cDNA Clone WNK1

• Synonyms: HSAN2, HSN2, KDP, PPP1R167, PRKWNK1, PSK, p65
• Plasmid: pcDNA5 frtTO FLAG WNK1 V403F
• Parent Plasmid: pcDNA5 frtTO
• Expressed: FLAG-WNK1 V403F
• Genbank: NM_018979.3
• Tag: FLAG
• Species: Human

Sequence of Insert

ATGTCTGGCGGCGCCGCAGAGAAGCAGAGCAGCACTCCCGG TTCCCTGTTCCTCTCGCCGCCGGCTCCTGCCCCCAAGAATGGCTCCAGCT CCGATTCCTCCGTGGGGGAGAAACTGGGAGCCGCGGCCGCCGACGCTGTG ACCGGCAGGACCGAGGAGTACAGGCGCCGCCGCCACACTATGGACAAGGA CAGCCGTGGGGCGGCCGCGACCACTACCACCACTGAGCACCGCTTCTTCC GCCGGAGCGTCATCTGCGACTCCAATGCCACTGCACTGGAGCTTCCCGGC CTTCCTCTTTCCCTGCCCCAGCCCAGCATCCCCGCGGCTGTCCCGCAGAG TGCTCCACCGGAGCCCCACCGGGAAGAGACCGTGACCGCCACCGCCACTT CCCAGGTAGCCCAGCAGCCTCCAGCCGCTGCCGCCCCTGGGGAACAGGCC GTCGCGGGCCCTGCCCCCTCGACTGTCCCCAGCAGTACCAGCAAAGACCG CCCAGTGTCCCAGCCTAGCCTTGTGGGGAGCAAAGAGGAGCCGCCGCCGG CGAGAAGTGGCAGCGGCGGCGGCAGCGCCAAGGAGCCACAGGAGGAACGG AGCCAGCAGCAGGATGATATCGAAGAGCTGGAGACCAAGGCCGTGGGAAT GTCTAACGATGGCCGCTTTCTCAAGTTTGACATCGAAATCGGCAGAGGCT CCTTTAAGACGGTCTACAAAGGTCTGGACACTGAAACCACCGTGGAAGTC GCCTGGTGTGAACTGCAGGATCGAAAATTAACAAAGTCTGAGAGGCAGAG ATTTAAAGAAGAAGCTGAAATGTTAAAAGGTCTTCAGCATCCCAATATTG TTAGATTTTATGATTCCTGGGAATCCACAGTAAAAGGAAAGAAGTGCATT GTTTTGGTGACTGAACTTATGACGTCTGGAACACTTAAAACGTATCTGAA AAGGTTTAAAGTGATGAAGATCAAAGTTCTAAGAAGCTGGTGCCGTCAGA TCCTTAAAGGTCTTCAGTTTCTTCATACTCGAACTCCACCTATCATTCAC CGCGATCTTAAATGTGACAACATCTTTATCACCGGCCCTACTGGCTCAGT CAAGATTGGAGACCTCGGTCTGGCAACCCTGAAGCGGGCTTCTTTTGCCA AGAGTGTGATAGGTACCCCAGAGTTCATGGCCCCTGAGATGTATGAGGAG AAATATGATGAATCCTTTGACGTTTATGCTTTTGGGATGTGCATGCTTGA GATGGCTACATCTGAATATCCTTACTCGGAGTGCCAAAATGCTGCGCAGA TCTACCGTCGCGTGACCAGTGGGGTGAAGCCAGCCAGTTTTGACAAAGTA GCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGCATACGACAAAACAA AGATGAAAGATATTCCATCAAAGACCTTTTGAACCATGCCTTCTTCCAAG AGGAAACAGGAGTACGGGTAGAATTAGCAGAAGAAGATGATGGAGAAAAA ATAGCCATAAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGGG AAAATACAAAGATAATGAAGCTATTGAGTTTTCTTTTGATTTAGAGAGAG ATGTCCCAGAAGATGTTGCACAAGAAATGGTAGAGTCTGGGTATGTCTGT GAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGACAGAGTATCATT AATTAAGAGGAAACGAGAGCAGCGGCAGTTGGTACGGGAGGAGCAAGAAA AAAAAAAGCAGGAAGAGAGCAGTCTCAAACAGCAGGTAGAACAATCCAGT GCTTCCCAGACAGGAATCAAGCAGCTCCCTTCTGCTAGCACCGGCATACC TACTGCTTCTACCACTTCAGCTTCAGTTTCTACACAAGTAGAACCTGAAG AACCTGAGGCAGATCAACATCAGCAACTACAGTACCAGCAACCCAGTATA TCTGTGTTATCTGATGGGACGGTTGACAGTGGTCAGGGATCCTCTGTCTT CACAGAATCTCGAGTGAGCAGCCAACAGACAGTTTCATATGGTTCCCAAC ATGAACAGGCACATTCTACAGGCACAGTCCCAGGGCATATACCTTCTACT GTCCAAGCACAGTCTCAGCCCCATGGGGTATATCCACCCTCAAGTGTGGC ACAGGGGCAGAGCCAGGGTCAGCCATCCTCAAGTAGCTTAACAGGGGTTT CATCTTCCCAACCCATACAACATCCTCAGCAGCAGCAGGGAATACAGCAG ACAGCCCCTCCTCAACAGACAGTGCAGTATTCACTTTCACAGACATCAAC CTCCAGTGAGGCCACTACTGCACAGCCAGTGAGTCAACCTCAAGCTCCAC AAGTCTTGCCTCAAGTATCAGCTGGAAAACAGCTTCCAGTTTCCCAGCCA GTACCAACTATCCAAGGCGAACCTCAGATCCCAGTTGCGACACAACCCTC GGTTGTTCCAGTCCACTCTGGTGCTCATTTCCTTCCAGTGGGACAGCCGC TCCCTACTCCCTTGCTCCCTCAGTACCCTGTCTCTCAGATTCCCATATCA ACTCCTCATGTGTCTACGGCTCAGACAGGTTTCTCATCCCTTCCCATCAC AATGGCAGCTGGCATTACTCAGCCTCTGCTCACGTTGGCTTCATCTGCTA CAACAGCTGCGATCCCGGGGGTATCAACTGTGGTTCCTAGTCAGCTTCCA ACCCTTCTGCAGCCTGTGACTCAGCTGCCAAGTCAGGTTCACCCACAGCT CCTACAACCAGCAGTTCAGTCCATGGGAATACCAGCTAACCTTGGACAAG CTGCTGAGGTTCCACTTTCCTCTGGAGATGTTCTGTACCAGGGCTTCCCA CCTCGACTGCCACCACAGTACCCAGGAGATTCAAATATTGCTCCCTCTTC CAACGTGGCTTCTGTTTGCATCCATTCTACAGTCCTATCCCCTCCCATGC CGACAGAAGTACTGGCTACACCTGGGTACTTTCCCACAGTGGTGCAGCCT TATGTGGAATCAAATCTTTTAGTTCCTATGGGTGGTGTAGGAGGACAGGT TCAAGTGTCCCAGCCAGGAGGGAGTTTAGCACAAGCCCCCACTACATCCT CCCAGCAAGCAGTTTTGGAGAGTACTCAGGGAGTCTCTCAGGTTGCTCCT GCAGAGCCAGTTGCAGTAGCACAGCCCCAAGCTACCCAGCCGACCACTTT GGCTTCCTCTGTAGACAGTGCACATTCAGATGTTGCTTCAGGTATGAGTG ATGGCAATGAGAACGTCCCATCTTCCAGTGGAAGGCATGAAGGAAGAACT ACAAAACGGCATTACCGAAAATCTGTAAGGAGTCGCTCTCGACATGAAAA AACTTCACGCCCAAAATTAAGAATTTTGAATGTTTCAAATAAAGGAGACC GAGTAGTAGAATGTCAATTAGAGACTCATAATAGGAAAATGGTTACATTC AAATTTGACCTAGATGGTGACAACCCCGAGGAGATAGCAACAATTATGGT GAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCGTTTGTGGATCAAG TGCGAGAAATTATTGAAAAAGCTGATGAAATGCTCAGTGAGGATGTCAGT GTGGAACCAGAGGGTGATCAGGGATTGGAGAGTCTACAAGGAAAGGATGA CTATGGCTTTTCAGGTTCTCAGAAGCTTGAAGGAGAGTTCAAACAACCAA TTCCTGCGTCTTCCATGCCACAGCAAATAGGCATTCCTACCAGTTCTTTA ACTCAAGTTGTTCATTCTGCGGGAAGGCGGTTTATAGTGAGTCCTGTGCC AGAAAGCCGATTACGAGAATCAAAAGTTTTCCCCAGTGAAATAACAGATA CAGTTGCTGCCTCTACAGCTCAGAGCCCTGGAATGAACTTGTCTCACTCT GCATCATCCCTTAGTCTACAACAGGCCTTTTCTGAACTTAGACGTGCCCA AATGACAGAAGGACCCAACACAGCACCTCCAAACTTTAGTCATACAGGAC CAACATTTCCAGTAGTACCTCCTTTCTTAAGTAGCATTGCTGGAGTCCCA ACCACAGCAGCAGCCACAGCACCAGTCCCTGCAACAAGCAGCCCTCCTAA TGACATTTCCACATCAGTAATTCAGTCTGAGGTTACAGTGCCCACTGAAG AGGGGATTGCTGGAGTTGCCACCAGCACAGGTGTGGTAACTTCGGGTGGT CTCCCCATACCACCTGTGTCTGAATCACCAGTACTTTCCAGCGTAGTTTC AAGTATCACAATACCTGCAGTTGTCTCAATATCTACTACATCCCCGTCAC TTCAAGTCCCCACATCCACATCTGAGATCGTTGTTTCTAGTACAGCACTG TATCCTTCAGTAACAGTTTCAGCAACTTCAGCCTCTGCAGGGGGCAGTAC TGCTACCCCAGGTCCTAAGCCTCCAGCTGTAGTATCTCAGCAGGCAGCAG GCAGCACTACTGTGGGAGCCACATTAACATCAGTTTCTACCACCACTTCA TTCCCAAGCACAGCTTCACAGCTGTCCATTCAGCTTAGCAGCAGTACTTC TACTCCTACTTTAGCTGAAACCGTGGTAGTTAGCGCACACTCACTAGATA AGACATCTCATAGCAGTACAACTGGATTGGCTTTCTCCCTCTCTGCACCA TCTTCCTCTTCCTCTCCTGGAGCAGGAGTGTCTAGTTATATTTCTCAGCC TGGTGGGCTGCATCCTTTGGTCATTCCATCAGTGATAGCTTCTACTCCTA TTCTTCCCCAAGCAGCAGGACCTACTTCTACACCTTTATTACCCCAAGTA CCTAGTATCCCACCCTTGGTACAGCCTGTTGCCAATGTGCCTGCTGTACA GCAGACACTAATTCATAGTCAGCCTCAACCAGCTTTGCTTCCCAACCAGC CCCATACTCATTGTCCTGAAGTAGATTCTGATACACAACCCAAAGCTCCT GGAATTGATGACATAAAGACTCTAGAAGAAAAGCTGCGGTCTCTGTTCAG TGAACACAGCTCATCTGGAGCTCAGCATGCCTCTGTCTCACTGGAGACCT CACTAGTCATAGAGAGCACTGTCACACCAGGCATCCCAACTACTGCTGTT GCACCAAGCAAACTCCTGACTTCTACCACAAGTACTTGCTTACCACCAAC CAATTTACCACTAGGAACAGTTGCTTTGCCAGTTACACCAGTGGTCACAC CTGGGCAAGTTTCTACCCCAGTCAGCACTACTACATCAGGAGTGAAACCT GGAACTGCTCCCTCCAAGCCACCTCTAACTAAGGCTCCGGTGCTGCCAGT GGGTACTGAACTTCCAGCAGGTACTCTACCCAGCGAGCAGCTGCCACCTT TTCCAGGACCTTCTCTAACCCAGTCCCAGCAACCTCTAGAGGATCTTGAT GCTCAATTGAGAAGAACACTTAGTCCAGAGATTATCACAGTGACTTCTGC GGTTGGTCCTGTGTCCATGGCGGCTCCAACAGCAATCACAGAAGCAGGAA CACAGCCTCAGAAGGGTGTTTCTCAAGTCAAAGAAGGCCCTGTCCTAGCG ACTAGTTCAGGAGCTGGTGTTTTTAAGATGGGACGATTTCAGGTTTCTGT TGCAGCAGACGGTGCCCAGAAAGAGGGTAAAAATAAGTCAGAAGATGCAA AGTCTGTTCATTTTGAATCCAGCACCTCAGAGTCCTCAGTGCTATCAAGT AGTAGTCCAGAGAGTACCTTGGTGAAACCAGAGCCGAATGGCATAACCAT CCCTGGTATCTCTTCAGATGTGCCAGAGAGTGCCCACAAAACTACTGCCT CAGAGGCAAAGTCAGACACTGGGCAGCCTACCAAGGTTGGACGTTTTCAG GTGACAACTACAGCAAACAAAGTGGGTCGTTTCTCTGTATCAAAAACTGA GGACAAGATCACTGACACAAAGAAAGAAGGACCAGTGGCATCTCCTCCTT TTATGGATTTGGAACAAGCTGTTCTTCCTGCTGTGATACCAAAGAAAGAG AAGCCTGAACTGTCAGAGCCTTCACATCTAAATGGGCCGTCTTCTGACCC GGAGGCCGCTTTTTTAAGTAGGGATGTGGATGATGGTTCCGGTAGTCCAC ACTCGCCCCATCAGCTGAGCTCAAAGAGCCTTCCTAGCCAGAATCTAAGT CAAAGCCTTAGTAATTCATTTAACTCCTCTTACATGAGTAGCGACAATGA GTCAGATATCGAAGATGAAGACTTAAAGTTAGAGCTGCGACGACTACGAG ATAAACATCTCAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAA ATTGAATCTTTGTATACCAAACTGGGCAAGGTGCCCCCTGCTGTTATTAT TCCCCCAGCTGCTCCCCTTTCAGGGAGAAGACGACGACCCACTAAAAGCA AAGGCAGCAAATCTAGTCGAAGCAGTTCCTTGGGGAATAAAAGCCCCCAG CTTTCAGGTAACCTGTCTGGTCAGAGTGCAGCTTCAGTCTTGCACCCCCA GCAGACCCTCCACCCTCCTGGCAACATCCCAGAGTCCGGGCAGAATCAGC TGTTACAGCCCCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCC TTCACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCA AGGAACCAGCAGCACAAACACTGTTGGGGCAACAGTGAACAGCCAAGCCG CCCAAGCTCAGCCTCCTGCCATGACGTCCAGCAGGAAGGGCACATTCACA GATGACTTGCACAAGTTGGTAGACAATTGGGCCCGAGATGCCATGAATCT CTCAGGCAGGAGAGGAAGCAAAGGGCACATGAATTATGAGGGCCCTGGAA TGGCAAGGAAGTTCTCTGCACCTGGGCAACTGTGCATCTCCATGACCTCG AACCTGGGTGGCTCTGCCCCCATCTCTGCAGCATCAGCTACCTCTCTAGG TCACTTCACCAAGTCTATGTGCCCCCCACAGCAGTATGGCTTTCCAGCTA CCCCATTTGGCGCTCAATGGAGTGGGACGGGTGGCCCAGCACCACAGCCA CTTGGCCAGTTCCAACCTGTGGGAACTGCCTCCTTGCAGAATTTCAACAT CAGCAATTTGCAGAAATCCATCAGCAACCCCCCAGGCTCCAACCTGCGGA CCACTTAG

Amino Acid Sequence

MDYKDDDDKSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATA
LELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKE
PQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVK
GKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAP
EMYEEKYDESFDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEE
DDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQ
VEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPG
HIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQL
PVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVV
PSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFP
TVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGR
HEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEML
SEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGM
NLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGV
VTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSV
STTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTP
LLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGI
PTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQP
LEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSES
SVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAV
IPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQS
RQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSD
NLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLC
ISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU52691
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: V403F.; Contains several silent variations as well as two benign residue changes (according to ensemble); T1056P and M1808I. All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.