cDNA Clone Ctf4

• Plasmid: pGAD CTF4 (yeast) 461-927 M731A I740A L756A
• Parent Plasmid: pGAD
• Expressed: GalAD-Ctf4(461-927)-3A
• Genbank: NP_015461.1
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

CCATGGCCGGAGGGGCCACTCATTCGTTTCCAATCAGTTTGGCAAACACAGGAAAATTTCGTTATATGCCTTTTTCTCCAGCGGGAACACCTTTTGGCTT
TACTGACAGGCGTTATTTGACAATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACAGCATAACTGTCTCTTTTTTTGATGTTGGA
CGTTTTAGAGAATACCATTTCGAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATTTGGCCAATCCAAAACTGGGCAGATAC
AATATAGGCCACACGATAGCATACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACAAGTGTGGCAGCCACCCCGGTTCGCGT
TATTGTTGGTACATCATTAGGCTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACATCCCCAATTGTAGCGCTTACTGCTCAG
AATTATAGGGTTTTTTCAGTACATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTCTAGTAAAAGGTACTATAAAAGAGAGT
GTCCACTTCCAATGAGTTTACCAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAATTTTAATCCGATGGGCATCAAAAGTTTGTT
CTTTTCAAGCTACGGAGATCCATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGATCACCAGAAGAAAGTAAATGGCTCCCTATT
CTAGATAGCAACATGGAAATATGGAAGGCGTCAGGAGGGAAGGAATCGGCAGATGCACATGTCTGGCCCTTGGCTTTGGCGTATGACACATTGAATTGTA
TCGAAGTTAAGGGCAAGCATATATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCAGTATTTGTTAAGAGTAAATTACTAGA
GGAAAACAAAGCTATATTAAATAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACAAAGAAATACAGATTCCTGTTTCTATG
GCGGCGGAAGAAGAGTATCTGCGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGGTGAAATGTACGGCAACGAAAATGAGGTAT
TGGCAGCATTGAACGGTGCATATGATAAGGCTTTGTTACGTTTATTTGCGTCTGCATGCTCAGACCAAAATGTTGAAAAGGCTCTTTCGCTTGCTCATGA
ATTAAAACAAGATAGAGCACTTACTGCAGCCGTTAAGATATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAAAAATTAATAATATAAGGGAAGCTAGA
TATGAACAGCAATTGAAATGAGGCCACTGGGGCCCCTCGAGTAGCTAGTGTCT

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEILELAMAGGATHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSIT
VSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSP
IVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPE
ESKWLPILDSNMEIWKASGGKESADAHVWPLALAYDTLNCIEVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEIGADTEAEEGEEDKE
IQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVKISERAELPSLVKKI
NNIREARYEQQLK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU51337
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Good morning; I would like a plasmid carrying the fragment Ctf4 (461-927) mutated in three sites (M731A, I740A and L756A). I will provide the initial plasmid (amp resistance) and a DNA strider file with the exact sequence for the final clone.; many thanks; Fabrizio