cDNA Clone Ctf4

• Plasmid: pGAD CTF4 (yeast) 1-927L867E I901E
• Parent Plasmid: pGAD
• Expressed: GalAD-Ctf4(1-927) L867E I901E
• Genbank: NP_015461.1
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

GAGCCAGAGACTTTAGATTCTTCCAAATTGGTCTCGTCTATAAAATGCTATTCAAACTCGCACTTTCTGATGACTACAATGCAAGGTGATGCTCTTAGGT
ATAACATCGATTCTAGTCAAGAAGAATTATTGGCTAGATTTGCTTTACCCCTACGTGACTGCTGTGTTATTCATTCAGGTAAAATGGCCGTATTTGGAGG
AGACGACTTAGAACTAATCCTTTTGGAATTGGACGATGAAACACACAAAAAACATGCCATTAAAATCGATGAACAAGTTTCTCAAATTTCTTACAATTCA
CAGATGAATATTTTAGCAGTTTCAATGATAAATGGTAAGGTACAAATTTTTTCTCTGACATCTACTATTCCAAACAAAGTTCATGAGTTAAATGATTACA
TAGTGGCCAATTCTTATGATGATACACACAGAGATAAGATACTCTCGAATATGATGGATGATATAGATAAAGACAATGATAATGACCTGAGTGAAACGGC
TGATCCAGATGAGAACAATGTAGCTGATCCAGAATTCTGTGCTGCTAATAGAATTTGCACGAGAGTGGCTTGGCATCCGAAGGGTCTGCATTTTGCGCTA
CCATGTGCAGATGATACAGTAAAAATATTCTCTATAAAGGGATATTCCCTACAAAAGACGTTGTCCACAAATCTCTCATCAACAAAGGCTCATTTCATTG
ATTTGCAATTTGACCCGTTACGTGGAACTTACATTGCGGCAGTAGATTTAAATAATAAGTTAACGGTATGGAATTGGGAAACTTCCGAGATCCACTACAC
CAGGGAATTCAAAAGAAAAATTACTAATATTGCCTGGAAAATCCAAGCGGACTCAAAAACTCTAGACCTTGTTTTAGGTACCTGGTCCGGTAGTATAGCC
ATTGTCCAAAATTTGGCAGAGTCCGTAGTATCTAATATACCTGACCAATCTGTTGCTGAATCTTCAACTAAACATGGGCTTTTTGTAGACTCCGAATCTG
ACTTGGAAAACTTAGAGGGAAATGATGATATAAACAAAAGCGATAAGCTATTTTCAGATATTACTCAAGAAGCGAATGCGGAAGATGTGTTCACTCAAAC
ACACGACGGCCCCAGTGGATTAAGTGAAAAGAGAAAATACAACTTCGAAGATGAAGAAGACTTTATTGATGATGACGATGGTGCTGGTTATATTAGTGGC
AAAAAACCACATAATGAACATTCTTATTCGAGAGTACACAAGACTCATTCGTTTCCAATCAGTTTGGCAAACACAGGAAAATTTCGTTATATGCCTTTTT
CTCCAGCGGGAACACCTTTTGGCTTTACTGACAGGCGTTATTTGACAATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACAGCAT
AACTGTCTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCATTTCGAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATTT
GGCCAATCCAAAACTGGGCAGATACAATATAGGCCACACGATAGCATACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACAA
GTGTGGCAGCCACCCCGGTTCGCGTTATTGTTGGTACATCATTAGGCTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACATC
CCCAATTGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTCAGTACATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTCT
AGTAAAAGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGTTTACCAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAATTTTA
ATCCGATGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAGATCCATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGATCACC
AGAAGAAAGTAAATGGCTCCCTATTCTAGATAGCAACATGGAAATATGGAAGATGTCAGGAGGGAAGGAAACGACAGATATACATGTCTGGCCCTTGGCT
TTGGCGTATGACACATTGAATTGTATCTTAGTTAAGGGCAAGCATATATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCAG
TATTTGTTAAGAGTAAATTACTAGAGGAAAACAAAGCTATATTAAATAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACAA
AGAAATACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTATCTGCGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGGTGAA
ATGTACGGCAACGAAAATGAGGTATTGGCAGCATTGAACGGTGCATATGATAAGGCTTTGGAACGTTTATTTGCGTCTGCATGCTCAGACCAAAATGTTG
AAAAGGCTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAGCACTTACTGCAGCCGTTAAGGAATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAAA
AATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAAATAACTCGAG

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAMEYPYDVPNYAHMAMEASEFHPGGAGAGAGAGAMVSVIDKLVFDFGGKTLVS
LAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSIKCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCVIHSGKMAVFGGDDLELI
LLELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYIVANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDENN
VADPEFCAANRICTRVAWHPKGLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKLTVWNWETSEIHYTREFKRK
ITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIPDQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDITQEANAEDVFTQTHDGPSG
LSEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFD
VGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSPIVALT
AQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWL
PILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPV
SMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALERLFASACSDQNVEKALSLAHELKQDRALTAAVKESERAELPSLVKKINNIRE
ARYEQQLK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU51214
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee