cDNA Clone Brd2 (ms)

• Plasmid: pCAGGS 3xFLAG ms Brd2
• Parent Plasmid: pCAGGS 3xFLAG
• Expressed: FLAG-mouse Brd2
• Tag: 3FLAG

Sequence of Insert

GAATCCATGCTGCAAAACGTGACTCCCCACAAGCTCCCTGGGGAAGGGAATGCAGGGTTATTGGGGCTGGGCCCAGAGGCAGCAGCACCAGGGAAAAGGATTCGAAAGCCTTCTCTGCTGTATGAGGGATTTGAGAGCCCCACAATGGCTTCTGTACCAGCTTTACAACTGGCCCCTGCCAATCCACCACCCCCTGAGGTGTCCAATCCCAAAAAGCCAGGACGGGTAACAAACCAACTGCAGTACCTGCACAAGGTAGTGATGAAGGCTCTGTGGAAGCATCAGTTTGCATGGCCATTCCGGCAGCCTGTGGACGCTGTGAAGCTGGGTTTGCCGGATTATCACAAAATTATAAAACAGCCTATGGACATGGGTACTATCAAGAGGAGACTTGAAAACAATTACTACTGGGCTGCCTCAGAATGTATGCAGGATTTTAATACTATGTTTACCAACTGTTACATTTATAACAAGCCCACCGATGATATTGTCCTAATGGCACAGACACTGGAAAAGATCTTCCTACAGAAAGTGGCATCCATGCCACAAGAGGAGCAAGAGCTTGTGGTGACCATCCCTAAAAACAGCCATAAGAAGGGGGCCAAGTTAGCAGCACTCCAGGGCAGTATTACCAGTGCCCATCAGGTGCCTGCTGTCTCTTCTGTGTCGCATACAGCCCTGTATACACCACCACCTGAAATACCTACCACTGTCCTCAACATTCCCCACCCATCAGTCATCTCTTCTCCTCTTCTTAAGTCCCTGCATTCTGCTGGACCCCCACTCCTTGCTGTATCAGCAGCGCCTCCAGCTCAGCCCCTTGCCAAGAAAAAAGGCGTTAAACGGAAAGCGGATACTACCACCCCTACACCCACAGCCATCCTGGCTCCTGGTTCCCCAGCTAGTCCTCCTGGGAGTCTTGAGCCAAAGGCAGCAAGGCTCCCTCCTATGCGCAGAGAGAGTGGCCGCCCAATCAAACCCCCACGAAAAGACTTGCCTGACTCGCAACAGCAACACCAGAGCTCTAAGAAAGGGAAGCTGTCAGAGCAGTTAAAGCACTGCAACGGCATCCTGAAGGAACTGCTCTCAAAGAAGCACGCTGCCTACGCCTGGCCCTTCTATAAGCCAGTGGACGCTTCTGCTCTTGGCCTTCATGATTACCATGACATCATTAAACACCCCATGGACCTCAGCACTGTCAAGCGGAAGATGGAGAACCGTGACTACCGGGATGCACAGGAGTTTGCTGCTGATGTACGGCTTATGTTCTCCAACTGCTATAAGTACAATCCTCCAGACCACGATGTTGTGGCTATGGCACGAAAGTTGCAGGATGTGTTTGAGTTTCGCTATGCCAAGATGCCAGATGAGCCACTGGAACCAGGACCTCTACCAGTCTCTACTGCCTTGCCTCCTGGGTTGACCAAATCCTCTTCAGAGTCCTCCAGTGAGGAAAGTAGCAGTGAGAGTTCCTCTGAGGAAGAGGAGGAGGAGGAAGAAGATGAGGACGAGGAGGAGAGTGAAAGCTCAGACTCTGAGGAGGAAAGGGCTCATCGCCTAGCAGAGCTGCAGGAGCAGCTTCGGGCAGTTCATGAACAACTGGCTGCCCTGTCCCAGGGCCCAATATCTAAGCCCAAGCGGAAGAGAGAGAAAAAGGAAAAAAAGAAGAAACGGAAGGCAGAGAAACATCGTGGCCGAATTGGGATCGATGAAGATGATAAGGGGCCTAGGGCACCTCGCCCACCTCAGCCCAAGAAATCTAAGAAAGCAGGTGGTGGGGGTAGCAATGCTACTACACTCAGCCATCCTGGCTTTGGGACTTCCGGAGGAAGTAGCAACAAGCTACCTAAAAAGTCTCAAAAGACAGCTCCACCTGTCCTTCCCACTGGCTATGATTCTGAGGAGGAGGAAGAAAGCAGGCCCATGAGTTATGATGAGAAGAGACAGTTAAGCCTGGATATCAATAAGTTACCTGGGGAAAAGCTGGGTCGAGTAGTACATATCATCCAAGCCAGGGAACCCTCTCTACGTGATTCAAATCCAGAAGAAATTGAGATTGATTTTGAAACACTCAAGCCGTCCACACTTAGAGAGCTTGAGCGATATGTTTTATCCTGCCTTCGAAAGAAACCCCGGAAGCCCTACACTATTAGGAAACCTGTGGGAAAAACAAAGGAGGAACTGGCTTTGGAGAAGAAGCGGGAGCTAGAGAAGCGGTTGCAGGATGTCAGTGGACAGCTCAACTCCACCAAAAAGCCTCCCAAGAAAGCGAGTGAGAAGACAGAGTCATCTGCACAGCAAGTGGCAGTGTCCCGTCTCAGTGCTTCTAGTTCCAGCTCAGATTCCAGCTCCTCGTCGTCATCTTCCTCTTCTTCAGACACCAGCGATTCAGACTCGGGCTAAGCGGCCGC

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKGSMLQNVTPHKLPGEGNAGLLGLGPEAAAPGKRIRKPSLLYEGFESPTMASVPALQLAPANPPPPEVSNPKKPGRVTNQLQYLHKVVMKALWKHQFAWPFRQPVDAVKLGLPDYHKIIKQPMDMGTIKRRLENNYYWAASECMQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQELVVTIPKNSHKKGAKLAALQGSITSAHQVPAVSSVSHTALYTPPPEIPTTVLNIPHPSVISSPLLKSLHSAGPPLLAVSAAPPAQPLAKKKGVKRKADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKDLPDSQQQHQSSKKGKLSEQLKHCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHDIIKHPMDLSTVKRKMENRDYRDAQEFAADVRLMFSNCYKYNPPDHDVVAMARKLQDVFEFRYAKMPDEPLEPGPLPVSTALPPGLTKSSSESSSEESSSESSSEEEEEEEEDEDEEESESSDSEEERAHRLAELQEQLRAVHEQLAALSQGPISKPKRKREKKEKKKKRKAEKHRGRIGIDEDDKGPRAPRPPQPKKSKKAGGGGSNATTLSHPGFGTSGGSSNKLPKKSQKTAPPVLPTGYDSEEEEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLRDSNPEEIEIDFETLKPSTLRELERYVLSCLRKKPRKPYTIRKPVGKTKEELALEKKRELEKRLQDVSGQLNSTKKPPKKASEKTESSAQQVAVSRLSASSSSSDSSSSSSSSSSSDTSDSDSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55095
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Could you please clone mouse Brd2 into pCAGGs with an N-terminal FLAG tag? DU46360 might be used as template.