cDNA Clone UBE2Z

• Synonyms: ubiquitin-conjugating enzyme E2Z (UBE2Z), Use1
• Plasmid: pET15b 6HIS C3 UBE2Z C286S
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-UBE2Z C286S
• Genbank: NM_023079.3 GI:150417998*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGAGAGTCCGACTGAGGAGGCGGCAACGGCGGGCGCCGGGGCGGCGGGCCCCGGGGCGAGCAGCGTTGCTGGTGTTGTTGGCGTTAGCG
GCAGCGGCGGCGGGTTCGGGCCGCCTTTCCTGCCGGATGTGTGGGCGGCGGCGGCGGCAGCGGGCGGGGCCGGGGGCCCGGGGAGCGGCCTGGCTCCGCT
GCCCGGGCTCCCGCCCTCAGCCGCTGCCCACGGGGCCGCGCTGCTTAGCCACTGGGACCCCACGCTCAGCTCCGACTGGGACGGCGAGCGCACCGCGCCG
CAGTGTCTACTCCGGATCAAGCGGGATATCATGTCCATTTATAAGGAGCCTCCTCCAGGAATGTTCGTTGTACCTGATACTGTTGACATGACTAAGATTC
ATGCATTGATCACAGGCCCATTTGACACTCCTTATGAAGGGGGTTTCTTCCTGTTCGTGTTTCGGTGTCCGCCCGACTATCCCATCCACCCACCTCGGGT
CAAACTGATGACAACGGGCAATAACACAGTGAGGTTTAACCCCAACTTCTACCGCAATGGGAAAGTCTGCTTGAGTATTCTAGGTACATGGACTGGACCT
GCCTGGAGCCCAGCCCAGAGCATCTCCTCAGTGCTCATCTCTATCCAGTCCCTGATGACTGAGAACCCCTATCACAATGAGCCCGGCTTTGAACAGGAGA
GACATCCAGGAGACAGCAAAAACTATAATGAATGTATCCGGCACGAGACCATCAGAGTTGCAGTCTGTGACATGATGGAAGGAAAGTGTCCCTGTCCTGA
ACCCCTACGAGGGGTGATGGAGAAGTCCTTTCTGGAGTATTACGACTTCTATGAGGTGGCCAGCAAAGATCGCCTGCACCTTCAAGGCCAAACTATGCAG
GACCCTTTTGGAGAGAAGCGGGGCCACTTTGACTACCAGTCCCTCTTGATGCGCCTGGGACTGATACGTCAGAAAGTGCTGGAGAGGCTCCATAATGAGA
ATGCAGAAATGGACTCTGATAGCAGTTCATCTGGGACAGAGACAGACCTTCATGGGAGCCTGAGGGTTTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAESPTEEAATAGAGAAGPGASSVAGVVGVSGSGGGFGPPFLPDVWAAAAAAGGAGGPGSGLAPLPGLPPSAAAHGA
ALLSHWDPTLSSDWDGERTAPQCLLRIKRDIMSIYKEPPPGMFVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRF
NPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMMEGKCPCPEPLRGVMEKSFLE
YYDFYEVASKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLERLHNENAEMDSDSSSSGTETDLHGSLRV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46568
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_023079.3 CDS: c846t (silent); ; Estimated MW of expressed protein : 40655 Daltons