cDNA Clone alpha-Tubulin

• Synonyms: TUBA1A
• Plasmid: pSc-B-TUBA1A
• Parent Plasmid: pSC-B
• Genbank: 7846
• Species: Human

Sequence of Insert

GGATCCATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCA
GATTGGCAATGCCTGCTGGGAGCTCTACTGCCTGGAACACGGCATCCAGC
CCGATGGCCAGATGCCAAGTGACAAGACCATTGGGGGAGGAGATGACTCC
TTCAACACCTTCTTCAGTGAGACGGGCGCTGGCAAGCACGTGCCCCGGGC
TGTGTTTGTAGACTTGGAACCCACAGTCATTGATGAAGTTCGCACTGGCA
CCTACCGCCAGCTCTTCCACCCTGAGCAGCTCATCACAGGCAAGGAAGAT
GCTGCCAATAACTATGCCCGAGGGCACTACACCATTGGCAAGGAGATCAT
TGACCTTGTGTTGGACCGAATTCGCAAGCTGGCTGACCAGTGCACCGGTC
TTCAGGGCTTCTTGGTTTTCCACAGCTTTGGTGGGGGAACTGGTTCTGGG
TTCACCTCCCTGCTCATGGAACGTCTCTCAGTTGATTATGGCAAGAAATC
CAAGCTGGAGTTCTCCATTTACCCGGCACCCCAGGTTTCCACAGCTGTAG
TTGAGCCCTACAACTCCATCCTCACCACCCACACCACCCTGGAGCACTCT
GATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGACATCTGTCGTAG
AAACCTCGATATCGAGCGCCCAACCTACACTAACCTTAACCGCCTTATTA
GCCAGATTGTGTCCTCCATCACTGCTTCCCTGAGATTTGATGGAGCCCTG
AATGTTGACCTGACAGAATTCCAGACCAACCTGGTGCCCTACCCCCGCAT
CCACTTCCCTCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAAAGCCT
ACCATGAACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCTTTGAGCCA
GCCAACCAGATGGTGAAATGTGACCCTCGCCATGGTAAATACATGGCTTG
CTGCCTGTTGTACCGTGGTGACGTGGTTCCCAAAGATGTCAATGCTGCCA
TTGCCACCATCAAAACCAAGCGCAGCATCCAGTTTGTGGATTGGTGCCCC
ACTGGCTTCAAGGTTGGCATCAACTACCAGCCTCCCACTGTGGTGCCTGG
TGGAGACCTGGCCAAGGTACAGAGAGCTGTGTGCATGCTGAGCAACACCA
CAGCCATTGCTGAGGCCTGGGCTCGCCTGGACCACAAGTTTGACCTGATG
TATGCCAAGCGTGCCTTTGTTCACTGGTACGTGGGTGAGGGGATGGAGGA
AGGCGAGTTTTCAGAGGCCCGTGAAGATATGGCTGCCCTTGAGAAGGATT
ATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGAGGGTGAGGAAGAAGGA
GAGGAATACTAAGCGGCCGC

Amino Acid Sequence

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFN
TFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAA
NNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFT
SLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDC
AFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNV
DLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTG
FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYA
KRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEE
Y*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU28554
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone contains the following silent change compared to NCBI ref shown:; D47 (GAT > GAC), G57 (GGG > GGC), H61 (CAT > CAC), A65 (GCA > GCT), Q91 (CAA > CAG), L92 (CTT > CTC), K96 (AAA > AAG), L117 (CTC > CTT), A126 (GCC > GCT), T130 (ACG > ACC), L132 (CTC > CTT), S151 (TCG > TCC), K164 (AAG > AAA), S170 (TCT > TCC), A174 (GCG > GCA), I219 (ATT > ATC), R221 (CGT > CGC), Y224 (TAT > TAC), L227 (CTG > CTT), N228 (AAT > AAC), R229 (AGG > CGC), L230 (TTA > CTT), I231 (ATA > ATT), Q233 (CAA > CAG), Y262 (TAT > TAC), K336 (AAG > AAA), R339 (CGT > CGC), K401 (AAA > AAG), V409 (GTT > GTG), G410 (GGG > GGT), G416 (GGT > GGC), E423 (GAG > GAA), D424 (GAC > GAT),; ; Also two non-silent mutations to correct *** Gly232Ser and Thr340Ser ***