cDNA Clone USP24

• Plasmid: pBABED P U6 USP24 KO sense
• Parent Plasmid: pBABED P U6
• Expressed: USP24 KO sense
• Genbank: NM_015306.2
• Species: Human

Sequence of Insert

GACCGGATCTGGTGACAATTG

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48890
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets sense strand in exon 10, designed to KO USP24 when co-transfected with antisense guide 52013. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty flp-in line is preferable as this allows for downstream rescue and mutant analysis if necessary.