cDNA Clone WNK1

• Synonyms: HSAN2, HSN2, KDP, PPP1R167, PRKWNK1, PSK, p65
• Plasmid: pX335 WNK1 D368A antisense.1
• Parent Plasmid: pX335
• Expressed: WNK1 D368A antisense.1 guide
• Genbank: NM_018979.3
• Species: Human

Sequence of Insert

GCAATCTTGACTGAGCCA

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48783
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets exon 3, designed to introduce a D368A point mutation when used in conjunction with sense guide 48774 and a ssODN donor (D10A approach). Guide sequence shown as sequence of insert and donor shown as expression cassette. Positives may be enriched for with puromycin prior to further selection using western blotting with a phospho specific antibody or RFLP using the SfoI site introduced upon recombination.