cDNA Clone WNK1

• Synonyms: HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1; PSK
• Plasmid: pTREG mod FLAG WNK1 isoform 1
• Parent Plasmid: pTREG Mod
• Expressed: FLAG WNK1 isoform 1
• Genbank: NM_018979.3 GI:296939601 with changes - see notes
• Tag: FLAG
• Species: Human

Sequence of Insert

gTCGACATGGACTACAAGGACGACGATGACAAGTCTGGCGGCGCCGCAGA
GAAGCAGAGCAGCACTCCCGGTTCCCTGTTCCTCTCGCCGCCGGCTCCTG
CCCCCAAGAATGGCTCCAGCTCCGATTCCTCCGTGGGGGAGAAACTGGGA
GCCGCGGCCGCCGACGCTGTGACCGGCAGGACCGAGGAGTACAGGCGCCG
CCGCCACACTATGGACAAGGACAGCCGTGGGGCGGCCGCGACCACTACCA
CCACTGAGCACCGCTTCTTCCGCCGGAGCGTCATCTGCGACTCCAATGCC
ACTGCACTGGAGCTTCCCGGCCTTCCTCTTTCCCTGCCCCAGCCCAGCAT
CCCCGCGGCTGTCCCGCAGAGTGCTCCACCGGAGCCCCACCGGGAAGAGA
CCGTGACCGCCACCGCCACTTCCCAGGTAGCCCAGCAGCCTCCAGCCGCT
GCCGCCCCTGGGGAACAGGCCGTCGCGGGCCCTGCCCCCTCGACTGTCCC
CAGCAGTACCAGCAAAGACCGCCCAGTGTCCCAGCCTAGCCTTGTGGGGA
GCAAAGAGGAGCCGCCGCCGGCGAGAAGTGGCAGCGGCGGCGGCAGCGCC
AAGGAGCCACAGGAGGAACGGAGCCAGCAGCAGGATGATATCGAAGAGCT
GGAGACCAAGGCCGTGGGAATGTCTAACGATGGCCGCTTTCTCAAGTTTG
ACATCGAAATCGGCAGAGGCTCCTTTAAGACGGTCTACAAAGGTCTGGAC
ACTGAAACCACCGTGGAAGTCGCCTGGTGTGAACTGCAGGATCGAAAATT
AACAAAGTCTGAGAGGCAGAGATTTAAAGAAGAAGCTGAAATGTTAAAAG
GTCTTCAGCATCCCAATATTGTTAGATTTTATGATTCCTGGGAATCCACA
GTAAAAGGAAAGAAGTGCATTGTTTTGGTGACTGAACTTATGACGTCTGG
AACACTTAAAACGTATCTGAAAAGGTTTAAAGTGATGAAGATCAAAGTTC
TAAGAAGCTGGTGCCGTCAGATCCTTAAAGGTCTTCAGTTTCTTCATACT
CGAACTCCACCTATCATTCACCGCGATCTTAAATGTGACAACATCTTTAT
CACCGGCCCTACTGGCTCAGTCAAGATTGGAGACCTCGGTCTGGCAACCC
TGAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTCATG
GCCCCTGAGATGTATGAGGAGAAATATGATGAATCCGTTGACGTTTATGC
TTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATATCCTTACTCGG
AGTGCCAAAATGCTGCGCAGATCTACCGTCGCGTGACCAGTGGGGTGAAG
CCAGCCAGTTTTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATTATTGA
AGGATGCATACGACAAAACAAAGATGAAAGATATTCCATCAAAGACCTTT
TGAACCATGCCTTCTTCCAAGAGGAAACAGGAGTACGGGTAGAATTAGCA
GAAGAAGATGATGGAGAAAAAATAGCCATAAAATTATGGCTACGTATTGA
AGATATTAAGAAATTAAAGGGAAAATACAAAGATAATGAAGCTATTGAGT
TTTCTTTTGATTTAGAGAGAGATGTCCCAGAAGATGTTGCACAAGAAATG
GTAGAGTCTGGGTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGC
TATCAAAGACAGAGTATCATTAATTAAGAGGAAACGAGAGCAGCGGCAGT
TGGTACGGGAGGAGCAAGAAAAAAAAAAGCAGGAAGAGAGCAGTCTCAAA
CAGCAGGTAGAACAATCCAGTGCTTCCCAGACAGGAATCAAGCAGCTCCC
TTCTGCTAGCACCGGCATACCTACTGCTTCTACCACTTCAGCTTCAGTTT
CTACACAAGTAGAACCTGAAGAACCTGAGGCAGATCAACATCAGCAACTA
CAGTACCAGCAACCCAGTATATCTGTGTTATCTGATGGGACGGTTGACAG
TGGTCAGGGATCCTCTGTCTTCACAGAATCTCGAGTGAGCAGCCAACAGA
CAGTTTCATATGGTTCCCAACATGAACAGGCACATTCTACAGGCACAGTC
CCAGGGCATATACCTTCTACTGTCCAAGCACAGTCTCAGCCCCATGGGGT
ATATCCACCCTCAAGTGTGGCACAGGGGCAGAGCCAGGGTCAGCCATCCT
CAAGTAGCTTAACAGGGGTTTCATCTTCCCAACCCATACAACATCCTCAG
CAGCAGCAGGGAATACAGCAGACAGCCCCTCCTCAACAGACAGTGCAGTA
TTCACTTTCACAGACATCAACCTCCAGTGAGGCCACTACTGCACAGCCAG
TGAGTCAACCTCAAGCTCCACAAGTCTTGCCTCAAGTATCAGCTGGAAAA
CAGCTTCCAGTTTCCCAGCCAGTACCAACTATCCAAGGCGAACCTCAGAT
CCCAGTTGCGACACAACCCTCGGTTGTTCCAGTCCACTCTGGTGCTCATT
TCCTTCCAGTGGGACAGCCGCTCCCTACTCCCTTGCTCCCTCAGTACCCT
GTCTCTCAGATTCCCATATCAACTCCTCATGTGTCTACGGCTCAGACAGG
TTTCTCATCCCTTCCCATCACAATGGCAGCTGGCATTACTCAGCCTCTGC
TCACGTTGGCTTCATCTGCTACAACAGCTGCGATCCCGGGGGTATCAACT
GTGGTTCCTAGTCAGCTTCCAACCCTTCTGCAGCCTGTGACTCAGCTGCC
AAGTCAGGTTCACCCACAGCTCCTACAACCAGCAGTTCAGTCCATGGGAA
TACCAGCTAACCTTGGACAAGCTGCTGAGGTTCCACTTTCCTCTGGAGAT
GTTCTGTACCAGGGCTTCCCACCTCGACTGCCACCACAGTACCCAGGAGA
TTCAAATATTGCTCCCTCTTCCAACGTGGCTTCTGTTTGCATCCATTCTA
CAGTCCTATCCCCTCCCATGCCGACAGAAGTACTGGCTACACCTGGGTAC
TTTCCCACAGTGGTGCAGCCTTATGTGGAATCAAATCTTTTAGTTCCTAT
GGGTGGTGTAGGAGGACAGGTTCAAGTGTCCCAGCCAGGAGGGAGTTTAG
CACAAGCCCCCACTACATCCTCCCAGCAAGCAGTTTTGGAGAGTACTCAG
GGAGTCTCTCAGGTTGCTCCTGCAGAGCCAGTTGCAGTAGCACAGCCCCA
AGCTACCCAGCCGACCACTTTGGCTTCCTCTGTAGACAGTGCACATTCAG
ATGTTGCTTCAGGTATGAGTGATGGCAATGAGAACGTCCCATCTTCCAGT
GGAAGGCATGAAGGAAGAACTACAAAACGGCATTACCGAAAATCTGTAAG
GAGTCGCTCTCGACATGAAAAAACTTCACGCCCAAAATTAAGAATTTTGA
ATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGACTCAT
AATAGGAAAATGGTTACATTCAAATTTGACCTAGATGGTGACAACCCCGA
GGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCAATAGAGA
GAGAGTCGTTTGTGGATCAAGTGCGAGAAATTATTGAAAAAGCTGATGAA
ATGCTCAGTGAGGATGTCAGTGTGGAACCAGAGGGTGATCAGGGATTGGA
GAGTCTACAAGGAAAGGATGACTATGGCTTTTCAGGTTCTCAGAAGCTTG
AAGGAGAGTTCAAACAACCAATTCCTGCGTCTTCCATGCCACAGCAAATA
GGCATTCCTACCAGTTCTTTAACTCAAGTTGTTCATTCTGCGGGAAGGCG
GTTTATAGTGAGTCCTGTGCCAGAAAGCCGATTACGAGAATCAAAAGTTT
TCCCCAGTGAAATAACAGATACAGTTGCTGCCTCTACAGCTCAGAGCCCT
GGAATGAACTTGTCTCACTCTGCATCATCCCTTAGTCTACAACAGGCCTT
TTCTGAACTTAGACGTGCCCAAATGACAGAAGGACCCAACACAGCACCTC
CAAACTTTAGTCATACAGGACCAACATTTCCAGTAGTACCTCCTTTCTTA
AGTAGCATTGCTGGAGTCCCAACCACAGCAGCAGCCACAGCACCAGTCCC
TGCAACAAGCAGCCCTCCTAATGACATTTCCACATCAGTAATTCAGTCTG
AGGTTACAGTGCCCACTGAAGAGGGGATTGCTGGAGTTGCCACCAGCACA
GGTGTGGTAACTTCGGGTGGTCTCCCCATACCACCTGTGTCTGAATCACC
AGTACTTTCCAGCGTAGTTTCAAGTATCACAATACCTGCAGTTGTCTCAA
TATCTACTACATCCCCGTCACTTCAAGTCCCCACATCCACATCTGAGATC
GTTGTTTCTAGTACAGCACTGTATCCTTCAGTAACAGTTTCAGCAACTTC
AGCCTCTGCAGGGGGCAGTACTGCTACCCCAGGTCCTAAGCCTCCAGCTG
TAGTATCTCAGCAGGCAGCAGGCAGCACTACTGTGGGAGCCACATTAACA
TCAGTTTCTACCACCACTTCATTCCCAAGCACAGCTTCACAGCTGTCCAT
TCAGCTTAGCAGCAGTACTTCTACTCCTACTTTAGCTGAAACCGTGGTAG
TTAGCGCACACTCACTAGATAAGACATCTCATAGCAGTACAACTGGATTG
GCTTTCTCCCTCTCTGCACCATCTTCCTCTTCCTCTCCTGGAGCAGGAGT
GTCTAGTTATATTTCTCAGCCTGGTGGGCTGCATCCTTTGGTCATTCCAT
CAGTGATAGCTTCTACTCCTATTCTTCCCCAAGCAGCAGGACCTACTTCT
ACACCTTTATTACCCCAAGTACCTAGTATCCCACCCTTGGTACAGCCTGT
TGCCAATGTGCCTGCTGTACAGCAGACACTAATTCATAGTCAGCCTCAAC
CAGCTTTGCTTCCCAACCAGCCCCATACTCATTGTCCTGAAGTAGATTCT
GATACACAACCCAAAGCTCCTGGAATTGATGACATAAAGACTCTAGAAGA
AAAGCTGCGGTCTCTGTTCAGTGAACACAGCTCATCTGGAGCTCAGCATG
CCTCTGTCTCACTGGAGACCTCACTAGTCATAGAGAGCACTGTCACACCA
GGCATCCCAACTACTGCTGTTGCACCAAGCAAACTCCTGACTTCTACCAC
AAGTACTTGCTTACCACCAACCAATTTACCACTAGGAACAGTTGCTTTGC
CAGTTACACCAGTGGTCACACCTGGGCAAGTTTCTACCCCAGTCAGCACT
ACTACATCAGGAGTGAAACCTGGAACTGCTCCCTCCAAGCCACCTCTAAC
TAAGGCTCCGGTGCTGCCAGTGGGTACTGAACTTCCAGCAGGTACTCTAC
CCAGCGAGCAGCTGCCACCTTTTCCAGGACCTTCTCTAACCCAGTCCCAG
CAACCTCTAGAGGATCTTGATGCTCAATTGAGAAGAACACTTAGTCCAGA
GATTATCACAGTGACTTCTGCGGTTGGTCCTGTGTCCATGGCGGCTCCAA
CAGCAATCACAGAAGCAGGAACACAGCCTCAGAAGGGTGTTTCTCAAGTC
AAAGAAGGCCCTGTCCTAGCGACTAGTTCAGGAGCTGGTGTTTTTAAGAT
GGGACGATTTCAGGTTTCTGTTGCAGCAGACGGTGCCCAGAAAGAGGGTA
AAAATAAGTCAGAAGATGCAAAGTCTGTTCATTTTGAATCCAGCACCTCA
GAGTCCTCAGTGCTATCAAGTAGTAGTCCAGAGAGTACCTTGGTGAAACC
AGAGCCGAATGGCATAACCATCCCTGGTATCTCTTCAGATGTGCCAGAGA
GTGCCCACAAAACTACTGCCTCAGAGGCAAAGTCAGACACTGGGCAGCCT
ACCAAGGTTGGACGTTTTCAGGTGACAACTACAGCAAACAAAGTGGGTCG
TTTCTCTGTATCAAAAACTGAGGACAAGATCACTGACACAAAGAAAGAAG
GACCAGTGGCATCTCCTCCTTTTATGGATTTGGAACAAGCTGTTCTTCCT
GCTGTGATACCAAAGAAAGAGAAGCCTGAACTGTCAGAGCCTTCACATCT
AAATGGGCCGTCTTCTGACCCGGAGGCCGCTTTTTTAAGTAGGGATGTGG
ATGATGGTTCCGGTAGTCCACACTCGCCCCATCAGCTGAGCTCAAAGAGC
CTTCCTAGCCAGAATCTAAGTCAAAGCCTTAGTAATTCATTTAACTCCTC
TTACATGAGTAGCGACAATGAGTCAGATATCGAAGATGAAGACTTAAAGT
TAGAGCTGCGACGACTACGAGATAAACATCTCAAAGAGATTCAGGACCTG
CAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACCAAACTGGGCAA
GGTGCCCCCTGCTGTTATTATTCCCCCAGCTGCTCCCCTTTCAGGGAGAA
GACGACGACCCACTAAAAGCAAAGGCAGCAAATCTAGTCGAAGCAGTTCC
TTGGGGAATAAAAGCCCCCAGCTTTCAGGTAACCTGTCTGGTCAGAGTGC
AGCTTCAGTCTTGCACCCCCAGCAGACCCTCCACCCTCCTGGCAACATCC
CAGAGTCCGGGCAGAATCAGCTGTTACAGCCCCTTAAGCCATCTCCCTCC
AGTGACAACCTCTATTCAGCCTTCACCAGTGATGGTGCCATTTCAGTACC
AAGCCTTTCTGCTCCAGGTCAAGGAACCAGCAGCACAAACACTGTTGGGG
CAACAGTGAACAGCCAAGCCGCCCAAGCTCAGCCTCCTGCCATGACGTCC
AGCAGGAAGGGCACATTCACAGATGACTTGCACAAGTTGGTAGACAATTG
GGCCCGAGATGCCATGAATCTCTCAGGCAGGAGAGGAAGCAAAGGGCACA
TGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCACCTGGGCAA
CTGTGCATCTCCATGACCTCGAACCTGGGTGGCTCTGCCCCCATCTCTGC
AGCATCAGCTACCTCTCTAGGTCACTTCACCAAGTCTATGTGCCCCCCAC
AGCAGTATGGCTTTCCAGCTACCCCATTTGGCGCTCAATGGAGTGGGACG
GGTGGCCCAGCACCACAGCCACTTGGCCAGTTCCAACCTGTGGGAACTGC
CTCCTTGCAGAATTTCAACATCAGCAATTTGCAGAAATCCATCAGCAACC
CCCCAGGCTCCAACCTGCGGACCACTTAGTCGAc

Amino Acid Sequence

MDYKDDDDKSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAA
AADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATA
LELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAA
PGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKE
PQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTE
TTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVK
GKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRT
PPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAP
EMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPA
SFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEE
DDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVE
SGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQ
VEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQY
QQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPG
HIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQ
QGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQL
PVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVS
QIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVV
PSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVL
YQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFP
TVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGV
SQVAPAEPVAVAQPQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGR
HEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNR
KMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEML
SEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGI
PTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGM
NLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSS
IAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGV
VTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVV
SSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSV
STTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAF
SLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTP
LLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDT
QPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGI
PTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTT
SGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQP
LEDLDAQLRRTLSPEIITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKE
GPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSES
SVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTK
VGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAV
IPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLP
SQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQS
RQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLG
NKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSD
NLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSR
KGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLC
ISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGG
PAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU50138
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Drosophila expression construct.; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Silent changes compared with NM_018979.3 CDS:; ; t258c, a1287g, a1914g, g2328a, a3666g, t3667c, g3669t, a4185g, a5541g, c6828t.; ; Coding changes compared with NM_018979.3 CDS:; ; a3166c (T1056P); g4517c (C1506S); g5424t (M1808I); All three seen in other published sequences for WNK1