cDNA Clone WNK1

• Plasmid: pcDNA5D FRT/TO Halo WNK1 T1115-V1198 D1154A
• Parent Plasmid: pcDNA5D FRT/TO Halo
• Expressed: Halo WNK1 T1115-V1198 D1154AHALO-WNK1 (1115-1198) D1145A
• Genbank: NM_018979.3
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCACTTCACGCCCAAAATTAAGAATTTTGAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGACTCATAATAGGAAAATGGTTACATTCAAATTTGACCTAGATGGTGCCAACCCCGAGGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCGTTTGTGGATCAAGTGCGAGAAATTATTGAAAAAGCTGATGAAATGCTCAGTGAGGATGTCAGTGTGTAGGCGGCCGC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGPGSTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGANPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU26474
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Grow at or below 30ºC
• Low Temp: Yes