cDNA Clone ATG101

• Plasmid: pBabeD puro HA BirA R118G ATG101
• Parent Plasmid: pBabeD puro HA BirA R118G
• Expressed: HA-BirA R118G-ATG101
• Genbank: NM_021934.4 GI:149158712
• Tag: HA
• Tag: BirA

Sequence of Insert

GGATCCATGAACTGTCGCTCGGAGGTGCTGGAGGTGTCGGTGGAGGGGCGGCAGGTGGAGGAGGCCATGCTGGCTGTGCTGCACACGGTGCTTCTGCACCGCAGCACAGGCAAGTTCCACTACAAGAAGGAGGGCACCTACTCCATTGGCACCGTGGGCACCCAGGATGTTGACTGTGACTTCATCGACTTCACTTATGTGCGTGTCTCTTCTGAGGAACTGGATCGTGCCCTGCGCAAGGTTGTTGGGGAGTTCAAGGATGCACTGCGCAACTCTGGTGGCGATGGGCTGGGGCAGATGTCCTTGGAGTTCTACCAGAAGAAGAAGTCTCGCTGGCCATTCTCAGACGAGTGCATCCCATGGGAAGTGTGGACGGTCAAGGTGCATGTGGTAGCCCTGGCCACGGAGCAGGAGCGGCAGATCTGCCGGGAGAAGGTGGGTGAGAAACTCTGCGAGAAGATCATCAACATCGTGGAGGTGATGAATCGGCATGAGTACTTGCCCAAGATGCCCACACAGTCGGAGGTGGATAACGTGTTTGACACAGGCTTGCGGGACGTGCAGCCCTACCTGTACAAGATCTCCTTCCAGATCACTGATGCCCTGGGCACCTCAGTCACCACCACCATGCGCAGGCTCATCAAAGACACCCTTGCCCTCTGAGAATTC

Amino Acid Sequence

MYPYDVPDYAGGMKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEKGSMNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWEVWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDTGLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55321
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please subclone ATG101 into pbabe with N-terminal HA-BirA R118G (similar to DU47686)