cDNA Clone UBE2Z

• Synonyms: ubiquitin-conjugating enzyme E2Z (UBE2Z), Use1
• Plasmid: pET15b 6HIS TEV UBE2Z delta Met1-Ile108
• Parent Plasmid: pET15b original
• Expressed: 6His-UBE2Z delta Met1-Ile108
• Genbank: NM_023079.3 GI:150417998 Uniprot Q9H832
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTCCATTTATAAGGAGCCTCCTCCAGGAATGTTCGTTGTACCTGATACTGTTGACATGACTAAGATTCATGCATTGATCACAGGCCCATTTGACACTCCTTATGAAGGGGGTTTCTTCCTGTTCGTGTTTCGGTGTCCGCCCGACTATCCCATCCACCCACCTCGGGTCAAACTGATGACAACGGGCAATAACACAGTGAGGTTTAACCCCAACTTCTACCGCAATGGGAAAGTCTGCTTGAGTATTCTAGGTACATGGACTGGACCTGCCTGGAGCCCAGCCCAGAGCATCTCCTCAGTGCTCATCTCTATCCAGTCCCTGATGACTGAGAACCCCTATCACAATGAGCCCGGCTTTGAACAGGAGAGACATCCAGGAGACAGCAAAAACTATAATGAATGTATCCGGCACGAGACCATCAGAGTTGCAGTCTGTGACATGATGGAAGGAAAGTGTCCCTGTCCTGAACCCCTACGAGGGGTGATGGAGAAGTCCTTTCTGGAGTATTACGACTTCTATGAGGTGGCCTGCAAAGATCGCCTGCACCTTCAAGGCCAAACTATGCAGGACCCTTTTGGAGAGAAGCGGGGCCACTTTGACTACCAGTCCCTCTTGATGCGCCTGGGACTGATACGTCAGAAAGTGCTGGAGAGGCTCCATAATGAGAATGCAGAAATGGACTCTGATAGCAGTTCATCTGGGACAGAGACAGACCTTCATGGGAGCCTGAGGGTTTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGENLYFQGGSMSIYKEPPPGMFVVPDTVDMTKIHALIT
GPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRFNPNFYRNGK
VCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGD
SKNYNECIRHETIRVAVCDMMEGKCPCPEPLRGVMEKSFLEYYDFYEVAC
KDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLERLHNENAEMD
SDSSSSGTETDLHGSLRV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24519
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_023079.3 CDS: c846t (silent). Identical to 24518 except C3 cleavage changed to TEV