cDNA Clone ZGRF1

• Synonyms: C4orf21
• Plasmid: pFastbacDual GST 6P1 ZGRF1
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST 3C ZGRF1
• Genbank: NM_018392.4 GI:254826808 with silent change g5505a
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAAGCCAAGAATTTATTGTTCTATATACTCATCAAAAGATGAAGAAGTCAAAAGTGTGGCAAGATGGAATTCTGAAGATCACTCACTTAGGAAACAAAGCAATTTTATATGATGACAAAGGAGCATGTTTGGAGAGTCTGTTTCTTAAATGCCTTGAGGTGAAACCTGGAGATGACTTAGAAAGTGATCGATACTTAATCACAGTTGAAGAGGTTAAAGTTGCTGGAGCCATAGGTATTGTTAAGCAGAATGTCAATAAAGAAGCACCAGAGTTAAATTCAAGGACATTTATATCCTCTGGCCGATCTCTTGGATGTCAGCCCTCTGGCTTAAAAAGGAAGTTTACTGGTTTTCAAGGACCACGTCAGGTTCCAAAGAAAATGGTTATTATGGAAAGTGGTGAATCAGCTGCATCACATGAGGCTAAGAAAACTGGCCCTACTATTTTTTCTCCATTCTGCAGCATGCCTCCTTTGTTTCCTACTGTTGGCAAGAAAGATGTAAATAATATACTGGCAGACCCTGAGAACATTGTGACTTACAAGAACAGGGAGAGAAATGCCATGGATTTTTCTTCGGTTTTTTCTCCATCCTTCCAGATTAACCCAGAAGTGCTGTGTGAAGAAAATTATTTTTGCTCACCTGTCAATTCTGGAAATAAGCTTTCAGACTCTTTACTGACCAATGAGCCTGTGAAAAGAGATAGTTTGGCATCTCACTATTCAGGAGTTTCACAAAACATCAGAAGCAAAGCACAGATATTAGCTCTTCTGAAGTCCGAATCATCTAGTTCATGTGAGGAACTAAATTCTGAGATGACAGAGCATTTTCCTCAAAAACAACCACAAGGAAGTTTAAAAATTGCTACTAAACCAAAGTACCTAATTCAACAGGAAGAGTGTGCTGAGATGAAGAGCACAGAAAATTTATACTACCAGCATCAATCAGAAAATACCATGAGAAATAAAAGCCGGTGGGCCATGTATTTATCCTCACAGAGTTCACCTATACATTCTTCTACTGTAGATGGGAATGATACAGAAAGGAAACCCAAGGCCCAGGAAGATGATGTAAATTCTAATTTGAAAGACCTTTCATTACAAAAAATTATACAGTTCGTTGAAACGTATGCTGAAGAGAGGAAAAAGTATAATGTAGACCAGTCAGTCGGTAATAATGATCCATCCTGGAATCAGGAAGTAAAATTAGAAATTCCTTCATTCAATGAAAGCAGTAGCTTACAGGTTACTTGTAGCAGTGCAGAAAATGATGGTATATTATCAGAATCTGACATTCAAGAAGATAATAAAATACCTTTTAATCAAAATGACAAGGGGTGCATTAAAGGATCAGTTCTCATTAAAGAAAATGCTCAGGAGGTAAATACATGTGGAACACTGGAAAAGGAGTATGAACAATCAGAGTCATCTCTGCCAGAACTGAAACATCTCCAAATTGAATCTAGTAATAATTCTAGGATCTCTGATGACATTACAGACATGATTTCTGAAAGTAAAATGGATAATGAAAGTCTTAATAGTATTCATGAATCTCTGAGTAATGTAACACAACCATTTTTGGAGGTAACTTTTAATCTGAACAATTTTGAGACCAGTGACACTGAGGAGGAATCACAGGAAAGCAACAAAATTTCCCAGGATTCAGAGAGTTGGGTAAAGGACATTTTGGTTAATGATGGCAATTCATGTTTTCAAAAGAGGTCTGAGAATACAAACTGTGAAGAGATTGAGGGTGAACATTTGCCATTCTTAACATCTGTTAGTGACAAACCTACAGTGACATTTCCTGTTAAAGAGACTCTGCCATCACAGTTTTGTGATAAAACTTATGTGGGTTTTGACATGGGAATATGTAAAACTGAAAACACAGGAAAAGAAATAGAGGAGTATAGTGACACATTAAGCAATTTTGAATCTTTCAAGTGGACTGATGCTGTATACGGAGATAATAAAGAAGATGCTAATAAACCTATTCAAGAAGTCAGAATTAACTATGATTTTGCTTTACCCCCGAATAAATCTAAAGGTATAAACATGAATTTACATATTCCTCATATTCAAAACCAGATTGCTGAAAACAGTAATCTATTTTCAGAAGATGCTCAACCTCAGCCTTTTATTTTGGGAAGTGACTTAGACAAAAATGATGAACATGTTTTACCCTCAACTTCTAGTAGTGACAACAGTGTCCAACTATTAAATACCAATCAGAATCACTATGAATGTATTGCACTTGATAAATCAAATACCCACATTTCCAATTCTTTGTTTTACCCACTGGGAAAAAAGCATCTTATTTCCAAAGACACAGAAGCACATATATCTGAACCTGAAGATTTGGGAAAGATTAGAAGTCCACCCCCTGACCATGTTGAGGTTGAAACTGCCAGAGAAGGCAAACAATACTGGAATCCTAGAAATTCTTCAGAACTTTCTGGATTAGTAAATACCATTTCTATTTTAAAGTCGCTATGTGAACACAGTACTGCTTTAGACAGCTTGGAAATATTGAAAAAGAAAAATACTGTCTTTCAGCAAGGAACTCAACAGACGTATGAGCCAGATAGCCCTCCTGAAGTGAGGAAACCATTTATTACAGTAGTTTCACCAAAGTCTCCTCATCTGCACAAGGATTCTCAACAGATACTAAAAGAAGATGAAGTTGAACTAAGTGAACCACTTCAGTCTGTGCAGTTCTCTTCCTCGGGAAGTAAAGAAGAGACTGCTTTTCAAGCTGTTATTCCTAAACAAATAGAGAGAAAAACCTGTGACCCTAAGCCTGTTGAGTTTCAAGGACATCAAGTAAAAGGATCAGCTACTAGTGGTGTAATGGTCAGAGGACACAGCTCACAGCTAGGATGCAGTCAGTTTCCAGATAGCACTGAGTATGAAAACTTTATGACAGAGACACCAGAGTTACCTAGCACGTGTATGCAGATTGACTTCTTGCAGGTGACATCACCAGAAGAAAACATCTCTACATTGAGCCCTGTTTCTACCTTTTCTTTGAACTCAAGAGATGAAGACTTCATGGTAGAATTCTCTGAGACGTCCCTGAAAGCAAGAACTTTACCTGATGATCTTCATTTTCTCAACTTGGAGGGGATGAAAAAATCCCGTTCTCTGGAGAATGAGAACCTTCAAAGGCTTTCATTATTAAGTAGAACCCAGGTTCCACTTATTACTTTGCCACGTACTGATGGGCCACCTGACTTAGACTCTCATTCGTATATGATCAACTCTAACACATACGAGTCTTCTGGCTCCCCCATGCTCAATTTGTGTGAAAAGTCAGCAGTTCTTTCGTTTAGCATTGAGCCTGAGGACCAAAATGAAACCTTTTTCTCTGAAGAATCTAGGGAAGTGAATCCAGGGGATGTTTCACTTAATAATATATCTACTCAGAGCAAGTGGCTGAAATATCAAAACACATCCCAATGCAACGTGGCTACTCCAAACAGAGTTGATAAGAGAATAACTGATGGCTTCTTTGCTGAGGCTGTTTCTGGGATGCATTTTAGAGACACAAGTGAAAGACAGAGTGATGCTGTCAATGAAAGCTCTTTAGACTCTGTGCATTTGCAAATGATAAAAGGCATGCTCTATCAACAGCGGCAGGATTTTAGCAGTCAAGATTCGGTTTCCAGAAAGAAAGTACTTTCTCTGAATTTAAAGCAGACTTCTAAGACAGAGGAAATTAAAAATGTATTAGGAGGGTCTACCTGCTACAACTACAGTGTAAAGGATTTACAGGAGATAAGTGGCTCTGAGCTGTGCTTTCCAAGTGGGCAGAAAATAAAATCTGCTTATCTTCCCCAAAGGCAAATTCACATACCAGCTGTTTTTCAGTCTCCTGCTCATTATAAGCAGACTTTCACATCTTGCCTCATAGAACATCTAAATATATTGCTGTTTGGGTTAGCACAAAACCTGCAGAAAGCTCTTTCAAAAGTTGACATATCATTTTATACATCATTGAAGGGAGAGAAACTGAAAAACGCAGAAAATAATGTACCATCCTGCCATCATAGTCAACCTGCAAAACTTGTCATGGTTAAAAAGGAAGGTCCAAATAAGGGTCGTCTCTTTTATACATGTGATGGACCCAAAGCTGATCGATGTAAATTCTTTAAATGGCTTGAGGACGTGACTCCAGGATATTCAACACAGGAAGGAGCTCGACCTGGCATGGTTTTAAGTGATATTAAGAGTATTGGCTTATATTTAAGAAGTCAAAAGATACCACTTTATGAGGAATGCCAGCTTTTGGTGAGAAAAGGATTTGATTTTCAGAGAAAACAGTATGGCAAACTAAAGAAGTTTACTACTGTAAATCCTGAGTTTTATAATGAACCAAAAACCAAACTTTATCTTAAGCTAAGTCGGAAGGAAAGATCTTCAGCTTATAGCAAAAATGATCTTTGGGTGGTTTCAAAAACCCTAGACTTTGAGCTGGATACTTTTATCGCATGTAGTGCTTTCTTTGGACCATCATCTATCAATGAGATAGAAATACTGCCTTTGAAAGGCTATTTCCCTTCTAATTGGCCCACTAACATGGTTGTCCATGCGTTATTGGTTTGTAATGCTAGCACAGAACTGACTACTTTGAAAAACATTCAGGACTACTTTAATCCAGCTACTCTACCTCTAACACAGTACCTGTTAACAACGTCTTCGCCAACTATAGTTAGTAACAAAAGAGTCAGTAAGAGAAAATTTATCCCACCAGCCTTCACAAATGTCAGTACAAAATTTGAACTACTCAGCCTAGGAGCAACATTGAAGTTAGCTAGTGAGTTGATTCAGGTACACAAGTTAAACAAGGATCAAGCTACAGCTCTAATTCAAATAGCTCAAATGATGGCATCACATGAAAGCATTGAAGAAGTGAAGGAACTGCAAACTCATACCTTCCCTATCACAATCATACATGGTGTGTTTGGAGCAGGAAAGAGTTACTTGCTGGCAGTGGTGATTTTGTTCTTTGTACAGCTGTTTGAAAAGAGTGAAGCTCCCACCATTGGAAATGCAAGGCCGTGGAAACTTCTGATTTCTTCTTCTACTAATGTGGCTGTTGACAGAGTACTTCTTGGGCTTCTCAGTCTTGGATTTGAAAACTTTATCAGAGTTGGGAGTGTTAGGAAGATTGCCAAACCAATTTTACCTTATAGCTTGCATGCTGGCTCAGAAAATGAAAGTGAACAGTTAAAAGAACTACATGCACTAATGAAAGAAGACCTGACTCCTACGGAAAGAGTCTATGTGAGAAAAAGCATTGAGCAGCATAAACTGGGGACCAATAGAACCCTGCTGAAGCAGGTTCGAGTAGTTGGAGTTACCTGTGCAGCCTGCCCATTCCCATGCATGAATGATCTTAAATTTCCTGTAGTTGTGCTGGATGAGTGTAGTCAGATAACTGAACCGGCCTCTCTCCTTCCCATTGCAAGGTTTGAGTGTGAAAAGCTGATTCTTGTTGGAGATCCCAAACAGCTACCTCCTACTATTCAGGGTTCTGATGCAGCTCATGAAAATGGATTGGAACAAACTCTTTTTGATCGACTTTGCTTAATGGGTCACAAGCCAATTCTATTGAGAACTCAATACCGTTGTCATCCTGCAATCAGTGCTATTGCTAATGATCTGTTTTACAAAGGAGCCCTCATGAATGGTGTAACAGAAATAGAGCGGAGCCCTTTATTGGAATGGCTACCAACCCTGTGTTTTTATAATGTTAAAGGACTAGAACAGATAGAAAGAGATAACAGCTTTCATAATGTGGCAGAAGCTACGTTTACACTCAAGCTGATTCAATCACTGATTGCAAGTGGAATAGCAGGCTCTATGATTGGTGTGATAACATTATACAAATCCCAGATGTACAAGCTTTGTCATTTACTCAGTGCTGTGGACTTTCACCATCCTGATATTAAAACTGTGCAGGTGTCCACAGTAGATGCTTTTCAGGGAGCTGAAAAGGAGATCATTATTCTGTCCTGTGTAAGGACAAGACAAGTAGGATTCATTGATTCAGAAAAAAGAATGAATGTTGCATTGACTAGAGGAAAGAGGCATTTGTTGATTGTGGGAAATTTAGCCTGTTTGAGGAAAAATCAACTTTGGGGACGAGTGATCCAACACTGCGAAGGAAGGGAAGATGGATTGCAACATGCAAACCAGTATGAACCACAGCTGAACCATCTCCTTAAAGATTATTTTGAAAAACAAGTGGAAGAAAAACAGAAGAAAAAGAGTGAAAAAGAGAAATCTAAAGATAAATCTCATTCATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESQEFIVLYTHQKMKKSK
VWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLIT
VEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGF
QGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDV
NNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNS
GNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEE
LNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSEN
TMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQ
KIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTC
SSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLE
KEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHES
LSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGN
SCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYV
GFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEV
RINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSD
LDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLG
KKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSEL
SGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVR
KPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQ
AVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDST
EYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFM
VEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLI
TLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQN
ETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITD
GFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQ
DSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFP
SGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNL
QKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGR
LFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRS
QKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLS
RKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGY
FPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSP
TIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQ
ATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVIL
FFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRV
GSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIE
QHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPAS
LLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHK
PILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYN
VKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMY
KLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDS
EKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQY
EPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU49910
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Recommend propagation at 30 degrees.