cDNA Clone UFC1

• Synonyms: -
• Plasmid: pET15 6His 3C UFC1 Y90C
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C UFC1 Y90C
• Genbank: NM_016406.3 GI:156415999*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGATGAAGCCACGCGACGTGTTGTGTCTGAGATCCCGGT
GCTGAAGACTAACGCCGGACCCCGAGATCGTGAGTTGTGGGTGCAGCGAC
TGAAGGAGGAATATCAGTCCCTTATCCGGTATGTGGAGAACAACAAGAAT
GCTGACAACGATTGGTTCCGACTGGAGTCCAACAAGGAAGGAACTCGGTG
GTTTGGAAAATGCTGGTATATCCATGACCTCCTGAAATATGAGTTTGACA
TCGAGTTTGACATTCCTATCACATGTCCTACTACTGCCCCAGAAATTGCA
GTTCCTGAGCTGGATGGAAAGACAGCAAAGATGTACAGGGGTGGCAAAAT
ATGCCTGACGGATCATTTCAAACCTTTGTGGGCCAGGAATGTGCCCAAAT
TTGGACTAGCTCATCTCATGGCTCTGGGGCTGGGTCCATGGCTGGCAGTG
GAAATCCCTGATCTGATTCAGAAGGGCGTCATCCAACACAAAGAGAAATG
CAACCAATGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNADNDWFRLESNKEGTRWFGKCWYIHDLLK
YEFDIEFDIPITCPTTAPEIAVPELDGKTAKMYRGGKICLTDHFKPLWARNVPKFGLAHLMALGLGPWLAVEIPDLIQKGVIQHKEKCNQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15 6His 3C UFC1 Y90C58.29 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU53290
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_016406.3 CDS: a269g (Y90C) (matches BC005187.1 GI:13528770) - renders protein insoluble; Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 46221 Daltons
• Low Temp: No