Plasmid
pGAD CTF4 (yeast) 461-927 A871E
Parent Plasmid
pGAD
Expressed
GalAD-Ctf4(461-927)-A871E
Genbank
NP_015461.1
Tag
GAL4 DNA-BD
Sequence of Insert
View Sequence
ACTCATTCGTTTCCAATCAGTTTGGCAAACACAGGAAAATTTCGTTATATGCCTTTTTCTCCAGCGGGAACACCTTTTGGCTTTACTGACAGGCGTTATT
TGACAATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACAGCATAACTGTCTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCA
TTTCGAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATTTGGCCAATCCAAAACTGGGCAGATACAATATAGGCCACACGAT
AGCATACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACAAGTGTGGCAGCCACCCCGGTTCGCGTTATTGTTGGTACATCAT
TAGGCTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACATCCCCAATTGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTC
AGTACATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTCTAGTAAAAGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGT
TTACCAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAATTTTAATCCGATGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAG
ATCCATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGATCACCAGAAGAAAGTAAATGGCTCCCTATTCTAGATAGCAACATGGA
AATATGGAAGATGTCAGGAGGGAAGGAAACGACAGATATACATGTCTGGCCCTTGGCTTTGGCGTATGACACATTGAATTGTATCTTAGTTAAGGGCAAG
CATATATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCAGTATTTGTTAAGAGTAAATTACTAGAGGAAAACAAAGCTATAT
TAAATAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACAAAGAAATACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTA
TCTGCGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGGTGAAATGTACGGCAACGAAAATGAGGTATTGGCAGCATTGAACGGT
GCATATGATAAGGCTTTGTTACGTTTATTTGAGTCTGCATGCTCAGACCAAAATGTTGAAAAGGCTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAG
CACTTACTGCAGCCGTTAAGATATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAAAAATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAA
ATGA
TGACAATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACAGCATAACTGTCTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCA
TTTCGAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATTTGGCCAATCCAAAACTGGGCAGATACAATATAGGCCACACGAT
AGCATACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACAAGTGTGGCAGCCACCCCGGTTCGCGTTATTGTTGGTACATCAT
TAGGCTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACATCCCCAATTGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTC
AGTACATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTCTAGTAAAAGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGT
TTACCAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAATTTTAATCCGATGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAG
ATCCATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGATCACCAGAAGAAAGTAAATGGCTCCCTATTCTAGATAGCAACATGGA
AATATGGAAGATGTCAGGAGGGAAGGAAACGACAGATATACATGTCTGGCCCTTGGCTTTGGCGTATGACACATTGAATTGTATCTTAGTTAAGGGCAAG
CATATATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCAGTATTTGTTAAGAGTAAATTACTAGAGGAAAACAAAGCTATAT
TAAATAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACAAAGAAATACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTA
TCTGCGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGGTGAAATGTACGGCAACGAAAATGAGGTATTGGCAGCATTGAACGGT
GCATATGATAAGGCTTTGTTACGTTTATTTGAGTCTGCATGCTCAGACCAAAATGTTGAAAAGGCTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAG
CACTTACTGCAGCCGTTAAGATATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAAAAATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAA
ATGA
Amino Acid Sequence
View Sequence
MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEILELAMAGRATHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSIT
VSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSP
IVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPE
ESKWLPILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEIGADTEAEEGEEDKE
IQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFESACSDQNVEKALSLAHELKQDRALTAAVKISERAELPSLVKKI
NNIREARYEQQLK*
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEILELAMAGRATHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSIT
VSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSP
IVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPE
ESKWLPILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEIGADTEAEEGEEDKE
IQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFESACSDQNVEKALSLAHELKQDRALTAAVKISERAELPSLVKKI
NNIREARYEQQLK*
Vector Type
Yeast
Antibiotic
Amp
DU Number
DU51216
Unit Source
Location
PPU
MTA
Notes
I would like a plasmid carrying the fragment Ctf4 (461-927) mutated in A871E. I will provide the initial plasmid (amp resistance) and a DNA strider file with the exact sequence for the final clone.
many thanks
Fabrizio
many thanks
Fabrizio
Price
£125.00
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