cDNA Clone USP15

• Plasmid: pCMV5 FLAG1 USP15
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-USP15
• Genbank: NM_006313.2 GI:355330277 with silent change t2214c
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCTCATGGCGGAAGGCGGAGCGGCGGATCTGGACACCCAGCGGTCTGACATCGCGACGCTGCTCAAAACCTCGCTCCGGAAAGGGGACACCTGGTACCTAGTCGATAGTCGCTGGTTCAAACAGTGGAAAAAATATGTTGGCTTTGACAGTTGGGACAAATACCAGATGGGAGATCAAAATGTGTATCCTGGACCCATTGATAACTCTGGACTTCTCAAAGATGGTGATGCCCAGTCACTTAAGGAACACCTTATTGATGAATTGGATTACATACTGTTGCCAACTGAAGGTTGGAATAAACTTGTCAGCTGGTACACATTGATGGAAGGTCAAGAGCCAATAGCACGAAAGGTGGTTGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTATATCTCACAGAATTGAAGCTATGTGAAAATGGAAACATGAATAATGTTGTAACTCGAAGATTTAGCAAAGCTGACACAATAGATACAATTGAAAAGGAAATAAGAAAAATCTTCAGTATTCCAGATGAAAAGGAGACCAGATTGTGGAACAAATACATGAGTAACACATTTGAACCACTGAATAAACCAGACAGCACCATTCAGGATGCTGGTTTATACCAAGGACAGGTATTAGTGATAGAACAGAAAAATGAAGATGGAACATGGCCAAGGGGTCCTTCTACTCCTAATGTGAAAAACTCAAATTACTGTCTTCCATCATATACCGCTTATAAGAACTATGATTATTCGGAACCTGGAAGAAACAATGAACAGCCAGGCCTCTGTGGCCTAAGTAACTTGGGAAATACGTGTTTCATGAACTCAGCTATTCAGTGTTTGAGCAACACACCTCCACTTACTGAGTATTTCCTCAATGATAAGTATCAAGAAGAACTGAATTTTGACAATCCCTTAGGAATGAGAGGTGAAATAGCTAAATCTTATGCCGAACTGATCAAGCAAATGTGGTCTGGAAAGTTTAGCTACGTCACCCCAAGAGCCTTTAAGACACAGGTAGGACGTTTTGCACCTCAGTTCTCTGGATATCAGCAGCAAGACTGTCAAGAACTGTTAGCTTTCCTATTAGATGGATTACATGAGGATTTGAATAGAATTAGGAAAAAACCATATATACAATTAAAAGATGCAGATGGAAGGCCAGATAAGGTGGTTGCCGAAGAAGCCTGGGAAAACCATTTAAAACGAAATGATTCTATCATAGTAGATATATTTCATGGCCTTTTCAAATCAACTTTAGTTTGTCCTGAGTGTGCTAAGATTTCAGTAACATTTGATCCTTTTTGTTACTTGACACTTCCATTGCCCATGAAAAAAGAACGCACCTTGGAAGTTTACTTAGTTAGAATGGATCCACTTACCAAACCTATGCAGTACAAAGTGGTTGTCCCCAAAATTGGAAACATATTAGATCTTTGTACAGCATTGTCTGCTTTGTCAGGAATACCTGCAGATAAGATGATAGTTACTGATATATACAATCATAGATTTCACAGAATATTCGCTATGGATGAAAACCTTAGTAGTATTATGGAACGGGATGATATTTATGTGTTTGAAATTAACATCAATAGGACAGAAGATACAGAGCACGTGATTATTCCTGTTTGCCTAAGAGAAAAATTCAGACACTCGAGTTATACCCACCATACTGGTTCTTCACTTTTTGGTCAGCCCTTTCTTATGGCTGTACCACGAAACAATACTGAAGACAAACTTTATAATCTCCTGCTCTTGAGAATGTGCCGATATGTCAAAATATCTACTGAAACTGAAGAAACTGAAGGATCCCTACACTGCTGTAAGGACCAAAATATTAATGGGAATGGCCCAAATGGCATACATGAAGAAGGCTCACCAAGTGAAATGGAAACAGATGAGCCAGATGATGAATCCAGCCAGGATCAAGAACTTCCCTCAGAGAATGAAAACAGTCAGTCTGAAGATTCAGTTGGAGGAGATAATGATTCTGAAAATGGATTATGTACTGAGGATACTTGCAAAGGTCAACTCACGGGACACAAAAAACGATTGTTTACATTCCAGTTCAACAACTTAGGCAATACTGATATCAACTACATCAAAGATGATACCAGGCATATAAGATTTGATGATAGGCAGCTTAGGCTAGATGAAAGATCTTTTCTTGCTTTGGATTGGGATCCTGATTTGAAAAAAAGATATTTTGATGAAAATGCTGCCGAGGACTTTGAAAAACATGAAAGTGTGGAGTATAAACCTCCTAAAAAACCCTTTGTGAAATTAAAAGATTGCATTGAACTTTTTACAACAAAAGAAAAGCTAGGTGCTGAAGATCCCTGGTATTGTCCGAATTGTAAAGAACATCAGCAAGCCACAAAGAAATTGGATTTATGGTCCCTGCCTCCAGTACTTGTAGTACATCTCAAGCGATTTTCTTACAGTCGATACATGAGAGACAAGTTGGATACCTTAGTTGATTTTCCTATCAATGACTTGGATATGTCGGAATTCTTAATTAATCCAAATGCAGGTCCTTGCCGCTATAATCTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGAGGACACTATACTGCTTTTGCAAAAAATAAAGATGATGGAAAATGGTACTATTTTGATGACAGTAGTGTCTCCACTGCATCTGAAGACCAAATTGTGTCCAAAGCAGCATATGTACTCTTCTACCAGAGACAAGACACTTTCAGTGGAACTGGCTTTTTTCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACTGGCATCCCATTAGAAAGTGATGAAGATAGCAATGATAATGACAATGATATAGAAAATGAAAACTGTATGCACACTAACTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSPEFPGRLERPLMAEGGAADLDTQRSDIATLLKTSLRKGD
TWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQS
LKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCK
VEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKETR
LWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPN
VKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTCFMNSAIQCL
SNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYV
TPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQL
KDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVT
FDPFCYLTLPLPMKKERTLEVYLVRMDPLTKPMQYKVVVPKIGNILDLCT
ALSALSGIPADKMIVTDIYNHRFHRIFAMDENLSSIMERDDIYVFEININ
RTEDTEHVIIPVCLREKFRHSSYTHHTGSSLFGQPFLMAVPRNNTEDKLY
NLLLLRMCRYVKISTETEETEGSLHCCKDQNINGNGPNGIHEEGSPSEME
TDEPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEDTCKGQLTGH
KKRLFTFQFNNLGNTDINYIKDDTRHIRFDDRQLRLDERSFLALDWDPDL
KKRYFDENAAEDFEKHESVEYKPPKKPFVKLKDCIELFTTKEKLGAEDPW
YCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPIN
DLDMSEFLINPNAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYF
DDSSVSTASEDQIVSKAAYVLFYQRQDTFSGTGFFPLDRETKGASAATGI
PLESDEDSNDNDNDIENENCMHTN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU19773
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Three silent changes as compared to reference sequence:; T>C 246bp; G>A 1368bp; T>C 2214bp; ; Estimated MW of expressed protein : 111948.94 Daltons