cDNA Clone USP30

• Plasmid: pSCB USP30 Cter GFP donor bam not
• Parent Plasmid: pSC-B
• Genbank: NM_032663.3
• Species: Human

Sequence of Insert

GGATCCGTCCAAAATGGAAATAACTTTTAAATTAAAAATTTTTAAAGTACATAAAATTTACATATGCATATGTATGTGTGTATATATGTGTGTATGTATAAAATATAAAGAAAATTTAAAATTTCTTGGGATACCATCTAGAGATAGCCAATTAAACACTTAGGGTCATGCACTCTATAAAGTTGTACATCACACATACATATAGTGTACATAAACACATACACTCATTGATACAAACATCCACATATACACAATATACATATACTCATAGAATGTACATCTTGCCATGCCAGTAAATACAGATCTGTATTTTAATAGTTGTGTAGCATTTGGTGGTATGGACTTACCATTGTATTCATCCCCTATTTAACATTAGCATTCTTTTGTTTTTGCCTATAAAGTCTTTTTGTTAAAATTGTAAACCCCTGACATGTGTTCGTATCATTCAGCTCCTCCACATACCTCTTCCGGCTGATGGCAGTTGTCGTCCACCATGGAGACATGCACTCTGGACACTTTGTCACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCAACTAGCAATCAGTGGCTGTGGGTCTCCGATGACACTGTCCGCAAGGCCAGCTTACAAGAGGTCTTGAGTTCCAGCGCCTACCTGCTGTTCTACGAGCGCGTCCTTAGTCGTATGCAGCATCAGTCTCAGGAGTGCAAGTCTGAAGAAGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGACTGTGCCCTCCTGCAAGGCTAGAGCTGATGGCACTGTCTGCACTGTCCAGGAAAAAAGTAAAACTGTACTGTTGCGTGTGCAAGCGGCCCCACTAGAGCCTTCCAGCCTTCTGGTGTGTTCTAAGAGCAGGCTCCACCTGGGAGCCAGCCCCAGTTCACACCAAACCAGGCTCCCTGAACAGTCCTGTTCATGTGTGTAGGTGGTTCTGTTGTGTTAAGAAAGCATTCATTATGTCCGGAGTGTCTTTTTACTCATCTGATACAGGTAATTAAAAGAACTCAGATTCTTGAAGCCACCGTTTTCATATTGTAATGTTAGGTGTTCTCAGAGGGGAGGTACCTTTGTCTAATCAACGTTTCCACTTAGATCTTTTATTTTTAATAAGCAGGCCCATAAAAATTGTTGACAAGAATTAATGAAATTATTAAAGGCAACAATTTAGAAGAAAAAGTGCCTTTCACTTTCGATTGCTTTTGTAGCACGTCCATTGTGAAATATTCCTTCCAGGCTACTCAAAGGATAGCAAGAGAACAGGTAAATGATGCCTAAAGAACACCGCGGCCGC

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48985
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor plasmid. Designed to KI a GFP reporter to the c-terminus of USP30 when co-transfected with guide pairs 48939/48953 or 48939/48954. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary. Note this donor requires modification to protect from guides.