cDNA Clone USP43

• Plasmid: pcDNA5 FRT/TO BirA R118G-USP43 C110A-HA
• Parent Plasmid: pcDNA5 FRT/TO BirA R118G MCS HA
• Expressed: BirA R118G-USP43 C110A-HA
• Genbank: NM_153210.4
• Tag: HA
• Tag: BirA
• Species: Human

Sequence of Insert

GAATTCATGGACCTGGGCCCCGGGGACGCGGCAGGAGGGGGACCGCTCGCGCCCCGGCCCCGCCGCCGCCGCTCCCTGCGCCGCCTGTTCAGCCGCTTCCTGCTGGCGCTGGGCAGCCGCTCACGCCCCGGGGACTCACCGCCCCGGCCCCAGCCGGGACACTGTGATGGCGACGGTGAGGGGGGCTTCGCCTGCGCCCCGGGCCCAGTTCCAGCGGCCCCCGGGAGCCCCGGGGAGGAACGCCCGCCCGGACCCCAGCCCCAGCTCCAGCTCCCCGCCGGCGATGGGGCTCGGCCTCCGGGCGCTCAGGGCTTGAAGAACCACGGCAACACCGCCTTCATGAACGCGGTGGTGCAGTGTCTCAGCAACACCGACCTGCTGGCCGAGTTCCTGGCGCTGGGGCGCTACCGGGCGGCTCCGGGCCGCGCCGAGGTCACCGAGCAGCTGGCGGCGCTGGTGCGCGCGCTCTGGACTCGCGAATACACGCCCCAACTTTCCGCGGAGTTCAAGAATGCAGTTTCCAAGTACGGCTCTCAGTTCCAAGGCAATTCCCAGCACGACGCCCTGGAGTTCCTGCTCTGGTTGCTGGATCGTGTACATGAGGACCTGGAGGGTTCATCCCGAGGGCCGGTGTCGGAGAAGCTTCCGCCTGAAGCCACTAAAACCTCTGAGAACTGCCTGTCACCATCAGCTCAGCTTCCTCTAGGTCAAAGCTTTGTGCAAAGCCACTTTCAAGCACAATATAGATCTTCCTTGACTTGTCCCCACTGCCTGAAACAGAGCAACACCTTTGATCCTTTCCTGTGTGTGTCCCTACCTATCCCCTTGCGCCAGACGAGGTTCTTGAGTGTCACCTTGGTCTTCCCCTCTAAGAGCCAGCGGTTCCTGCGGGTTGGCCTGGCCGTGCCGATCCTCAGCACAGTGGCAGCCCTGAGGAAGATGGTTGCAGAGGAAGGAGGCGTCCCTGCAGATGAGGTGATCTTGGTTGAACTGTATCCCAGTGGATTCCAGCGGTCTTTCTTTGATGAAGAGGACCTGAATACCATCGCAGAGGGAGATAATGTGTATGCCTTTCAAGTTCCTCCCTCACCCAGCCAGGGGACTCTCTCAGCTCATCCACTGGGTCTGTCGGCCTCCCCACGCCTGGCAGCCCGTGAGGGCCAGCGATTCTCCCTCTCTCTCCACAGTGAGAGCAAGGTGCTAATCCTCTTCTGTAACTTGGTGGGGTCAGGGCAGCAGGCTAGCAGGTTTGGGCCACCCTTCCTGATAAGGGAAGACAGAGCTGTTTCCTGGGCCCAGCTCCAGCAGTCTATCCTCAGCAAGGTCCGCCATCTTATGAAGAGTGAGGCCCCTGTACAGAACCTGGGGTCTCTGTTCTCCATCCGTGTTGTGGGACTCTCTGTGGCCTGCAGCTATTTGTCTCCGAAGGACAGTCGGCCCCTCTGTCACTGGGCAGTTGACAGGGTTTTGCATCTCAGGAGGCCAGGAGGCCCTCCACATGTCAAGCTGGCGGTGGAGTGGGATAGCTCTGTCAAGGAGCGCCTGTTCGGGAGCCTCCAGGAGGAGCGAGCGCAGGATGCCGACAGTGTGTGGCAGCAGCAGCAGGCGCATCAGCAGCACAGCTGTACCTTGGATGAATGTTTTCAGTTCTACACCAAGGAGGAGCAGCTGGCCCAGGATGACGCCTGGAAGTGTCCTCACTGCCAAGTCCTGCAGCAGGGGATGGTGAAGCTGAGTTTGTGGACGCTGCCTGACATCCTCATCATCCACCTCAAAAGGTTCTGCCAGGTGGGCGAGAGAAGAAACAAGCTCTCCACGCTGGTGAAGTTTCCGCTCTCTGGACTCAACATGGCTCCCCATGTGGCCCAGAGAAGCACCAGCCCTGAGGCAGGACTGGGCCCCTGGCCTTCCTGGAAGCAGCCGGACTGCCTGCCCACCAGTTACCCGCTGGACTTCCTGTACGACCTGTATGCCGTCTGCAACCACCATGGCAACCTGCAAGGTGGGCATTACACAGCCTACTGCCGGAACTCTCTGGATGGCCAGTGGTACAGTTATGATGACAGCACGGTGGAACCGCTTCGAGAAGATGAGGTCAACACCAGAGGGGCTTATATCCTGTTCTATCAGAAGCGGAACAGCATCCCTCCCTGGTCAGCCAGCAGCTCCATGAGAGGCTCTACCAGCTCCTCCCTGTCTGATCACTGGCTCTTACGGCTCGGGAGCCACGCTGGCAGCACAAGGGGAAGCCTGCTGTCCTGGAGCTCTGCCCCCTGCCCCTCCCTGCCCCAGGTTCCTGACTCTCCCATCTTCACCAACAGCCTCTGCAATCAGGAAAAGGGAGGGTTGGAGCCCAGGCGTTTGGTACGGGGCGTGAAAGGCAGAAGCATTAGCATGAAGGCACCCACCACTTCCCGAGCCAAGCAGGGACCATTCAAGACCATGCCTCTGCGGTGGTCCTTTGGATCCAAGGAGAAACCACCAGGTGCCTCCGTCGAGTTGGTGGAGTACTTGGAATCCAGACGAAGACCTCGGTCCACGAGCCAGTCCATTGTGTCGCTGTTGACGGGCACTGCGGGTGAGGATGAGAAGTCAGCATCGCCGAGGTCCAACGTCGCCCTTCCTGCTAACAGCGAAGATGGTGGGCGGGCCATTGAAAGAGGTCCAGCCGGGGTGCCCTGTCCCTCGGCTCAACCCAACCACTGTCTGGCCCCTGGAAACTCAGATGGTCCAAACACAGCAAGGAAACTCAAGGAAAATGCAGGGCAGGACATCAAGCTTCCCAGAAAGTTTGACCTGCCTCTCACTGTGATGCCTTCAGTGGAGCATGAGAAACCAGCTCGACCGGAGGGCCAGAAGGCCATGAACTGGAAGGAGAGCTTCCAGATGGGAAGCAAAAGCAGCCCACCCTCCCCCTATATGGGATTCTCTGGAAACAGCAAAGACAGTCGCCGAGGCACCTCTGAGCTAGACAGACCCCTGCAGGGGACACTCACCCTTCTGAGGTCCGTGTTTCGGAAGAAGGAGAACAGGAGGAATGAGAGGGCAGAGGTCTCTCCACAGGTGCCCCCCGTCTCCCTGGTGAGTGGCGGGCTGAGCCCTGCCATGGACGGGCAGGCTCCAGGCTCACCTCCTGCCCTCAGGATCCCAGAGGGCCTGGCCAGGGGCCTGGGCAGCCGGCTCGAGAGGGATGTCTGGTCAGCCCCCAGCTCTCTCCGCCTCCCTCGTAAAGCCAGCAGGGCCCCGAGAGGCAGTGCACTGGGCATGTCACAAAGGACTGTTCCAGGGGAGCAGGCTTCTTATGGCACCTTTCAGAGAGTCAAATATCACACTCTTTCTTTAGGTCGAAAGAAAACCTTACCGGAGTCCAGCTTTGCGGCCGC

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSPGEFMDLGPGDAAGGGPLAPRPRRRRS
LRRLFSRFLLALGSRSRPGDSPPRPQPGHCDGDGEGGFACAPGPVPAAPG
SPGEERPPGPQPQLQLPAGDGARPPGAQGLKNHGNTAFMNAVVQCLSNTD
LLAEFLALGRYRAAPGRAEVTEQLAALVRALWTREYTPQLSAEFKNAVSK
YGSQFQGNSQHDALEFLLWLLDRVHEDLEGSSRGPVSEKLPPEATKTSEN
CLSPSAQLPLGQSFVQSHFQAQYRSSLTCPHCLKQSNTFDPFLCVSLPIP
LRQTRFLSVTLVFPSKSQRFLRVGLAVPILSTVAALRKMVAEEGGVPADE
VILVELYPSGFQRSFFDEEDLNTIAEGDNVYAFQVPPSPSQGTLSAHPLG
LSASPRLAAREGQRFSLSLHSESKVLILFCNLVGSGQQASRFGPPFLIRE
DRAVSWAQLQQSILSKVRHLMKSEAPVQNLGSLFSIRVVGLSVACSYLSP
KDSRPLCHWAVDRVLHLRRPGGPPHVKLAVEWDSSVKERLFGSLQEERAQ
DADSVWQQQQAHQQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGM
VKLSLWTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRS
TSPEAGLGPWPSWKQPDCLPTSYPLDFLYDLYAVCNHHGNLQGGHYTAYC
RNSLDGQWYSYDDSTVEPLREDEVNTRGAYILFYQKRNSIPPWSASSSMR
GSTSSSLSDHWLLRLGSHAGSTRGSLLSWSSAPCPSLPQVPDSPIFTNSL
CNQEKGGLEPRRLVRGVKGRSISMKAPTTSRAKQGPFKTMPLRWSFGSKE
KPPGASVELVEYLESRRRPRSTSQSIVSLLTGTAGEDEKSASPRSNVALP
ANSEDGGRAIERGPAGVPCPSAQPNHCLAPGNSDGPNTARKLKENAGQDI
KLPRKFDLPLTVMPSVEHEKPARPEGQKAMNWKESFQMGSKSSPPSPYMG
FSGNSKDSRRGTSELDRPLQGTLTLLRSVFRKKENRRNERAEVSPQVPPV
SLVSGGLSPAMDGQAPGSPPALRIPEGLARGLGSRLERDVWSAPSSLRLP
RKASRAPRGSALGMSQRTVPGEQASYGTFQRVKYHTLSLGRKKTLPESSF
AAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50016
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee