cDNA Clone USP15

• Plasmid: pcDNAfrtto-Flag-USP15 tv1
• Parent Plasmid: pcDNA5 FRT/TO FLAG1
• Expressed: Flag-USP15 tv1
• Genbank: Gene 9958 isoform 1 = NM_001252078.1
• Tag: FLAG
• Species: Human

Sequence of Insert

gcggccgcTATGGCGGAAGGCGGAGCGGCGGATCTGGACACCCAGCGGTC
TGACATCGCGACGCTGCTCAAAACCTCGCTCCGGAAAGGGGACACCTGGT
ACCTAGTCGATAGTCGCTGGTTCAAACAGTGGAAAAAATATGTTGGCTTT
GACAGTTGGGACAAATACCAGATGGGAGATCAAAATGTGTATCCTGGACC
CATTGATAACTCTGGACTTCTCAAAGATGGTGATGCCCAGTCACTTAAGG
AACACCTTATTGATGAATTGGATTACATACTGTTGCCAACTGAAGGTTGG
AATAAACTTGTCAGCTGGTACACATTGATGGAAGGTCAAGAGCCAATAGC
ACGAAAGGTGGTTGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAG
TATATCTCACAGAATTGAAGCTATGTGAAAATGGAAACATGAATAATGTT
GTAACTCGAAGATTTAGCAAAGCTGACACAATAGATACAATTGAAAAGGA
AATAAGAAAAATCTTCAGTATTCCAGATGAAAAGGAGACCAGATTGTGGA
ACAAATACATGAGTAACACATTTGAACCACTGAATAAACCAGACAGCACC
ATTCAGGATGCTGGTTTATACCAAGGACAGGTATTAGTGATAGAACAGAA
AAATGAAGATGGAACATGGCCAAGGGGTCCTTCTACTCCTAAGTCCCCAG
GTGCATCCAATTTTTCAACTTTACCAAAGATCTCTCCTTCATCTCTATCA
AATAATTATAACAACATGAACAACAGAAATGTGAAAAACTCAAATTACTG
TCTTCCATCATATACCGCTTATAAGAACTATGATTATTCGGAACCTGGAA
GAAACAATGAACAGCCAGGCCTCTGTGGCCTAAGTAACTTGGGAAATACG
TGTTTCATGAACTCAGCTATTCAGTGTTTGAGCAACACACCTCCACTTAC
TGAGTATTTCCTCAATGATAAGTATCAAGAAGAACTGAATTTTGACAATC
CCTTAGGAATGAGAGGTGAAATAGCTAAATCTTATGCCGAACTGATCAAG
CAAATGTGGTCTGGAAAGTTTAGCTACGTCACCCCAAGAGCCTTTAAGAC
ACAGGTAGGACGTTTTGCACCTCAGTTCTCTGGATATCAGCAGCAAGACT
GTCAAGAACTGTTAGCTTTCCTATTAGATGGATTACATGAGGATTTGAAT
AGAATTAGGAAAAAACCATATATACAATTAAAAGATGCAGATGGAAGGCC
AGATAAGGTGGTTGCCGAAGAAGCCTGGGAAAACCATTTAAAACGAAATG
ATTCTATCATAGTAGATATATTTCATGGCCTTTTCAAATCAACTTTAGTT
TGTCCTGAGTGTGCTAAGATTTCAGTAACATTTGATCCTTTTTGTTACTT
GACACTTCCATTGCCCATGAAAAAAGAACGCACCTTGGAAGTTTACTTAG
TTAGAATGGATCCACTTACCAAACCTATGCAGTACAAAGTGGTTGTCCCC
AAAATTGGAAACATATTAGATCTTTGTACAGCATTGTCTGCTTTGTCAGG
AATACCTGCAGATAAGATGATAGTTACTGATATATACAATCATAGATTTC
ACAGAATATTCGCTATGGATGAAAACCTTAGTAGTATTATGGAACGGGAT
GATATTTATGTGTTTGAAATTAACATCAATAGGACAGAAGATACAGAGCA
CGTGATTATTCCTGTTTGCCTAAGAGAAAAATTCAGACACTCGAGTTATA
CCCACCATACTGGTTCTTCACTTTTTGGTCAGCCCTTTCTTATGGCTGTA
CCACGAAACAATACTGAAGACAAACTTTATAATCTCCTGCTCTTGAGAAT
GTGCCGATATGTCAAAATATCTACTGAAACTGAAGAAACTGAAGGATCCC
TACACTGCTGTAAGGACCAAAATATTAATGGGAATGGCCCAAATGGCATA
CATGAAGAAGGCTCACCAAGTGAAATGGAAACAGATGAGCCAGATGATGA
ATCCAGCCAGGATCAAGAACTTCCCTCAGAGAATGAAAACAGTCAGTCTG
AAGATTCAGTTGGAGGAGATAATGATTCTGAAAATGGATTATGTACTGAG
GATACTTGCAAAGGTCAACTCACGGGACACAAAAAACGATTGTTTACATT
CCAGTTCAACAACTTAGGCAATACTGATATCAACTACATCAAAGATGATA
CCAGGCATATAAGATTTGATGATAGGCAGCTTAGGCTAGATGAAAGATCT
TTTCTTGCTTTGGATTGGGATCCTGATTTGAAAAAAAGATATTTTGATGA
AAATGCTGCCGAGGACTTTGAAAAACATGAAAGTGTGGAGTATAAACCTC
CTAAAAAACCCTTTGTGAAATTAAAAGATTGCATTGAACTTTTTACAACA
AAAGAAAAGCTAGGTGCTGAAGATCCCTGGTATTGTCCGAATTGTAAAGA
ACATCAGCAAGCCACAAAGAAATTGGATTTATGGTCCCTGCCTCCAGTAC
TTGTAGTACATCTCAAGCGATTTTCTTACAGTCGATACATGAGAGACAAG
TTGGATACCTTAGTTGATTTTCCTATCAATGACTTGGATATGTCGGAATT
CTTAATTAATCCAAATGCAGGTCCTTGCCGCTATAATCTGATTGCTGTTT
CCAACCACTATGGAGGGATGGGAGGAGGACACTATACTGCTTTTGCAAAA
AATAAAGATGATGGAAAATGGTACTATTTTGATGACAGTAGTGTCTCCAC
TGCATCTGAAGACCAAATTGTGTCCAAAGCAGCATATGTACTCTTCTACC
AGAGACAAGACACTTTCAGTGGAACTGGCTTTTTTCCTCTTGACCGAGAA
ACTAAAGGTGCTTCAGCTGCCACTGGCATCCCATTAGAAAGTGATGAAGA
TAGCAATGATAATGACAATGATATAGAAAATGAAAACTGTATGCACACTA
ACTAAgcggccgc

Amino Acid Sequence

MDYKDDDDKGSPGEFASLEKLAAAMAEGGAADLDTQRSDIATLLKTSLRK
GDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDA
QSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKH
CKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKE
TRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPST
PKSPGASNFSTLPKISPSSLSNNYNNMNNRNVKNSNYCLPSYTAYKNYDY
SEPGRNNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEEL
NFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGY
QQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENH
LKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERTL
EVYLVRMDPLTKPMQYKVVVPKIGNILDLCTALSALSGIPADKMIVTDIY
NHRFHRIFAMDENLSSIMERDDIYVFEININRTEDTEHVIIPVCLREKFR
HSSYTHHTGSSLFGQPFLMAVPRNNTEDKLYNLLLLRMCRYVKISTETEE
TEGSLHCCKDQNINGNGPNGIHEEGSPSEMETDEPDDESSQDQELPSENE
NSQSEDSVGGDNDSENGLCTEDTCKGQLTGHKKRLFTFQFNNLGNTDINY
IKDDTRHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDFEKHESV
EYKPPKKPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWS
LPPVLVVHLKRFSYSRYMRDKLDTLVDFPINDLDMSEFLINPNAGPCRYN
LIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAY
VLFYQRQDTFSGTGFFPLDRETKGASAATGIPLESDEDSNDNDNDIENEN
CMHTN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24863
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: two rounds of insertion mutagenesis on 42486 with NW3444/45 and then NW3539/40 to build full length tv1. Silent mutation at Ala767 compared to NCBI ref