cDNA Clone X.l. LRR-1

• Plasmid: pET 6HIS SUMO LRR1 (xenopus)
• Parent Plasmid: pET156P
• Expressed: 6His-SUMO-Xenopus_LRR-1
• Species: Xenopus laevis

Sequence of Insert

CCATGGGTCATCATCATCACCATCATAGCGATCAAGAAGCAAAACCGAGCACCGAAGATCTGGGTGATAAAAAAGAAGGCGAGTATATCAAACTGAAAGT
GATTGGTCAGGATAGCAGCGAAATCCATTTTAAAGTTCAGATGACCACCCATCTGAAAAAACTGAAAGAAAGCTATTGTCAGCGTCAGGGTGTTCCGATG
AATAGCCTGCGTTTTCTGTTTGAAGGTCAGCGTATTGCAGATAATCATACCCCGAAAGAACTGGGTATGGAAGAGGAAGATGTGATCGAAGTTTATCAAG
AACAGACCGGTGGTATGAAACTGCAGTGTGAAGTTGAAGTGATTAATCGTATGCTGCCGACCTTTGGTCTGAAAAATCGTGGTAAAGGCACCCGTGCAGT
TCTGAGCGTTGGTCGTCAAGAGGGTAAACGTGGTGCAGCATATCTGATGATTTGTACCCTGAAAGATAAAAGCGGTAGCCGCTATAAACTGGAAAACAAT
ATTGAGCAGCTGTTTACCCGTTTTGTGGGTGAAGGTAAAGCAACCCTGCGTCTGAAAGAACCGGCACTGGATATTTGTCTGAGCAAAGCAGAAATTTGTG
GTCTGCGTAACTTTATTAGCACCGTTGGTCTGGCAAATAAAGGTACAGATATTGGCACCGTTAGCCTGCCTCGTCTGACACCGGCAAAAACCAGCGAAAT
TGAAAAACCGCGTAGCAAACTGTTTATCACCACCAAAAAAGATTATCCGATCACCAAAAGCTTTCCGTATAGCCTGGAACATCTGCAGGTTAGCTATTGT
AAACTGGCACGTGTTGATATGCGTATGCTGTGCCTGAAAAAACTGCAGAAACTGGATCTGAGCAACAACCACATCAAAAAACTGCCTAAAACCATTGGTG
ATCTGGTTTGTCTGCAAGAACTGATTCTGAACCATAACTTTCTGGAATCCTTTGAAGTTGTTCTGTGTAGCACCACCCTGCGTGATACACTGAAAAGCCT
GGATCTGTCAGCAAATAAACTGAAAGCACTGCCGGTTCAGATCTGCAATTTCAAAGAGCTGGTTAGCCTGAAACTGGACGAAAATGAACTGCTGCAGCTG
CCGTTTCCGATTGGCCAGCTGAGCAAACTGCGCTTTCTGAGCGCAACCAAAAACAAACTGCAATGTCTGCCGAACACCTTCAAAAAACTGACCCTGGAAA
ATCTGGACCTGTTTGGTAATCCGTTTATGCAGGCAACACCGCTGGTTCCGGATATCCAGCTGAAAATTCCGCTGCCGCTGCTGGAAACCGCAGCACGTGC
AACCCTGAAATATCGTATTCCGTATGGTCCGCATCTGATTCCGGCAACCCTGTGTCAGGATCTGAGTCTGGCCAAAACCTGTGATTGCGGTCTGCCGTGT
CTGAATAGCTTTATTCAGACCGTGGTTCTGATGAATCTGCATCAGGTTAGCCAGACCGTTGTGCTGGTTGATACAATGGGTGGCACCGATGGTCCGATTG
TTTGTTATTTTTGTAGCCTGACCTGCTATAGCCAGTTCCTGGATAAATATCTGCAGAGCACCCGTGTTTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVQMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMKLQCEVEVINRMLPTFGLKNRGKGTRAVLSVGRQEGKRGAAYLMICTLKDKSGSRYKLENNIEQLFTRFVGEGKATLRLKEPALDICLSKAEICG
LRNFISTVGLANKGTDIGTVSLPRLTPAKTSEIEKPRSKLFITTKKDYPITKSFPYSLEHLQVSYCKLARVDMRMLCLKKLQKLDLSNNHIKKLPKTIGD
LVCLQELILNHNFLESFEVVLCSTTLRDTLKSLDLSANKLKALPVQICNFKELVSLKLDENELLQLPFPIGQLSKLRFLSATKNKLQCLPNTFKKLTLEN
LDLFGNPFMQATPLVPDIQLKIPLPLLETAARATLKYRIPYGPHLIPATLCQDLSLAKTCDCGLPCLNSFIQTVVLMNLHQVSQTVVLVDTMGGTDGPIV
CYFCSLTCYSQFLDKYLQSTRV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51191
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Codon optimized 6HIS SUMO LRR1 used