cDNA Clone BMH2 (yeast)

• Plasmid: pET 6HIS HALO BMH2 (yeast)
• Parent Plasmid: pET15b 6xHIS TEV HALO Prec
• Expressed: His HALO BMH2
• Tag: HALO
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCATGTCCCAAACTCGTGAAGATTCTGTTTACCTAGCTAAATTAGC TGAACAAGCCGAACGTTATGAAGAAATGGTCGAAAACATGAAGGCCGTTG CTTCATCAGGTCAAGAGTTATCTGTCGAAGAACGGAATCTATTGTCGGTT GCTTACAAGAACGTCATCGGTGCTCGCCGTGCTTCATGGAGAATAGTTTC TTCGATCGAACAAAAAGAAGAATCAAAGGAGAAATCTGAACATCAAGTTG AATTAATCCGTTCTTACCGTTCTAAAATTGAAACTGAATTGACCAAAATC TCTGACGACATTTTATCTGTGTTAGATTCTCATTTAATCCCTTCTGCTAC TACTGGTGAGTCTAAAGTATTTTACTATAAGATGAAGGGTGACTACCACC GTTATTTAGCTGAATTTTCCAGCGGAGATGCAAGAGAAAAGGCAACCAAC TCCTCTTTGGAGGCTTATAAAACCGCTTCCGAAATCGCCACAACTGAATT GCCTCCAACTCACCCAATTCGTTTAGGTCTAGCTTTGAATTTCTCCGTCT TCTATTACGAAATTCAAAACTCTCCTGATAAGGCTTGCCACTTGGCCAAA CAAGCCTTTGATGATGCTATTGCTGAGTTAGATACTTTATCTGAAGAATC ATACAAGGATAGCACTTTGATCATGCAATTATTAAGGGACAACTTGACCT TATGGACCTCTGATATTTCTGAATCTGGTCAAGAAGATCAACAACAACAA CAACAACAGCAACAGCAACAGCAACAACAGCAACAACAAGCTCCAGCTGA ACAAACTCAAGGTGAACCAACCAAATAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSMSQTREDSVYLAKLAEQAERYEEMVENMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRI
VSSIEQKEESKEKSEHQVELIRSYRSKIETELTKISDDILSVLDSHLIPSATTGESKVFYYKMKGDYHRYLAEFSSGDAREKATNSSLEAYKTASEIATT
ELPPTHPIRLGLALNFSVFYYEIQNSPDKACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDISESGQEDQQQQQQQQQQQQQQQQQAP
AEQTQGEPTK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51278
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: For purification of HALO-BMH2 from bacteria