDNA Clone YGL082w

• Plasmid: pYES2 His YGL082W
• Parent Plasmid: pYES2
• Expressed: Gal promoter-6His-YGL082w
• Genbank: NM_001180947.3
• Tag: 6His

Sequence of Insert

GAATTCATGCATCATCACCATCACCATGATGTTACATTTCTAACCAAAAATGTTCAAATAAATGGCACTCAGTTTAAAATATTACTTCAAAATGGGCAAGGTGAATGCGCGCTAATTGCACTAGCTAACGTATTATTGATATCACCTGCCCATGCTCGCTATGCCCAAGAAATTTCCAGACTAGTGAGAGGCAAGGAAACTGTTACTTTAAACGAACTTGTGCAAACTCTAGCCGACATGGGCGTTCAAAATCCGAATGGTACAGATGTGGACAAACAACAGCTACTCCAAATCCTACCACAATTATATAGCGGATTGAACATCAACCCAGAATTTAACGGATCTTTTGAGGACGGCGTAGAAATGTCGATTTTCAGGCTCTACAATGTCGGCATCGTTCATGGATGGATAATAGATGGGGACAATGATCCGAACTCTTACGAACATGTGTCCAAATATTCTTATATGGGCGCGCAAAAAGTTTTGGTACAGTCGTATGAGATCCAAAAGAATAATGCCCAATTTGAAAACAGCGAGCAAATCCAATCTGATGCACCTTACTTAAAATCGTTTCTGGCAAGATCTGCCACACAATTGACTGAATATGGTCTAACTCATCTCAGAGAAATTTTAGTAGAAAGATCGTATGCCGTGCTTTTTAGAAATGATCACTTTTGCACATTATATAAAAACAATGGCGAATTGTTTACTTTGGTCACGGATCCTACTTACAGAAATCGCAAGGACATAAACTGGCAGTCGCTTAAATCCGTGAATGGATCGCAGGATTCTTACTACACGGGCAACTTCATTCCCACAAGTCTGGAAAGAACAGAAACTACTGCTACCGGGCAAAATGAGTCCTACATTTCTAACCCATTCAGTGACCAGAATACAGGGCATGTAACTAGCAACCAAGTTAATAGTGGAGCAAGCGGTGTTCAACAAATCGAAGATGATGAGGAACTAGCTAGGAGGTTACAGGAACAAGAAGATATGCGTGCGGCAAATAACATGCAAAATGGTTATGCTAATAACGGTAGAAATCATCAAAGGGAAAGGTTTGAAAGACCTGAAAAAAATTCAAAGAAAAACAAGTTCCTTCCCTTTAATGGGAGTAATAAAGAAAAGAAAAGAGACAAGTTGAAAAAAAACTGTGTAATTATGTGAGCGGCCGC

Amino Acid Sequence

MHHHHHHDVTFLTKNVQINGTQFKILLQNGQGECALIALANVLLISPAHARYAQEISRLVRGKETVTLNELVQTLADMGVQNPNGTDVDKQQLLQILPQLYSGLNINPEFNGSFEDGVEMSIFRLYNVGIVHGWIIDGDNDPNSYEHVSKYSYMGAQKVLVQSYEIQKNNAQFENSEQIQSDAPYLKSFLARSATQLTEYGLTHLREILVERSYAVLFRNDHFCTLYKNNGELFTLVTDPTYRNRKDINWQSLKSVNGSQDSYYTGNFIPTSLERTETTATGQNESYISNPFSDQNTGHVTSNQVNSGASGVQQIEDDEELARRLQEQEDMRAANNMQNGYANNGRNHQRERFERPEKNSKKNKFLPFNGSNKEKKRDKLKKNCVIM*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU55456
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Hi,; Could you clone the YGL082w gene under the GAL promoter, with a 6His tag at the N terminal. You can use any selection marker you wish except Hygromycin.; Thanks,; Marjorie
• Low Temp: No