cDNA Clone WNK1

• Synonyms: HSAN2, HSN2, KDP, PRKWNK1, PSK, p65
• Plasmid: pU6 WNK1 Nter gRNA
• Parent Plasmid: pU6 gRNA BamHI
• Expressed: WNK1 Nter gRNA
• Genbank: NM_018979.3
• Species: Human

Sequence of Insert

gcgaaccgaccatgtctgg(cgg)

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48403
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Expresses WNK1 N-terminal guide RNA, 20mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets. Targets the ATG start codon in exon 1, PAM site situated on sense strand. Designed to introduce a GFP tag to WNK1 via CRISPR. To be used in conjunction with a WNK1 GFP donor plasmid and positives to be screened for by FACs.