cDNA Clone CDC34

• Synonyms: UBCH3, UBC3, UBE2R1, E2-CDC34, cell division cycle 34
• Plasmid: pET28a CDC34 C191S C223S
• Parent Plasmid: pET28a original
• Expressed: His-CDC34 C191S C223S
• Genbank: P49427 NM_004359 NP_004350 Gene ID 997
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGA
GCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGG
TGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATCTTCGGGCCCCCC
AACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCAT
CGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGC
ACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCG
CCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCC
CACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACG
AGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGG
AAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCG
GAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGG
TGCCCACCACGCTGGCCGAGTACTCCGTGAAGACCAAGGCGCCGGCGCCC
GACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGT
GGAGGAGGAGGCCGACAGCTCCTTCGGGGACGATGAGGATGACTCTGGCA
CGGAGGAGTCCTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSARPLVPSSQKALLLEL
KGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPID
YPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPT
QNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRK
QVLGTKVDAERDGVKVPTTLAEYSVKTKAPAPDEGSDLFYDDYYEDGEVE
EEADSSFGDDEDDSGTEES*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24667
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: native methionine absent. Ala35 of fusion protein = Ala2 of NP_004350