cDNA Clone USP33

• Synonyms: VDU1
• Plasmid: pcDNA5D FRT/TO GFP USP33
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP USP33
• Genbank: NM_201626.2 GI:391738247 with insertion of residues RFVVSCVP after K551 (present in many other published sequences for USP33)
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGACAGGATCAAATTCACACATAACGATATTAACCTTAAAGGTGTTACCTCATTTTGAAAGTCTTGGGAAACAGGAAAAAATTCCTAACAAAATGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGAAATAACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAGGATTGTAAAGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATAGATGTTCATATGTTGGCTGTGGTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAGACAAAGCATTATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTATGCTTGCAGCAAAGAAGTATTTTTGGATAGGAAATTAGGAACTCAGCCTTCATTGCCTCATGTAAGACAACCTCACCAAATACAAGAAAACAGTGTCCAGGATTTTAAAATACCCAGTAATACAACATTAAAAACTCCTCTGGTTGCCGTATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGGGCCAGAGGTCTTACAGGTTTGAAAAATATTGGAAATACTTGTTACATGAATGCAGCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACACAGTTTTTTCTTGATTGTGGAGGACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTTATCTCAAACTAATGACAGAGCTGTGGCATAAAAGCAGGCCAGGATCTGTTGTGCCTACTACTCTGTTTCAAGGAATTAAAACTGTAAATCCAACATTTCGGGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATGGATTTGCTTCATGAAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCGCAAACCATAACCACTGAGGAGACAATGGAAGAAGACAAGAGCCAGTCGGATGTAGATTTTCAGTCTTGTGAATCTTGTAGCAACAGTGATAGAGCAGAAAATGAAAATGGCTCTAGATGCTTTTCTGAAGATAATAATGAAACAACAATGTTAATTCAGGATGATGAAAACAATTCAGAAATGTCAAAGGATTGGCAAAAAGAGAAGATGTGCAATAAGATTAATAAAGTAAATTCTGAAGGCGAATTTGATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCAGGAAACTGTCAAAGTGCAAATACACAGCAGAGCTTCAGAATATATCACTGATGTCCATTCGAATGACCTGTCTACACCACAGATCCTTCCATCAAATGAAGGTGTTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAGGCAATTTGTGGCCAGGATTGGCACCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAAAAAACAGCACAAGAAATACAGAAGTGTTATTTCAGACATATTTGATGGAACAATCATTAGTTCAGTGCAGTGTCTGACTTGTGACAGGGTGTCTGTAACCCTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAGACCTTGCTAAGCTGCATTCATCAAGTCATCCAACTTCTATAGTCAAAGCAGGATCATGTGGCGAAGCATATGCTCCACAAGGGTGGATAGCTTTTTTCATGGAATATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGCTGGTTTTGGGGTCCAGTAGTAACCTTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGATGAACTAAAAGGTGACAATATGTACAGTTGTGAAAAATGCAAAAAGTTGAGAAATGGAGTGAAGTTTTGTAAAGTACAAAACTTTCCTGAGATTTTGTGCATCCACCTTAAAAGATTCAGACATGAACTAATGTTTTCCACCAAAATCAGTACCCATGTTTCATTTCCGCTAGAAGGCTTGGATCTTCAGCCATTTCTTGCTAAGGATAGTCCAGCTCAAATTGTGACATATGATCTTCTGTCAGTCATTTGCCATCATGGAACTGCAAGTAGTGGACACTATATAGCCTACTGCCGAAACAATCTAAATAATCTCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCAGAATCTACTGTACAAAATGCAGAAGCTTACGTTCTTTTCTATAGGAAGAGCAGCGAAGAGGCACAAAAAGAGAGGAGAAGGATATCAAATTTATTGAACATAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGACAGTGGCTTAATAAATTTAAGACCTTTGCCGAACCTGGCCCTATTTCAAATAATGACTTTCTTTGTATTCATGGAGGTGTTCCTCCAAGAAAAGCTGGTTATATTGAAGACCTGGTTTTGATGCTGCCTCAGAACATTTGGGATAACCTATATAGCAGGTATGGTGGAGGACCAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAATTGAGGCGGAGAAAATTGAAAAAAGAAGAAAAACTGAATTGGAAATTTTTATTCGGGTAAAAAAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMTGSNS
HITILTLKVLPHFESLGKQEKIPNKMSAFRNHCPHLDSVGEITKEDLIQK
SLGTCQDCKVQGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVN
LTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQENSVQDFKIPSNT
TLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTCYMNAALQALSNC
PPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPTTLFQG
IKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEVEEDPQTITTEET
MEEDKSQSDVDFQSCESCSNSDRAENENGSRCFSEDNNETTMLIQDDENN
SEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHS
RASEYITDVHSNDLSTPQILPSNEGVNPRLSASPPKSGNLWPGLAPPHKK
AQSASPKRKKQHKKYRSVISDIFDGTIISSVQCLTCDRVSVTLETFQDLS
LPIPGKEDLAKLHSSSHPTSIVKAGSCGEAYAPQGWIAFFMEYVKRFVVS
CVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQ
NFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVT
YDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAE
AYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEP
GPISNNDFLCIHGGVPPRKAGYIEDLVLMLPQNIWDNLYSRYGGGPAVNH
LYICHTCQIEAEKIEKRRKTELEIFIRVKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5D FRT/TO GFP USP3356.88 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU49673
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: NM_201626.2 with insertion of residues RFVVSCVP after K551 (present in many other published sequences for USP33) (Matches EAX06371.1)
• Low Temp: No