cDNA Clone Ubiquitin

• Plasmid: pET3a-Ubiquitin-SUMO3 (M1-D15)-Ubiquitin -6HIS (wild type)
• Parent Plasmid: pET3a
• Expressed: Ubiquitin-SUMO3 M1-D15-Ubiquitin-6His
• Genbank: P62988
• Tag: 6His
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGTCCGAGGAGAAGCCCA
AGGAGGGTGTGAAGACAGAGAATGACATGCAGATCTTCGTGAAGACCCTG
ACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAA
TGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGA
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC
TACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGG
TGGGCATCATCACCATCACCATTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGGMSEEKPKEGVKTENDMQIFVKTLT
GKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDY
NIQKESTLHLVLRLRGGHHHHHH**

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24500
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: First 15 residues of SUMO3 inserted between the two ubiquitin ORFs