cDNA Clone ABIN1

• Synonyms: TNIP1, ABIN-1, NAF1, VAN, nip40-1
• Plasmid: pU6 ABIN1 Nter antisense KI gRNA
• Parent Plasmid: pU6 gRNA BamHI
• Expressed: ABIN1 Nter antisense KI guide RNA
• Genbank: NM_001252385.1
• Species: Human

Sequence of Insert

gTGCCCCCAGGGTCGTAGATC(CGG)

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48506
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Expresses ABIN1 Nter KI antisense guide RNA, 20mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets (extra G added to improve expression from U6 promoter). Targets close to the ATG start codon in exon 2, PAM site situated on antisense strand. Designed to introduce a GFP tag to Abin1 via CRISPR when used in conjunction with the sense guide DU48486 and the donor plasmid DU48524. To be screened for using GFP followed by downstream PCR and Western analysis.