cDNA Clone CEP131

• Synonyms: AZ1; AZI1; ZA1
• Plasmid: pcDNA5D FRT/TO mCherry CEP131
• Parent Plasmid: pcDNA5 FRT/TO mCherry
• Expressed: mCherry CEP131
• Genbank: NM_001319228.1 GI:984880705*
• Tag: mCherry
• Species: Human

Sequence of Insert

GGATCCATGAAAGGCACCCGGGCCATCGGCAGCGTCCCGGAGCGCAGCCCAGCAGGTGTGGACCTGAGTCTGACAGGTCTCCCTCCGCCTGTGTCCCGGCGTCCTGGCAGTGCCGCCACCACCAAGCCCATCGTCCGCTCTGTCTCCGTGGTCACAGGCAGCGAGCAGAAGAGGAAGGTGCTGGAGGCCACAGGGCCTGGGGGCTCCCAGGCCATCAACAACCTTAGAAGATCCAACAGCACCACGCAGGTCAGCCAGCCTCGGAGCGGCTCCCCCAGGCCAACGGAGCCCACAGACTTCCTGATGCTCTTCGAGGGCAGCCCCAGTGGGAAAAAGAGGCCTGCCAGCCTGAGCACAGCCCCCAGCGAGAAGGGAGCCACCTGGAACGTCCTGGATGACCAGCCCCGGGGCTTCACCTTGCCATCCAATGCCCGGAGTTCCAGTGCCCTTGACTCACCAGCGGGCCCGCGGAGGAAAGAATGCACCGTGGCCCTGGCCCCCAACTTCACTGCTAACAACAGGAGCAACAAGGGAGCAGTGGGCAACTGCGTCACCACCATGGTGCACAACCGCTACACCCCCTCGGAGAGGGCGCCTCCGCTCAAGAGCTCCAACCAGACTGCCCCCTCCCTCAACAACATCATCAAGGCAGCCACCTGTGAGGGCAGTGAGAGCAGCGGCTTTGGGAAGCTGCCGAAGAATGTCTCCAGTGCCACCCACTCAGCCCGGAACAATACTGGGGGCAGCACGGGCTTGCCCAGGCGGAAGGAGGTGACGGAGGAGGAGGCTGAGAGGTTTATCCACCAGGTGAACCAGGCCGCTGTCACCATCCAGCGCTGGTACCGGCACCAGGTGCAGCGGCGCGGAGCAGGAGCTGCCCGCCTGGAGCACTTGCTTCAGGCCAAGCGGGAGGAGCAGCGGCAGCGGTCAGGCGAGGGGACCCTCTTGGACCTGCACCAGCAGAAAGAGGCAGCCAGGAGGAAGGCCCGGGAGGAGAAGGCACGCCAAGCCAGGCGAGCAGCCATTCAGGAGCTGCAACAGAAACGAGCCCTGAGAGCCCAGAAGGCGAGCACTGCCGAGCGTGGGCCACCTGAGAATCCCAGGGAGACCAGAGTGCCAGGAATGCGGCAGCCTGCTCAGGAGCTGTCCCCCACGCCAGGCGGCACTGCCCACCAGGCCCTCAAGGCCAACAATGCTGGTGGTGGCCTCCCTGCTGCAGGCCCCGGAGACCGCTGCCTGCCCACCTCCGACTCATCCCCAGAACCACAGCAGCCTCCAGAGGACAGGACGCAGGACGTTCTTGCCCAGGATGCAGCTGGGGACAACCTGGAGATGATGGCCCCGAGCAGGGGGAGCGCCAAGTCCAGGGGGCCACTGGAGGAGCTGCTGCACACACTGCAGCTGCTGGAGAAGGAGCCGGACGCGCTGCCCCGCCCCAGGACCCATCACAGGGGCAGATACGCCTGGGCCAGCGAGGTGACCACGGAGGATGACGCCAGCTCTCTGACAGCTGACAACTTGGAGAAATTTGGAAAACTCAGTGCGTTCCCCGAACCTCCTGAGGATGGGACGCTGCTATCGGAGGCCAAGCTACAAAGCATCATGAGCTTCTTGGACGAGATGGAGAAGTCTGGGCAGGACCAGCTGGACTCCCAGCAGGAGGGGTGGGTGCCGGAGGCGGGGCCGGGGCCCCTGGAGCTGGGGTCCGAGGTGAGCACGTCTGTGATGCGGCTGAAGCTGGAGGTGGAGGAGAAGAAGCAGGCCATGCTGCTGCTGCAGAGAGCGCTGGCGCAGCAGCGAGACCTCACGGCCCGGCGGGTCAAGGAGACAGAGAAGGCACTGAGCCGGCAGCTGCAGCGGCAGAGGGAGCACTACGAGGCCACCATCCAGCGGCACTTGGCCTTCATTGACCAGCTGATTGAGGACAAGAAGGTCCTGAGTGAAAAGTGCGAGGCTGTGGTGGCCGAGCTGAAGCAGGAGGACCAGAGATGCACCGAGCGTGTGGCCCAGGCACAGGCGCAGCACGAGCTGGAGATTAAAAAACTCAAAGAATTAATGAGCGCCACCGAGAAAGCCCGCCGGGAGAAGTGGATCAGTGAGAAAACCAAGAAGATCAAGGAGGTCACTGTCCGAGGTCTGGAGCCCGAGATCCAGAAGCTGATTGCAAGGCACAAGCAGGAAGTGCGGAGGCTCAAGAGCCTGCACGAGGCGGAGCTGCTGCAGTCGGATGAGCGGGCCTCGCAGCGCTGCCTGCGCCAGGCCGAGGAGCTGCGGGAGCAGCTGGAGCGGGAGAAGGAGGCGCTGGGCCAGCAGGAGCGCGAACGTGCTCGGCAGCGGTTCCAGCAGCACCTGGAGCAGGAGCAGTGGGCGCTGCAGCAGCAACGGCAGCGGCTGTACAGTGAGGTGGCTGAGGAGAGGGAGCGGCTGGGCCAGCAGGCAGCCAGGCAGCGGGCGGAGCTGGAAGAGCTGAGGCAGCAGCTGGAGGAGAGCAGCTCTGCACTGACCCGAGCCCTGAGGGCTGAGTTTGAGAAGGGCAGGGAGGAGCAGGAGCGCCGGCACCAGATGGAGCTGAATACCCTGAAGCAGCAGCTGGAGCTGGAGAGGCAGGCGTGGGAGGCCGGCCGCACCAGGAAGGAGGAGGCGTGGCTGCTGAACCGGGAACAGGAGCTGAGGGAAGAAATCCGGAAAGGCCGGGACAAGGAGATTGAGCTGGTCATTCACCGGCTGGAGGCCGACATGGCGCTGGCCAAGGAGGAGAGTGAGAAGGCTGCCGAGAGCCGCATCAAGCGCTTACGGGACAAGTACGAGGCCGAGCTCTCCGAGCTGGAGCAGTCGGAGCGGAAGCTTCAGGAGCGGTGCTCGGAGCTGAAGGGCCAGCTTGGGGAGGCCGAGGGCGAGAATCTGCGTCTGCAGGGCCTTGTGCGGCAGAAGGAGCGGGCGCTGGAGGATGCGCAGGCGGTGAACGAGCAGCTTTCTAGCGAGCGCAGCAACCTGGCCCAGGTGATCCGCCAGGAGTTCGAGGACCGGCTGGCAGCCTCTGAGGAGGAGACGCGGCAGGCCAAGGCCGAGCTGGCCACGCTGCAGGCCCGCCAGCAGCTGGAGCTGGAGGAGGTGCACCGGAGGGTGAAGACAGCCCTCGCGAGGAAGGAGGAGGCCGTGAGCAGCCTCCGGACACAACATGAGGCTGCGGTGAAGCGGGCCGACCACCTGGAGGAGCTGCTGGAGCAGCACAGGAGGCCCACGCCAAGTACCAAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMKGTRAIGSVPERSPAGVDLSLTGLPPPVSRRPGSAATTKPIVRSVSVVTGSEQKRKVL
EATGPGGSQAINNLRRSNSTTQVSQPRSGSPRPTEPTDFLMLFEGSPSGKKRPASLSTAPSEKGATWNVLDDQPRGFTLPSNARSSSALDSPAGPRRKEC
TVALAPNFTANNRSNKGAVGNCVTTMVHNRYTPSERAPPLKSSNQTAPSLNNIIKAATCEGSESSGFGKLPKNVSSATHSARNNTGGSTGLPRRKEVTEE
EAERFIHQVNQAAVTIQRWYRHQVQRRGAGAARLEHLLQAKREEQRQRSGEGTLLDLHQQKEAARRKAREEKARQARRAAIQELQQKRALRAQKASTAER
GPPENPRETRVPGMRQPAQELSPTPGGTAHQALKANNAGGGLPAAGPGDRCLPTSDSSPEPQQPPEDRTQDVLAQDAAGDNLEMMAPSRGSAKSRGPLEE
LLHTLQLLEKEPDALPRPRTHHRGRYAWASEVTTEDDASSLTADNLEKFGKLSAFPEPPEDGTLLSEAKLQSIMSFLDEMEKSGQDQLDSQQEGWVPEAG
PGPLELGSEVSTSVMRLKLEVEEKKQAMLLLQRALAQQRDLTARRVKETEKALSRQLQRQREHYEATIQRHLAFIDQLIEDKKVLSEKCEAVVAELKQED
QRCTERVAQAQAQHELEIKKLKELMSATEKARREKWISEKTKKIKEVTVRGLEPEIQKLIARHKQEVRRLKSLHEAELLQSDERASQRCLRQAEELREQL
EREKEALGQQERERARQRFQQHLEQEQWALQQQRQRLYSEVAEERERLGQQAARQRAELEELRQQLEESSSALTRALRAEFEKGREEQERRHQMELNTLK
QQLELERQAWEAGRTRKEEAWLLNREQELREEIRKGRDKEIELVIHRLEADMALAKEESEKAAESRIKRLRDKYEAELSELEQSERKLQERCSELKGQLG
EAEGENLRLQGLVRQKERALEDAQAVNEQLSSERSNLAQVIRQEFEDRLAASEEETRQAKAELATLQARQQLELEEVHRRVKTALARKEEAVSSLRTQHE
AAVKRADHLEELLEQHRRPTPSTK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU53444
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Three residue changes compared with NM_001319228.1 CDS:; T272A, T397A, V473A; (The cloned sequence matches EAW89632.1. Changes are RNA-derived and also seen in EST sequences. Also, no tryptic peptides were identified around these residues by Mass-spec, although there are R/K residues that should have given peptides. This suggests that what was obtained from RNA sources could very well be the dominant isoform in cells.)