cDNA Clone USP48

• Synonyms: RAP1GA1; USP31
• Plasmid: pcDNA5 FRT/TO BirA R118G-USP48-HA
• Parent Plasmid: pcDNA5 FRT/TO BirA R118G MCS HA
• Expressed: BirA R118G-USP48-HA
• Genbank: NM_032236.5 GI:76257390
• Tag: HA
• Tag: BirA
• Species: Human

Sequence of Insert

GGATCCATGGCCCCGCGGCTGCAGCTGGAGAAGGCGGCCTGGCGCTGGGCGGAGACGGTGCGGCCCGAGGAGGTGTCGCAGGAGCACATCGAGACCGCTTACCGCATCTGGCTGGAGCCCTGCATTCGCGGCGTGTGCAGACGAAACTGCAAAGGAAATCCGAATTGCTTGGTTGGTATTGGTGAGCATATTTGGTTAGGAGAAATAGATGAAAATAGTTTTCATAACATCGATGATCCCAACTGTGAGAGGAGAAAAAAGAACTCATTTGTGGGCCTGACTAACCTTGGAGCCACTTGTTATGTCAACACATTTCTTCAAGTGTGGTTTCTCAACTTGGAGCTTCGGCAGGCACTCTACTTATGTCCAAGCACTTGTAGTGACTACATGCTGGGAGACGGCATCCAAGAAGAAAAAGATTATGAGCCTCAAACAATTTGTGAGCATCTCCAGTACTTGTTTGCCTTGTTGCAAAACAGTAATAGGCGATACATTGATCCATCAGGATTTGTTAAAGCCTTGGGCCTGGACACTGGACAACAGCAGGATGCTCAAGAATTTTCAAAGCTCTTTATGTCTCTATTGGAAGATACTTTGTCTAAACAAAAGAATCCAGATGTGCGCAATATTGTTCAACAGCAGTTCTGTGGAGAATATGCCTATGTAACTGTTTGCAACCAGTGTGGCAGAGAGTCTAAGCTTTTGTCAAAATTTTATGAGCTGGAGTTAAATATCCAAGGCCACAAACAGTTAACAGATTGTATCTCGGAATTTTTGAAGGAAGAAAAATTAGAAGGAGACAATCGCTATTTTTGCGAGAACTGTCAAAGCAAACAGAATGCAACAAGAAAGATTCGACTTCTTAGCCTTCCTTGCACTCTGAACTTGCAGCTAATGCGTTTTGTCTTTGACAGGCAAACTGGACATAAGAAAAAGCTGAATACCTACATTGGCTTCTCAGAAATTTTGGATATGGAGCCTTATGTGGAACATAAAGGTGGGTCCTACGTGTATGAACTCAGCGCAGTCCTCATACACAGAGGAGTGAGTGCTTATTCTGGCCACTACATCGCCCACGTGAAAGATCCACAGTCTGGTGAATGGTATAAGTTTAATGATGAAGACATAGAAAAGATGGAGGGGAAGAAATTACAACTAGGGATTGAGGAAGATCTAGCAGAACCTTCTAAGTCTCAGACACGTAAACCCAAGTGTGGCAAAGGAACTCATTGCTCTCGAAATGCATATATGTTGGTTTATAGACTGCAAACTCAAGAAAAGCCCAACACTACTGTTCAAGTTCCAGCCTTTCTTCAAGAGCTGGTAGATCGGGATAATTCCAAATTTGAGGAGTGGTGTATTGAAATGGCTGAGATGCGTAAGCAAAGTGTGGATAAAGGAAAAGCAAAACACGAAGAGGTTAAGGAGCTGTACCAAAGGTTACCTGCTGGAGCTGAGCCCTATGAGTTTGTCTCTCTGGAATGGCTGCAAAAGTGGTTGGATGAATCAACACCTACCAAACCTATTGATAATCACGCTTGCCTGTGTTCCCATGACAAGCTTCACCCGGATAAAATATCAATTATGAAGAGGATATCTGAATATGCAGCTGACATTTTCTATAGTAGATATGGAGGAGGTCCAAGACTAACTGTGAAAGCCCTGTGTAAGGAATGTGTAGTAGAACGTTGTCGCATATTGCGTCTGAAGAACCAACTAAATGAAGATTATAAAACTGTTAATAATCTGCTGAAAGCAGCAGTAAAGGGCAGCGATGGATTTTGGGTGGGGAAGTCCTCCTTGCGGAGTTGGCGCCAGCTAGCTCTTGAACAGCTGGATGAGCAAGATGGTGATGCAGAACAAAGCAACGGAAAGATGAACGGTAGCACCTTAAATAAAGATGAATCAAAGGAAGAAAGAAAAGAAGAGGAGGAATTAAATTTTAATGAAGATATTCTGTGTCCACATGGTGAGTTATGCATATCTGAAAATGAAAGAAGGCTTGTTTCTAAAGAGGCTTGGAGCAAACTGCAGCAGTACTTTCCAAAGGCTCCTGAGTTTCCAAGTTACAAAGAGTGCTGTTCACAGTGCAAGATTTTAGAAAGAGAAGGGGAAGAAAATGAAGCCTTACATAAGATGATTGCAAACGAGCAAAAGACTTCTCTCCCAAATTTGTTCCAGGATAAAAACAGACCGTGTCTCAGTAACTGGCCAGAGGATACGGATGTCCTCTACATCGTGTCTCAGTTCTTTGTAGAAGAGTGGCGGAAATTTGTTAGAAAGCCTACAAGATGCAGCCCTGTGTCATCAGTTGGGAACAGTGCTCTTTTGTGTCCCCACGGGGGCCTCATGTTTACATTTGCTTCCATGACCAAAGAAGATTCTAAACTTATAGCTCTCATATGGCCCAGTGAGTGGCAAATGATACAAAAGCTCTTTGTTGTGGATCATGTAATTAAAATCACGAGAATTGAAGTGGGAGATGTAAACCCTTCAGAAACACAGTATATTTCTGAGCCCAAACTCTGTCCAGAATGCAGAGAAGGCTTATTGTGTCAGCAGCAGAGGGACCTGCGTGAATACACTCAAGCCACCATCTATGTCCATAAAGTTGTGGATAATAAAAAGGTGATGAAGGATTCGGCTCCGGAACTGAATGTGAGTAGTTCTGAAACAGAGGAGGACAAGGAAGAAGCTAAACCAGATGGAGAAAAAGATCCAGATTTTAATCAAAGCAATGGTGGAACAAAGCGGCAAAAGATATCCCATCAAAATTATATAGCCTATCAAAAGCAAGTTATTCGCCGAAGTATGCGACATAGAAAAGTTCGTGGTGAGAAAGCACTTCTCGTTTCTGCTAATCAGACGTTAAAAGAATTGAAAATTCAGATCATGCATGCATTTTCAGTTGCTCCTTTTGACCAGAATTTGTCAATTGATGGAAAGATTTTAAGTGATGACTGTGCCACCCTAGGCACCCTTGGCGTCATTCCTGAATCTGTCATTTTATTGAAGGCTGATGAACCAATTGCAGATTATGCTGCAATGGATGATGTCATGCAAGTTTGTATGCCAGAAGAAGGGTTTAAAGGTACTGGTCTTCTTGGACATGCGGCCGC

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMAPRLQLEKAAWRWAETVRPEEVSQEH
IETAYRIWLEPCIRGVCRRNCKGNPNCLVGIGEHIWLGEIDENSFHNIDD
PNCERRKKNSFVGLTNLGATCYVNTFLQVWFLNLELRQALYLCPSTCSDY
MLGDGIQEEKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTG
QQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYAYVTVCNQCG
RESKLLSKFYELELNIQGHKQLTDCISEFLKEEKLEGDNRYFCENCQSKQ
NATRKIRLLSLPCTLNLQLMRFVFDRQTGHKKKLNTYIGFSEILDMEPYV
EHKGGSYVYELSAVLIHRGVSAYSGHYIAHVKDPQSGEWYKFNDEDIEKM
EGKKLQLGIEEDLAEPSKSQTRKPKCGKGTHCSRNAYMLVYRLQTQEKPN
TTVQVPAFLQELVDRDNSKFEEWCIEMAEMRKQSVDKGKAKHEEVKELYQ
RLPAGAEPYEFVSLEWLQKWLDESTPTKPIDNHACLCSHDKLHPDKISIM
KRISEYAADIFYSRYGGGPRLTVKALCKECVVERCRILRLKNQLNEDYKT
VNNLLKAAVKGSDGFWVGKSSLRSWRQLALEQLDEQDGDAEQSNGKMNGS
TLNKDESKEERKEEEELNFNEDILCPHGELCISENERRLVSKEAWSKLQQ
YFPKAPEFPSYKECCSQCKILEREGEENEALHKMIANEQKTSLPNLFQDK
NRPCLSNWPEDTDVLYIVSQFFVEEWRKFVRKPTRCSPVSSVGNSALLCP
HGGLMFTFASMTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDV
NPSETQYISEPKLCPECREGLLCQQQRDLREYTQATIYVHKVVDNKKVMK
DSAPELNVSSSETEEDKEEAKPDGEKDPDFNQSNGGTKRQKISHQNYIAY
QKQVIRRSMRHRKVRGEKALLVSANQTLKELKIQIMHAFSVAPFDQNLSI
DGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGF
KGTGLLGHAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49970
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee