cDNA Clone USP25

• Plasmid: pSc-B-USP25 (no stop) C178A
• Parent Plasmid: pSC-B
• Genbank: NP_037528.3
• Species: Human

Sequence of Insert

GGATCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAA
GCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATTACGGGGATTAATG
ACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAA
TTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGA
GGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCA
GTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGAT
AAAGATGATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAG
GGCATTCAGGGAGACTGGAATAACTGATGAGGAACAAGCCATTAGCAGAG
TTCTTGAAGCCAGCATAGCAGAGAATAAAGCATGTTTGAAGAGGACACCT
ACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGACAGGA
CAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTgcTTGGTTTAGTG
CTGTTATTCAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTG
AATTACAAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGA
ACATCGGAATTTGCCTTTTATGCGTGAGCTGAGGTATCTATTTGCACTTC
TTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATT
CTTAAGGATGCTTTCAAATCAAATGACTCACAGCAGCAAGATGTGAGTGA
GTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATGAAAG
CTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTG
TTCTATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGA
AAACACTGAAATGTTTGGTCAGTACCCACTTCAGGTCAATGGGTTCAAAG
ATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAAGGAGAAATTGAGTCT
TTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGA
ATTACCACCTGTGTTAACATTTGAATTGTCAAGATTTGAATTTAATCAGG
CATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTT
TTATATTTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAA
GAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAA
GGCTAGAAAGATATTTAAGCTATGGTTCCGGTCCCAAACGATTCCCCTTG
GTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCAAGTAAACCTGTTTG
CACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCA
TACCATCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCT
TCAGAACTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAG
ATCAGTAATACACAAACCATTTACTCAGTCCCGGATACCTCCAGATTTGC
CCATGCATCCGGCACCGAGGCACATAACGGAGGAAGAACTTTCTGTGCTG
GAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAAAATGACACCAGAGA
TTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATGTACT
CTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTT
CACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCA
TCGTGAAAGCAGATGGATGAAGTACAATGATATTGCTGTGACAAAATCAT
CATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGTTATAGAAATGCCAGT
GCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGA
GGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACATTAC
CACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAA
GAACTAGAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAA
GCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAG
CACAAGCAGCAGGAGACCCAGAATATCTAGAGCAGCCATCAAGAAGTGAT
TTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATAATTACCAAGGCATC
ACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCAATTA
AGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCA
GAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCA
GGCACCAAAGAAAATTATTGAGAAAACATTACTAGAACAATTTGGAGATA
GAAATTTGAGTTTTGATGAAAGGTGTCACAACATAATGAAAGTTGCTCAA
GCCAAACTGGAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAATATGA
GGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTCA
TAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTG
TTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTT
ATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTT
TGCTAAAATTAAATGAGCAAGCCGCAGAACTCTTCGAATCTGGAGAGGAT
CGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTCCCATT
TTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTAGCTG
TAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAA
CCACACCTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTG
TTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAAGTTACCTTCATATT
CCACGCATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGT
CGAACTCCTGCTGATGGAAGAGCGGCCGC

Amino Acid Sequence

MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELA
VAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKD
DLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTE
VWRDSRNPYDRKRQDKAPVGLKNVGNTAWFSAVIQSLFNLLEFRRLVLNY
KPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILK
DAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFY
GRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLH
SENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLY
LDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVD
VLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSE
LPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLES
CLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHE
GQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAY
CLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKEL
EEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFS
KHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPET
DYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAK
LEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFL
ICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDRE
VNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPH
LQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRT
PADGR

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU24716
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Harbours a1611g (silent at Pro537) compared to ref sequence