cDNA Clone ZGRF1

• Synonyms: C4orf21
• Plasmid: pFastbac HTb ZGRF1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His ZGRF1
• Genbank: NM_018392.4 GI:254826808 with silent change g5505a
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGAAAGCCAAGAATTTATTGTTCTATATACTCATCAAAAGATGAAGAAGTCAAAAGTGTGGCAAGATGGAATTCTGAAGATCACTCACTTAGGAAACAAAGCAATTTTATATGATGACAAAGGAGCATGTTTGGAGAGTCTGTTTCTTAAATGCCTTGAGGTGAAACCTGGAGATGACTTAGAAAGTGATCGATACTTAATCACAGTTGAAGAGGTTAAAGTTGCTGGAGCCATAGGTATTGTTAAGCAGAATGTCAATAAAGAAGCACCAGAGTTAAATTCAAGGACATTTATATCCTCTGGCCGATCTCTTGGATGTCAGCCCTCTGGCTTAAAAAGGAAGTTTACTGGTTTTCAAGGACCACGTCAGGTTCCAAAGAAAATGGTTATTATGGAAAGTGGTGAATCAGCTGCATCACATGAGGCTAAGAAAACTGGCCCTACTATTTTTTCTCCATTCTGCAGCATGCCTCCTTTGTTTCCTACTGTTGGCAAGAAAGATGTAAATAATATACTGGCAGACCCTGAGAACATTGTGACTTACAAGAACAGGGAGAGAAATGCCATGGATTTTTCTTCGGTTTTTTCTCCATCCTTCCAGATTAACCCAGAAGTGCTGTGTGAAGAAAATTATTTTTGCTCACCTGTCAATTCTGGAAATAAGCTTTCAGACTCTTTACTGACCAATGAGCCTGTGAAAAGAGATAGTTTGGCATCTCACTATTCAGGAGTTTCACAAAACATCAGAAGCAAAGCACAGATATTAGCTCTTCTGAAGTCCGAATCATCTAGTTCATGTGAGGAACTAAATTCTGAGATGACAGAGCATTTTCCTCAAAAACAACCACAAGGAAGTTTAAAAATTGCTACTAAACCAAAGTACCTAATTCAACAGGAAGAGTGTGCTGAGATGAAGAGCACAGAAAATTTATACTACCAGCATCAATCAGAAAATACCATGAGAAATAAAAGCCGGTGGGCCATGTATTTATCCTCACAGAGTTCACCTATACATTCTTCTACTGTAGATGGGAATGATACAGAAAGGAAACCCAAGGCCCAGGAAGATGATGTAAATTCTAATTTGAAAGACCTTTCATTACAAAAAATTATACAGTTCGTTGAAACGTATGCTGAAGAGAGGAAAAAGTATAATGTAGACCAGTCAGTCGGTAATAATGATCCATCCTGGAATCAGGAAGTAAAATTAGAAATTCCTTCATTCAATGAAAGCAGTAGCTTACAGGTTACTTGTAGCAGTGCAGAAAATGATGGTATATTATCAGAATCTGACATTCAAGAAGATAATAAAATACCTTTTAATCAAAATGACAAGGGGTGCATTAAAGGATCAGTTCTCATTAAAGAAAATGCTCAGGAGGTAAATACATGTGGAACACTGGAAAAGGAGTATGAACAATCAGAGTCATCTCTGCCAGAACTGAAACATCTCCAAATTGAATCTAGTAATAATTCTAGGATCTCTGATGACATTACAGACATGATTTCTGAAAGTAAAATGGATAATGAAAGTCTTAATAGTATTCATGAATCTCTGAGTAATGTAACACAACCATTTTTGGAGGTAACTTTTAATCTGAACAATTTTGAGACCAGTGACACTGAGGAGGAATCACAGGAAAGCAACAAAATTTCCCAGGATTCAGAGAGTTGGGTAAAGGACATTTTGGTTAATGATGGCAATTCATGTTTTCAAAAGAGGTCTGAGAATACAAACTGTGAAGAGATTGAGGGTGAACATTTGCCATTCTTAACATCTGTTAGTGACAAACCTACAGTGACATTTCCTGTTAAAGAGACTCTGCCATCACAGTTTTGTGATAAAACTTATGTGGGTTTTGACATGGGAATATGTAAAACTGAAAACACAGGAAAAGAAATAGAGGAGTATAGTGACACATTAAGCAATTTTGAATCTTTCAAGTGGACTGATGCTGTATACGGAGATAATAAAGAAGATGCTAATAAACCTATTCAAGAAGTCAGAATTAACTATGATTTTGCTTTACCCCCGAATAAATCTAAAGGTATAAACATGAATTTACATATTCCTCATATTCAAAACCAGATTGCTGAAAACAGTAATCTATTTTCAGAAGATGCTCAACCTCAGCCTTTTATTTTGGGAAGTGACTTAGACAAAAATGATGAACATGTTTTACCCTCAACTTCTAGTAGTGACAACAGTGTCCAACTATTAAATACCAATCAGAATCACTATGAATGTATTGCACTTGATAAATCAAATACCCACATTTCCAATTCTTTGTTTTACCCACTGGGAAAAAAGCATCTTATTTCCAAAGACACAGAAGCACATATATCTGAACCTGAAGATTTGGGAAAGATTAGAAGTCCACCCCCTGACCATGTTGAGGTTGAAACTGCCAGAGAAGGCAAACAATACTGGAATCCTAGAAATTCTTCAGAACTTTCTGGATTAGTAAATACCATTTCTATTTTAAAGTCGCTATGTGAACACAGTACTGCTTTAGACAGCTTGGAAATATTGAAAAAGAAAAATACTGTCTTTCAGCAAGGAACTCAACAGACGTATGAGCCAGATAGCCCTCCTGAAGTGAGGAAACCATTTATTACAGTAGTTTCACCAAAGTCTCCTCATCTGCACAAGGATTCTCAACAGATACTAAAAGAAGATGAAGTTGAACTAAGTGAACCACTTCAGTCTGTGCAGTTCTCTTCCTCGGGAAGTAAAGAAGAGACTGCTTTTCAAGCTGTTATTCCTAAACAAATAGAGAGAAAAACCTGTGACCCTAAGCCTGTTGAGTTTCAAGGACATCAAGTAAAAGGATCAGCTACTAGTGGTGTAATGGTCAGAGGACACAGCTCACAGCTAGGATGCAGTCAGTTTCCAGATAGCACTGAGTATGAAAACTTTATGACAGAGACACCAGAGTTACCTAGCACGTGTATGCAGATTGACTTCTTGCAGGTGACATCACCAGAAGAAAACATCTCTACATTGAGCCCTGTTTCTACCTTTTCTTTGAACTCAAGAGATGAAGACTTCATGGTAGAATTCTCTGAGACGTCCCTGAAAGCAAGAACTTTACCTGATGATCTTCATTTTCTCAACTTGGAGGGGATGAAAAAATCCCGTTCTCTGGAGAATGAGAACCTTCAAAGGCTTTCATTATTAAGTAGAACCCAGGTTCCACTTATTACTTTGCCACGTACTGATGGGCCACCTGACTTAGACTCTCATTCGTATATGATCAACTCTAACACATACGAGTCTTCTGGCTCCCCCATGCTCAATTTGTGTGAAAAGTCAGCAGTTCTTTCGTTTAGCATTGAGCCTGAGGACCAAAATGAAACCTTTTTCTCTGAAGAATCTAGGGAAGTGAATCCAGGGGATGTTTCACTTAATAATATATCTACTCAGAGCAAGTGGCTGAAATATCAAAACACATCCCAATGCAACGTGGCTACTCCAAACAGAGTTGATAAGAGAATAACTGATGGCTTCTTTGCTGAGGCTGTTTCTGGGATGCATTTTAGAGACACAAGTGAAAGACAGAGTGATGCTGTCAATGAAAGCTCTTTAGACTCTGTGCATTTGCAAATGATAAAAGGCATGCTCTATCAACAGCGGCAGGATTTTAGCAGTCAAGATTCGGTTTCCAGAAAGAAAGTACTTTCTCTGAATTTAAAGCAGACTTCTAAGACAGAGGAAATTAAAAATGTATTAGGAGGGTCTACCTGCTACAACTACAGTGTAAAGGATTTACAGGAGATAAGTGGCTCTGAGCTGTGCTTTCCAAGTGGGCAGAAAATAAAATCTGCTTATCTTCCCCAAAGGCAAATTCACATACCAGCTGTTTTTCAGTCTCCTGCTCATTATAAGCAGACTTTCACATCTTGCCTCATAGAACATCTAAATATATTGCTGTTTGGGTTAGCACAAAACCTGCAGAAAGCTCTTTCAAAAGTTGACATATCATTTTATACATCATTGAAGGGAGAGAAACTGAAAAACGCAGAAAATAATGTACCATCCTGCCATCATAGTCAACCTGCAAAACTTGTCATGGTTAAAAAGGAAGGTCCAAATAAGGGTCGTCTCTTTTATACATGTGATGGACCCAAAGCTGATCGATGTAAATTCTTTAAATGGCTTGAGGACGTGACTCCAGGATATTCAACACAGGAAGGAGCTCGACCTGGCATGGTTTTAAGTGATATTAAGAGTATTGGCTTATATTTAAGAAGTCAAAAGATACCACTTTATGAGGAATGCCAGCTTTTGGTGAGAAAAGGATTTGATTTTCAGAGAAAACAGTATGGCAAACTAAAGAAGTTTACTACTGTAAATCCTGAGTTTTATAATGAACCAAAAACCAAACTTTATCTTAAGCTAAGTCGGAAGGAAAGATCTTCAGCTTATAGCAAAAATGATCTTTGGGTGGTTTCAAAAACCCTAGACTTTGAGCTGGATACTTTTATCGCATGTAGTGCTTTCTTTGGACCATCATCTATCAATGAGATAGAAATACTGCCTTTGAAAGGCTATTTCCCTTCTAATTGGCCCACTAACATGGTTGTCCATGCGTTATTGGTTTGTAATGCTAGCACAGAACTGACTACTTTGAAAAACATTCAGGACTACTTTAATCCAGCTACTCTACCTCTAACACAGTACCTGTTAACAACGTCTTCGCCAACTATAGTTAGTAACAAAAGAGTCAGTAAGAGAAAATTTATCCCACCAGCCTTCACAAATGTCAGTACAAAATTTGAACTACTCAGCCTAGGAGCAACATTGAAGTTAGCTAGTGAGTTGATTCAGGTACACAAGTTAAACAAGGATCAAGCTACAGCTCTAATTCAAATAGCTCAAATGATGGCATCACATGAAAGCATTGAAGAAGTGAAGGAACTGCAAACTCATACCTTCCCTATCACAATCATACATGGTGTGTTTGGAGCAGGAAAGAGTTACTTGCTGGCAGTGGTGATTTTGTTCTTTGTACAGCTGTTTGAAAAGAGTGAAGCTCCCACCATTGGAAATGCAAGGCCGTGGAAACTTCTGATTTCTTCTTCTACTAATGTGGCTGTTGACAGAGTACTTCTTGGGCTTCTCAGTCTTGGATTTGAAAACTTTATCAGAGTTGGGAGTGTTAGGAAGATTGCCAAACCAATTTTACCTTATAGCTTGCATGCTGGCTCAGAAAATGAAAGTGAACAGTTAAAAGAACTACATGCACTAATGAAAGAAGACCTGACTCCTACGGAAAGAGTCTATGTGAGAAAAAGCATTGAGCAGCATAAACTGGGGACCAATAGAACCCTGCTGAAGCAGGTTCGAGTAGTTGGAGTTACCTGTGCAGCCTGCCCATTCCCATGCATGAATGATCTTAAATTTCCTGTAGTTGTGCTGGATGAGTGTAGTCAGATAACTGAACCGGCCTCTCTCCTTCCCATTGCAAGGTTTGAGTGTGAAAAGCTGATTCTTGTTGGAGATCCCAAACAGCTACCTCCTACTATTCAGGGTTCTGATGCAGCTCATGAAAATGGATTGGAACAAACTCTTTTTGATCGACTTTGCTTAATGGGTCACAAGCCAATTCTATTGAGAACTCAATACCGTTGTCATCCTGCAATCAGTGCTATTGCTAATGATCTGTTTTACAAAGGAGCCCTCATGAATGGTGTAACAGAAATAGAGCGGAGCCCTTTATTGGAATGGCTACCAACCCTGTGTTTTTATAATGTTAAAGGACTAGAACAGATAGAAAGAGATAACAGCTTTCATAATGTGGCAGAAGCTACGTTTACACTCAAGCTGATTCAATCACTGATTGCAAGTGGAATAGCAGGCTCTATGATTGGTGTGATAACATTATACAAATCCCAGATGTACAAGCTTTGTCATTTACTCAGTGCTGTGGACTTTCACCATCCTGATATTAAAACTGTGCAGGTGTCCACAGTAGATGCTTTTCAGGGAGCTGAAAAGGAGATCATTATTCTGTCCTGTGTAAGGACAAGACAAGTAGGATTCATTGATTCAGAAAAAAGAATGAATGTTGCATTGACTAGAGGAAAGAGGCATTTGTTGATTGTGGGAAATTTAGCCTGTTTGAGGAAAAATCAACTTTGGGGACGAGTGATCCAACACTGCGAAGGAAGGGAAGATGGATTGCAACATGCAAACCAGTATGAACCACAGCTGAACCATCTCCTTAAAGATTATTTTGAAAAACAAGTGGAAGAAAAACAGAAGAAAAAGAGTGAAAAAGAGAAATCTAAAGATAAATCTCATTCATAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMESQEFIVLYTHQKMKKSKVWQ
DGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITVEE
VKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGP
RQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNI
LADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNK
LSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNS
EMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMR
NKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKII
QFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSA
ENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEY
EQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSN
VTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCF
QKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFD
MGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRIN
YDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDK
NDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKH
LISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGL
VNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPF
ITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQAVI
PKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYE
NFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEF
SETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLP
RTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETF
FSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFF
AEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSV
SRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQ
KIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKA
LSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFY
TCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKI
PLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKE
RSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPS
NWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIV
SNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATA
LIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFV
QLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSV
RKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHK
LGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLP
IARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPIL
LRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKG
LEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLC
HLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKR
MNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQ
LNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU49911
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Recommend propagation at 30 degrees.