cDNA Clone CTF4 (yeast)

• Plasmid: pGAD CTF4 (yeast) 1-927 A897E I901E
• Parent Plasmid: pGAD
• Expressed: AD CTF4 (yeast) 1-927 A897E I901E
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

CGGATCATCCTAAGCCCGAGAGATATATACTCCCATCACGACTCACTATAGGGCGAGCGCCGCCATGGAGTACCCATACGACGTACCAGATTACGCTCAT
ATGGCCATGGAGGCCAGGGAATTCCACCCGGGGGGAGCTGGGGCAGGCGCTGGAGCGGGGGCCATGGTTTCAGTTATAGACAAGCTTGTTTTTGACTTTG
GTGGGAAAACTCTGGTCTCTCTCGCACCAGATAATAATACTTTGTGTGTAGCCAATAAAAATGGCTTAACCAAGATTCTGAAGACAAATAACCCAGAAGA
AGAGCCAGAGACTTTAGATTCTTCCAAATTGGTCTCGTCTATAAAATGCTATTCAAACTCGCACTTTCTGATGACTACAATGCAAGGTGATGCTCTTAGG
TATAACATCGATTCTAGTCAAGAAGAATTATTGGCTAGATTTGCTTTACCCCTACGTGACTGCTGTGTTATTCATTCAGGTAAAATGGCCGTATTTGGAG
GAGACGACTTAGAACTAATCCTTTTGGAATTGGACGATGAAACACACAAAAAACATGCCATTAAAATCGATGAACAAGTTTCTCAAATTTCTTACAATTC
ACAGATGAATATTTTAGCAGTTTCAATGATAAATGGTAAGGTACAAATTTTTTCTCTGACATCTACTATTCCAAACAAAGTTCATGAGTTAAATGATTAC
ATAGTGGCCAATTCATATGATGATACACACAGAGATAAGATACTCTCGAATATGATGGATGATATAGATAAAGACAATGATAATGACCTGAGTGAAACGG
CTGATCCAGATGAGAACAATGTAGCTGATCCAGAATTCTGTGCTGCTAATAGAATTTGCACGAGAGTGGCTTGGCATCCGAAGGGTCTGCATTTTGCGCT
ACCATGTGCAGATGATACAGTAAAAATATTCTCTATAAAGGGATATTCCCTACAAAAGACGTTGTCCACAAATCTCTCATCAACAAAGGCTCATTTCATT
GATTTGCAATTTGACCCGTTACGTGGAACTTACATTGCGGCAGTAGATTTAAATAATAAGTTAACGGTATGGAATTGGGAAACTTCCGAGATCCACTACA
CCAGGGAATTCAAAAGAAAAATTACTAATATTGCCTGGAAAATCCAAGCGGACTCAAAAACTCTAGACCTTGTTTTAGGTACCTGGTCCGGTAGTATAGC
CATTGTCCAAAATTTGGCAGAGTCCGTAGTATCTAATATACCTGACCAATCTGTTGCTGAATCTTCAACTAAACATGGGCTTTTTGTAGACTCCGAATCT
GACTTGGAAAACTTAGAGGGAAATGATGATATAAACAAAAGCGATAAGCTATTTTCAGATATTACTCAAGAAGCGAATGCGGAAGATGTGTTCACTCAAA
CACACGACGGCCCCAGTGGATTAAGTGAAAAGAGAAAATACAACTTCGAAGATGAAGAAGACTTTATTGATGATGACGATGGTGCTGGTTATATTAGTGG
CAAAAAACCACATAATGAACATTCTTATTCGAGAGTACACAAGACTCATTCGTTTCCAATCAGTTTGGCAAACACAGGAAAATTTCGTTATATGCCTTTT
TCTCCAGCGGGAACACCTTTTGGCTTTACTGACAGGCGTTATTTGACAATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACAGCA
TAACTGTCTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCATTTCGAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATT
TGGCCAATCCAAAACTGGGCAGATACAATATAGGCCACACGATAGCATACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACA
AGTGTGGCAGCCACCCCGGTTCGCGTTATTGTTGGTACATCATTAGGCTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACAT
CCCCAATTGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTCAGTACATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTC
TAGTAAAAGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGTTTACCAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAATTTT
AATCCGATGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAGATCCATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGATCAC
CAGAAGAAAGTAAATGGCTCCCTATTCTAGATAGCAACATGGAAATATGGAAGATGTCAGGAGGGAAGGAAACGACAGATATACATGTCTGGCCCTTGGC
TTTGGCGTATGACACATTGAATTGTATCTTAGTTAAGGGCAAGCATATATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCA
GTATTTGTTAAGAGTAAATTACTAGAGGAAAACAAAGCTATATTAAATAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACA
AAGAAATACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTATCTGCGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGGTGA
AATGTACGGCAACGAAAATGAGGTATTGGCAGCATTGAACGGTGCATATGATAAGGCTTTGTTACGTTTATTTGCGTCTGCATGCTCAGACCAAAATGTT
GAAAAGGCTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAGCACTTACTGAAGCCGTTAAGGAATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAA
AAATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAAATAACTCGAGCTGCAGATGAATCGTAGATACTGAAAAACCCCGCAAGTCACTCAACT
GTGCATCGGCAGTAAT

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAMEYPYDVPDYAHMAMEAREFHPGGAGAGAGAGAMVSVIDKLVFDFGGKTLVS
LAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSIKCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCVIHSGKMAVFGGDDLELI
LLELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYIVANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDENN
VADPEFCAANRICTRVAWHPKGLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKLTVWNWETSEIHYTREFKRK
ITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIPDQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDITQEANAEDVFTQTHDGPSG
LSEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFD
VGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSPIVALT
AQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWL
PILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPV
SMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTEAVKESERAELPSLVKKINNIRE
ARYEQQLK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU51265
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee