cDNA Clone Ubiquitin

• Plasmid: pGEX6P1 Cys Ubiquitin F4A
• Parent Plasmid: pGEX6P1
• Expressed: GST-Cys-Ubiquitin F4A
• Genbank: P62987.2 GI:302393718
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCAGATGCAAATCGCCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLCGSQMQIAVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL
HLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50345
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has extra 'QM' residues before Q2 of Ubiquitin compared with related clones (DU50027, DU50042, DU50330, DU50331, DU50346, DU50465, DU50466)