cDNA Clone USP47

• Synonyms: TRFP
• Plasmid: pFB HTb USP47 tv6 C109A (C177A)
• Parent Plasmid: pFastBac HTb
• Expressed: 6HIS-USP47 tv6 C109A (C177A)
• Genbank: Q96K76
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGACCGAAAACCTGTATTTTCAGGGCGCCATGGGATCCGGAATTCAAAGGCCTACGTCGACGAGCTCACTAGTCGCGGCCGCCATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAAGAGATAGAAAATGCTGCTGAAGAACCTAGAGTCTTATGTATTATACAAGATACTACTAATTCAAAGACAGTGAATGAACGGATCACTTTAAATTTACCAGCATCTACTCCAGTCAGAAAGCTCTTTGAAGATGTGGCCAACAAAGTAGGCTACATAAATGGAACCTTTGACTTGGTGTGGGGAAATGGAATCAATACTGCTGATATGGCACCACTGGATCATACCAGTGACAAGTCACTTCTCGACGCTAATTTTGAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACAACCTCAAATACTGCTGGAGGATTCCAGTGCTGGGGAAGACAGTGTTCATGACAGGTTTATAGGTCCGCTTCCAAGAGAAGGTTCTGGGGGTTCTACCAGTGATTATGTCAGCCAAAGCTACTCCTACTCATCTATTTTGAATAAATCAGAAACTGGATATGTGGGACTAGTAAACCAAGCAATGACTGCCTATTTGAATAGCCTTTTGCAAACACTTTTTATGACTCCTGAATTTAGGAATGCATTATATAAGTGGGAATTTGAAGAATCTGAAGAAGATCCAGTGACAAGTATTCCATACCAACTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAATTGAAACCACAGATGTTACAAGGAGCTTTGGATGGGATAGTAGTGAGGCTTGGCAGCAGCATGATGTACAAGAACTATGCAGAGTCATGTTTGATGCTTTGGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAATGAGCTATATCAAGGCAAGCTGAAGGACTACGTGAGATGTCTGGAATGTGGTTATGAGGGCTGGCGAATCGACACATATCTTGATATTCCATTGGTCATCCGACCTTATGGGTCCAGCCAAGCATTTGCTAGTGTGGAAGAAGCATTGCATGCATTTATTCAGCCAGAGATTCTGGATGGCCCAAATCAGTATTTTTGTGAACGTTGTAAGAAGAAGTGTGATGCACGGAAGGGCCTTCGGTTTTTGCATTTTCCTTATCTGCTGACCTTACAGCTGAAAAGATTCGATTTTGATTATACAACAATGCATAGGATTAAACTGAATGATCGAATGACATTTCCCGAGGAACTAGATATGAGTACTTTTATTGATGTTGAAGATGAGAAATCTCCTCAGACTGAAAGTTGCACTGACAGTGGAGCAGAAAATGAAGGTAGTTGTCACAGTGATCAGATGAGCAACGATTTCTCCAATGATGATGGTGTTGATGAAGGAATCTGTCTTGAAACCAATAGTGGAACTGAAAAGATCTCAAAATCTGGACTTGAAAAGAATTCCTTGATCTATGAACTTTTCTCTGTTATGGCTCATTCTGGGAGCGCTGCTGGTGGTCATTATTATGCATGTATAAAGTCATTCAGTGATGAGCAGTGGTACAGCTTCGATGATCAACATGTCAGCAGGATAACACAAGAGGACATTAAGAAAACACATGGTGGATCTTCAGGAAGCAGAGGATATTATTCTAGTGCTTTCGCAAGTTCCACAAATGCATATATGCTGATCTATAGACTGAAGGATCCAGCCAGAAATGCAAAATTTCTAGAAGTGGATGAATACCCAGAACATATTAAAAACTTGGTGCAGAAAGAGAGAGAGTTGGAAGAACAAGAAAAGAGACAACGAGAAATTGAGCGCAATACATGCAAGATAAAATTATTCTGTTTGCATCCTACAAAACAAGTAATGATGGAAAATAAATTGGAGGTTCATAAGGATAAGACATTAAAGGAAGCAGTAGAAATGGCTTATAAGATGATGGATTTAGAAGAGGTAATACCCCTGGATTGCTGTCGCCTTGTTAAATATGATGAGTTTCATGATTATCTAGAACGGTCATATGAAGGAGAAGAAGATACACCAATGGGGCTTCTACTAGGTGGCGTCAAGTCAACATATATGTTTGATCTGCTGTTGGAGACGAGAAAGCCTGATCAGGTTTTCCAATCTTATAAACCTGGAGAAGTGATGGTGAAAGTTCATGTTGTTGATCTAAAGGCAGAATCTGTAGCTGCTCCTATAACTGTTCGTGCTTACTTAAATCAGACAGTTACAGAATTCAAACAACTGATTTCAAAGGCCATCCATTTACCTGCTGAAACAATGAGAATAGTGCTGGAACGCTGCTACAATGATTTGCGTCTTCTCAGTGTCTCCAGTAAAACCCTGAAAGCTGAAGGATTTTTTAGAAGTAACAAGGTGTTTGTTGAAAGCTCCGAGACTTTGGATTACCAGATGGCCTTTGCAGACTCTCATTTATGGAAACTCCTGGATCGGCATGCAAATACAATCAGATTATTTGTTTTGCTACCTGAACAATCCCCAGTATCTTATTCCAAAAGGACAGCATACCAGAAAGCTGGAGGCGATTCTGGTAATGTGGATGATGACTGTGAAAGAGTCAAAGGACCTGTAGGAAGCCTAAAGTCTGTGGAAGCTATTCTAGAAGAAAGCACTGAAAAACTCAAAAGCTTGTCACTGCAGCAACAGCAGGATGGAGATAATGGGGACAGCAGCAAAAGTACTGAGACAAGTGACTTTGAAAACATCGAATCACCTCTCAATGAGAGGGACTCTTCAGCATCAGTGGATAATAGAGAACTTGAACAGCATATTCAGACTTCTGATCCAGAAAATTTTCAGTCTGAAGAACGATCAGACTCAGATGTGAATAATGACAGGAGTACAAGTTCAGTGGACAGTGATATTCTTAGCTCCAGTCATAGCAGTGATACTTTGTGCAATGCAGACAATGCTCAGATCCCTTTGGCTAATGGACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACGAAAGCAAATGAAGGGAAAAAAGAAACATGGGATACAGCAGAAGAAGACTCTGGAACTGATAGTGAATATGATGAGAGTGGCAAGAGTAGGGGAGAAATGCAGTACATGTATTTCAAAGCTGAACCTTATGCTGCAGATGAAGGTTCTGGGGAAGGACATAAATGGTTGATGGTGCATGTTGATAAAAGAATTACTCTGGCAGCTTTCAAACAACATTTAGAGCCCTTTGTTGGAGTTTTGTCCTCTCACTTCAAGGTCTTTCGAGTGTATGCCAGCAATCAAGAGTTTGAGAGCGTCCGGCTGAATGAGACACTTTCATCATTTTCTGATGACAATAAGATTACAATTAGACTGGGGAGAGCACTTAAAAAAGGAGAATACAGAGTTAAAGTATACCAGCTTTTGGTCAATGAACAAGAGCCATGCAAGTTTCTGCTAGATGCTGTGTTTGCTAAAGGAATGACTGTACGGCAATCAAAAGAGGAATTAATTCCTCAGCTCAGGGAGCAATGTGGTTTAGAGCTCAGTATTGACAGGTTTCGTCTAAGGAAAAAAACATGGAAGAATCCTGGCACTGTCTTTTTGGATTATCATATTTATGAAGAAGATATTAATATTTCCAGCAACTGGGAGGTTTTCCTTGAAGTTCTTGATGGGGTAGAGAAGATGAAGTCCATGTCACAGCTTGCAGTTTTGTCAAGACGGTGGAAGCCTTCAGAGATGAAGTTGGATCCCTTCCAGGAGGTTGTATTGGAAAGCAGTAGTGTGGACGAATTGCGAGAGAAGCTTAGTGAAATCAGTGGGATTCCTTTGGATGATATTGAATTTGCTAAGGGTAGAGGAACATTTCCCTGTGATATTTCTGTCCTTGATATTCATCAAGATTTAGACTGGAATCCTAAAGTTTCTACGCTGAATGTCTGGCCTCTTTATATCTGTGATGATGGTGCGGTCATATTTTATAGGGATAAAACAGAAGAATTAATGGAACTGACAGATGAGCAAAGAAATGAACTGATGAAAAAAGAAAGCAGTCGACTCCAGAAGACTGGACATCGTGTAACATACTCACCTCGTAAAGAGAAAGCACTAAAAATATATCTGGATGGAGCACCAAATAAAGATCTGACTCAAGACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAAMVPGEEN
QLVPKEIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPVRKLFEDV
ANKVGYINGTFDLVWGNGINTADMAPLDHTSDKSLLDANFEPGKKNFLHL
TDKDGEQPQILLEDSSAGEDSVHDRFIGPLPREGSGGSTSDYVSQSYSYS
SILNKSETGYVGLVNQAMTAYLNSLLQTLFMTPEFRNALYKWEFEESEED
PVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELC
RVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDI
PLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGL
RFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDEK
SPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLETNSGTEKISK
SGLEKNSLIYELFSVMAHSGSAAGGHYYACIKSFSDEQWYSFDDQHVSRI
TQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPARNAKFLEVDE
YPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPTKQVMMENKLE
VHKDKTLKEAVEMAYKMMDLEEVIPLDCCRLVKYDEFHDYLERSYEGEED
TPMGLLLGGVKSTYMFDLLLETRKPDQVFQSYKPGEVMVKVHVVDLKAES
VAAPITVRAYLNQTVTEFKQLISKAIHLPAETMRIVLERCYNDLRLLSVS
SKTLKAEGFFRSNKVFVESSETLDYQMAFADSHLWKLLDRHANTIRLFVL
LPEQSPVSYSKRTAYQKAGGDSGNVDDDCERVKGPVGSLKSVEAILEEST
EKLKSLSLQQQQDGDNGDSSKSTETSDFENIESPLNERDSSASVDNRELE
QHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADNA
QIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRG
EMQYMYFKAEPYAADEGSGEGHKWLMVHVDKRITLAAFKQHLEPFVGVLS
SHFKVFRVYASNQEFESVRLNETLSSFSDDNKITIRLGRALKKGEYRVKV
YQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLELSIDRFR
LRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAV
LSRRWKPSEMKLDPFQEVVLESSSVDELREKLSEISGIPLDDIEFAKGRG
TFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELMEL
TDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGAPNKDLTQD*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU48861
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Variant 1, C177A (equivalent to C109A in short variant).