cDNA Clone VHL

• Synonyms: HRCA1, RCA11, pVHL, VHL
• Plasmid: pBABED P FLAG VHL no stop
• Parent Plasmid: pBabeD puro FLAG
• Expressed: FLAG-VHL-5xGLY
• Genbank: NM_000551.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGTCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATGGTGGAGGCGGAGGTGAATTC

Amino Acid Sequence

MDYKDDDDKGSAMPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVW
LNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDL
EDHPNVQKDLERLTQERIAHQRMGDGGGGGEF

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBABED P FLAG VHL no stop77.2 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU54795
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: For addition of alternative mono and nanobodies in degron system development (Eco/Sal/Xho/Not for insertion).
• Low Temp: No