cDNA Clone Ctf4

• Plasmid: pGAD CTF4 (yeast) 461-927 M731E I740E L756E
• Parent Plasmid: pGAD
• Expressed: GalAD-Ctf4 (461-927)-M731E I740E L756E
• Genbank: NP_015461.1
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

ATTCGTTTCCAATCAGTTTGGCAAACACAGGAAAATTTCGTTATATGCCTTTTTCTCCAGCGGGAACACCTTTTGGCTTTACTGACAGGCGTTATTTGAC
AATGAATGAAGTGGGCTACGTATCTACTGTCAAGAATAGTGAGCAATACAGCATAACTGTCTCTTTTTTTGATGTTGGACGTTTTAGAGAATACCATTTC
GAGGACTTATTTGGCTACGATTTATGCTTCCTAAATGAAAAAGGCACTTTATTTGGCCAATCCAAAACTGGGCAGATACAATATAGGCCACACGATAGCA
TACATTCAAACTGGACCAAGATTATTCCTTTGCAAGCTGGTGAGAGAATAACAAGTGTGGCAGCCACCCCGGTTCGCGTTATTGTTGGTACATCATTAGG
CTATTTCAGAAGTTTCAATCAATTCGGGGTTCCATTTGCTGTTGAAAAGACATCCCCAATTGTAGCGCTTACTGCTCAGAATTATAGGGTTTTTTCAGTA
CATTATTCGCAGTTTCATGGCCTTTCATACTCTTTATCTGAATTGGGTACTTCTAGTAAAAGGTACTATAAAAGAGAGTGTCCACTTCCAATGAGTTTAC
CAAACATTAATTCTGATATGAAAAAAGACGCAAATCTTGACTACTACAATTTTAATCCGATGGGCATCAAAAGTTTGTTCTTTTCAAGCTACGGAGATCC
ATGCATTTTTGGGTCCGACAACACGCTTCTATTGTTATCAAAGTGGAGATCACCAGAAGAAAGTAAATGGCTCCCTATTCTAGATAGCAACATGGAAATA
TGGAAGGAGTCAGGAGGGAAGGAAACGACAGATGAACATGTCTGGCCCTTGGCTTTGGCGTATGACACATTGAATTGTATCGAAGTTAAGGGCAAGCATA
TATGGCCCGAGTTTCCCCTTCCGTTGCCATCCGAAATGGAGATTAGAATGCCAGTATTTGTTAAGAGTAAATTACTAGAGGAAAACAAAGCTATATTAAA
TAAAAAGAACGAAATTGGAGCTGACACCGAAGCGGAAGAAGGGGAAGAAGACAAAGAAATACAGATTCCTGTTTCTATGGCGGCGGAAGAAGAGTATCTG
CGCAGCAAGGTTTTGTCAGAGCTGTTGACAGATACACTCGAAAATGACGGTGAAATGTACGGCAACGAAAATGAGGTATTGGCAGCATTGAACGGTGCAT
ATGATAAGGCTTTGTTACGTTTATTTGCGTCTGCATGCTCAGACCAAAATGTTGAAAAGGCTCTTTCGCTTGCTCATGAATTAAAACAAGATAGAGCACT
TACTGCAGCCGTTAAGATATCAGAAAGAGCTGAGCTGCCCTCTCTCGTTAAAAAAATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAAATGA

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEILELAMAGRATHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSIT
VSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSP
IVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPE
ESKWLPILDSNMEIWKESGGKETTDEHVWPLALAYDTLNCIEVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEIGADTEAEEGEEDKE
IQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVKISERAELPSLVKKI
NNIREARYEQQLK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU51199
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Good morning; I would like a plasmid carrying the fragment Ctf4 (461-927) mutated in three sites (M731E, I740E and L756E). I will provide the initial plasmid (amp resistance) and a DNA strider file with the exact sequence for the final clone.; many thanks; Fabrizio