Plasmid
pET24-Ubiquitin D77 (silent a220c)
Parent Plasmid
pET24a original
Expressed
untagged Ubiquitin D77
Genbank
P62988*
Tag
untagged
Species
Human
Sequence of Insert
View Sequence
CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGGATTGATAAGGATCC
Amino Acid Sequence
View Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGGD**
EDGRTLSDYNIQKESTLHLVLRLRGGD**
Vector Type
Bacterial
Antibiotic
Kan
DU Number
DU28018
Unit Source
Location
PPU
MTA
Notes
Clone has C-terminal Asp added (D77)
Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA)
Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).
T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.
Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA)
Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).
T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.
Price
£125.00
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