cDNA Clone USP11

• Plasmid: pGEX6P1 USP11 R418Q
• Parent Plasmid: pGEX6P1
• Expressed: USP11-R418Q

Sequence of Insert

GCGGCCGCATGGCAGTAGCCCCGCGACTGTTTGGGGGGCTCTGCTTCCGTTTCCGGGACCAGAATCCGGAAGTGGCTGTTGAGGGGCGTCTTCCAATCTCGCACAGCTGCGTTGGCTGTAGAAGAGAACGGACGGCGATGGCGACGGTCGCAGCAAATCCAGCTGCTGCTGCGGCGGCTGTGGCGGCGGCAGCGGCGGTGACTGAGGATAGAGAGCCACAGCACGAGGAGCTGCCAGGCCTGGACAGCCAGTGGCGCCAGATAGAAAACGGCGAGAGTGGGCGAGAACGTCCACTGCGGGCCGGCGAAAGCTGGTTCCTTGTGGAGAAGCACTGGTATAAGCAGTGGGAGGCATACGTGCAGGGAGGGGACCAGGACTCCAGCACCTTCCCTGGCTGCATCAACAATGCCACACTCTTTCAAGATGAGATAAACTGGCGCCTCAAGGAGGGACTGGTGGAAGGCGAGGATTATGTGCTGCTCCCAGCAGCTGCTTGGCATTACCTGGTCAGCTGGTATGGTCTAGAGCATGGCCAGCCACCCATTGAACGCAAGGTCATAGAGCTGCCCAACATCCAGAAGGTCGAAGTGTACCCAGTAGAACTGCTGCTTGTCCGGCACAATGATTTGGGCAAATCTCACACTGTTCAGTTCAGCCATACCGATTCTATTGGCCTAGTATTGCGCACAGCTCGGGAGCGGTTTCTGGTGGAGCCCCAGGAAGACACTCGGCTTTGGGCCAAGAACTCAGAAGGCTCTTTGGATAGGTTGTATGACACACACATCACGGTTCTCGATGCGGCCCTTGAGACTGGGCAGTTGATCATCATGGAGACCCGCAAGAAAGATGGCACTTGGCCCAGCGCACAGCTGCATGTCATGAACAACAACATGTCGGAAGAGGATGAGGACTTCAAGGGTCAGCCAGGCATCTGTGGCCTCACCAATCTGGGCAACACGTGCTTCATGAACTCGGCCCTGCAGTGCCTCAGCAATGTGCCACAGCTCACCGAGTACTTCCTCAACAACTGCTACCTGGAGGAGCTCAACTTCCGCAACCCACTGGGCATGAAGGGTGAGATCGCAGAGGCCTATGCAGACCTGGTGAAGCAGGCGTGGTCTGGCCACCACCGCTCCATTGTGCCACATGTGTTCAAGAACAAGGTTGGCCATTTTGCATCCCAATTTCTGGGCTACCAGCAGCATGACTCTCAGGAGCTGCTGTCATTCCTCCTGGACGGGCTGCATGAGGACCTTAATCaGGTGAAGAAGAAGGAGTATGTGGAGCTGTGCGATGCTGCTGGGCGACCGGATCAGGAGGTGGCACAGGAGGCATGGCAAAACCACAAACGGCGGAACGATTCTGTGATCGTGGACACTTTCCACGGCCTCTTCAAGTCCACGCTGGTGTGCCCCGATTGTGGCAATGTATCTGTGACCTTCGACCCCTTCTGCTACCTCAGTGTTCCACTGCCTATCAGCCACAAGAGGGTCTTGGAGGTCTTCTTTATCCCCATGGATCCGCGCCGCAAGCCAGAGCAGCACCGGCTCGTGGTCCCCAAGAAAGGCAAGATCTCGGATCTATGTGTGGCTCTGTCCAAACACACGGGCATCTCGCCAGAGAGGATGATGGTGGCTGATGTCTTCAGTCACCGCTTCTATAAGCTCTATCAGCTAGAGGAGCCTCTGAGCAGCATCTTGGACCGTGATGATATCTTCGTCTATGAGGTGTCAGGTCGCATTGAGGCCATTGAGGGCTCAAGAGAGGACATCGTGGTTCCTGTCTACCTGCGGGAGCGCACCCCTGCCCGTGACTACAACAACTCCTACTACGGCCTGATGCTTTTTGGACACCCCCTCCTGGTATCAGTGCCCCGGGACCGCTTCACCTGGGAGGGCCTGTATAACGTCCTGATGTACCGGCTCTCACGCTACGTGACCAAACCCAACTCAGATGATGAGGACGATGGGGATGAGAAAGAAGATGACGAGGAGGATAAAGATGACGTCCCTGGGCCCTCAACTGGGGGCAGCCTCCGAGACCCTGAGCCAGAGCAGGCTGGGCCCAGCTCTGGAGTCACGAACAGGTGCCCGTTCCTCCTGGACAATTGCCTTGGCACATCTCAGTGGCCCCCAAGGCGACGACGCAAGCAGCTGTTCACCCTGCAGACGGTGAACTCCAATGGGACCAGCGACCGCACAACCTCCCCTGAAGAAGTCCATGCCCAGCCGTACATTGCTATCGACTGGGAGCCAGAGATGAAGAAGCGTTACTATGACGAGGTAGAGGCTGAGGGCTACGTGAAGCATGACTGCGTCGGGTACGTGATGAAGAAGGCTCCCGTGCGGCTGCAGGAGTGCATTGAGCTCTTCACCACTGTGGAGACCCTGGAGAAGGAAAACCCCTGGTACTGCCCTTCCTGCAAGCAGCACCAGCTGGCAACCAAGAAGCTGGACCTGTGGATGCTGCCGGAGATTCTCATCATCCACCTGAAACGCTTTTCCTACACCAAGTTCTCCCGAGAGAAGCTGGACACCCTCGTGGAGTTTCCTATCCGGGACCTGGACTTCTCTGAGTTTGTCATCCAGCCACAGAATGAGTCGAATCCGGAGCTGTACAAATATGACCTCATCGCGGTTTCCAACCATTATGGGGGCATGCGTGATGGACACTACACAACATTTGCCTGCAACAAGGACAGCGGCCAGTGGCACTACTTTGATGACAACAGCGTCTCCCCTGTCAATGAGAATCAGATCGAGTCCAAGGCAGCCTATGTCCTCTTCTACCAACGCCAGGACGTGGCGCGACGCCTGCTGTCCCCGGCCGGCTCATCTGGCGCCCCAGCCTCCCCTGCCTGCAGCTCCCCACCCAGCTCTGAGTTCATGGATGTTAATTGAGCGGCCGC

• Vector Type: Bacterial
• DU Number: DU47586
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please subclone USP11 R418Q (DU42351) onto a bacterial expression vector and pass it to James/Axel for protein production. This is a pathogenic mutant and we want to test its activity.