Plasmid
pGAD CTF4 (yeast) 461-927 A897E
Parent Plasmid
pGAD
Expressed
GalAD-Ctf4(461-927)-A897E
Genbank
NP_015461.1
Tag
GAL4 DNA-AD
Sequence of Insert
View Sequence
ACTCATTCGTTTCCAATCAGTTTGGCAAACACAGGAAAATTTCGTTATATGCCTTTTTCTCCAGCGGGAACACCTTTTGGCTTTACTGACAGGCGTTATT
TGACAATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACAGCATAACTGTCTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCA
TTTCGAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATTTGGCCAATCCAAAACTGGGCAGATACAATATAGGCCACACGAT
AGCATACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACAAGTGTGGCAGCCACCCCGGTTCGCGTTATTGTTGGTACATCAT
TAGGCTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACATCCCCAATTGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTC
AGTACATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTCTAGTAAAAGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGT
TTACCAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAATTTTAATCCGATGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAG
ATCCATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGATCACCAGAAGAAAGTAAATGGCTCCCTATTCTAGATAGCAACATGGA
AATATGGAAGATGTCAGGAGGGAAGGAAACGACAGATATACATGTCTGGCCCTTGGCTTTGGCGTATGACACATTGAATTGTATCTTAGTTAAGGGCAAG
CATATATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCAGTATTTGTTAAGAGTAAATTACTAGAGGAAAACAAAGCTATAT
TAAATAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACAAAGAAATACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTA
TCTGCGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGGTGAAATGTACGGCAACGAAAATGAGGTATTGGCAGCATTGAACGGT
GCATATGATAAGGCTTTGTTACGTTTATTTGCGTCTGCATGCTCAGACCAAAATGTTGAAAAGGCTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAG
CACTTACTGAAGCCGTTAAGATATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAAAAATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAA
ATGA
TGACAATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACAGCATAACTGTCTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCA
TTTCGAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATTTGGCCAATCCAAAACTGGGCAGATACAATATAGGCCACACGAT
AGCATACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACAAGTGTGGCAGCCACCCCGGTTCGCGTTATTGTTGGTACATCAT
TAGGCTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACATCCCCAATTGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTC
AGTACATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTCTAGTAAAAGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGT
TTACCAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAATTTTAATCCGATGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAG
ATCCATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGATCACCAGAAGAAAGTAAATGGCTCCCTATTCTAGATAGCAACATGGA
AATATGGAAGATGTCAGGAGGGAAGGAAACGACAGATATACATGTCTGGCCCTTGGCTTTGGCGTATGACACATTGAATTGTATCTTAGTTAAGGGCAAG
CATATATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCAGTATTTGTTAAGAGTAAATTACTAGAGGAAAACAAAGCTATAT
TAAATAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACAAAGAAATACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTA
TCTGCGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGGTGAAATGTACGGCAACGAAAATGAGGTATTGGCAGCATTGAACGGT
GCATATGATAAGGCTTTGTTACGTTTATTTGCGTCTGCATGCTCAGACCAAAATGTTGAAAAGGCTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAG
CACTTACTGAAGCCGTTAAGATATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAAAAATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAA
ATGA
Amino Acid Sequence
View Sequence
MVSVIDKLVFDFGGKTLVSLAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSIKCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDC
CVIHSGKMAVFGGDDLELILLELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYIVANSYDDTHRDKILSNMMDD
IDKDNDNDLSETADPDENNVADPEFCAANRICTRVAWHPKGLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKL
TVWNWETSEIHYTREFKRKITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIPDQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDI
TQEANAEDVFTQTHDGPSGLSEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVG
YVSTVKNSEQYSITVSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSF
NQFGVPFAVEKTSPIVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGS
DNTLLLLSKWRSPEESKWLPILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEI
GADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVK
ISERAELPSLVKKINNIREARYEQQLK
CVIHSGKMAVFGGDDLELILLELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYIVANSYDDTHRDKILSNMMDD
IDKDNDNDLSETADPDENNVADPEFCAANRICTRVAWHPKGLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKL
TVWNWETSEIHYTREFKRKITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIPDQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDI
TQEANAEDVFTQTHDGPSGLSEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVG
YVSTVKNSEQYSITVSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSF
NQFGVPFAVEKTSPIVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGS
DNTLLLLSKWRSPEESKWLPILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEI
GADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVK
ISERAELPSLVKKINNIREARYEQQLK
Vector Type
Yeast
Antibiotic
Amp
DU Number
DU51217
Unit Source
Location
PPU
MTA
Notes
I would like a plasmid carrying the fragment Ctf4 (461-927) mutated in A897E. I will provide the initial plasmid (amp resistance) and a DNA strider file with the exact sequence for the final clone.
many thanks
Fabrizio
many thanks
Fabrizio
Price
£125.00
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