cDNA Clone USP45

• Plasmid: pcDNA5frtTO GFP NLS USP45 61-end C199A
• Parent Plasmid: pcDNA5 FRT/TO GFP NLS
• Expressed: GFP-NLS-USP45 61-end C199A
• Genbank: NP_001073950.1
• Tag: GFP
• Tag: NLS
• Species: Human

Sequence of Insert

AGATCTATGGTTTGCTCAGAATGTTTAAAAGAAAGAAGATTCTATGATGGGCAGCTAGTACTTACTTCTGATATTTGGTTGTGCCTCAAGTGTGGCTTCCAGGGATGTGGTAAAAACTCAGAAAGCCAACATTCATTGAAGCACTTTAAGAGTTCCAGAACAGAGCCCCATTGTATTATAATTAATCTGAGCACATGGATTATATGGTGTTATGAATGTGATGAAAAATTATCAACGCATTGTAATAAGAAGGTTTTGGCTCAGATAGTTGATTTTCTCCAGAAACATGCTTCTAAAACACAAACAAGTGCATTTTCTAGAATCATGAAACTTTGTGAAGAAAAATGTGAAACAGATGAAATACAGAAGGGAGGAAAATGCAGAAATTTATCTGTAAGAGGAATTACAAATTTAGGAAATACTGCCTTTTTTAATGCAGTCATGCAGAACTTGGCACAGACTTATACTCTTACTGATCTGATGAATGAGATCAAAGAAAGTAGTACAAAACTCAAGATTTTTCCTTCCTCAGACTCTCAGCTGGACCCATTGGTGGTGGAACTTTCAAGGCCTGGACCACTGACTTCTGCCTTGTTCCTGTTTCTTCACAGCATGAAGGAGACTGAAAAAGGACCACTTTCTCCTAAAGTTCTTTTTAATCAGCTTTGTCAGAAGGCACCTCGATTTAAAGATTTCCAGCAACAGGACAGTCAGGAGCTTCTTCATTATCTTCTGGATGCAGTGAGGACAGAAGAAACAAAGCGAATACAAGCTAGCATTCTAAAAGCATTTAACAACCCAACTACTAAAACTGCTGATGATGAAACTAGAAAAAAAGTCAAAGCATATGGAAAAGAAGGTGTGAAAATGAACTTCATAGATCGGATCTTTATTGGTGAATTAACTAGCACGGTCATGTGTGAAGAATGTGCAAATATCTCCACGGTGAAAGATCCATTCATTGATATTTCACTTCCTATAATAGAAGAAAGGGTTTCAAAACCTTTACTTTGGGGAAGAATGAATAAATATAGAAGTTTACGGGAGACAGATCATGATCGATACAGTGGCAATGTTACTATAGAAAATATTCATCAACCTAGAGCTGCCAAGAAGCATTCTTCATCTAAAGATAAGAGTCAACTAATTCATGACCGAAAATGTATTAGAAAATTGTCATCTGGAGAAACTGTCACATACCAGAAAAATGAAAACCTTGAAATGAATGGGGATTCTTTAATGTTTGCCAGCCTCATGAATTCTGAGTCACGTCTGAATGAAAGCCCTACTGATGACAGTGAAAAAGAAGCCAGCCATTCTGAAAGCAATGTTGATGCTGACAGTGAGCCTTCAGAATCTGAAAGTGCTTCAAAGCAGACTGGGCTGTTCAGATCCAGTAGTGGATCCGGTGTGCAGCCAGATGGACCCCTTTACCCTCTGTCAGCAGGTAAACTGCTGTACACCAAGGAGACTGACAGTGGTGATAAGGAAATGGCAGAAGCTATTTCTGAACTTCGTTTGAGCAGCACTGTAACTGGAGATCAAGATTTTGACAGAGAAAATCAGCCACTAAATATTTCAAATAATTTATGTTTTTTGGAGGGGAAGCATTTGAGGTCTTATAGTCCCCAAAATGCTTTTCAGACCCTTTCTCAGAGCTATATAACTACTTCTAAAGAATGTTCAATTCAGTCCTGTCTCTACCAGTTTACATCTATGGAATTACTAATGGGGAATAATAAGCTTCTATGTGAGAATTGTACTAAAAACAAACAGAAGTACCAAGAAGAAACCAGTTTTGCAGAAAAGAAAGTAGAAGGAGTTTATACTAATGCCAGGAAGCAATTGCTCATTTCTGCTGTTCCAGCTGTCCTAATTCTCCACCTGAAAAGATTTCATCAGGCTGGCTTGAGTCTTCGTAAAGTAAACAGACATGTAGATTTTCCACTTATGCTCGATTTAGCACCATTCTGCTCTGCTACTTGTAAGAATGCAAGTGTGGGAGATAAAGTTCTCTACGGTCTCTATGGCATAGTGGAACATAGTGGCTCGATGAGAGAAGGCCACTACACTGCTTATGTGAAAGTGAGAACACCCTCCAGGAAATTATCGGAACATAACACTAAAAAGAAAAATGTGCCTGGTTTGAAAGCGGCTGATAATGAATCAGCAGGCCAGTGGGTCCATGTTAGTGACACTTACTTACAGGTGGTTCCAGAATCAAGAGCACTTAGTGCACAAGCCTACCTTCTTTTCTATGAAAGAGTATTATAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKV
GSMVCSECLKERRFYDGQLVLTSDIWLCLKCGFQGCGKNSESQHSLKHFK
SSRTEPHCIIINLSTWIIWCYECDEKLSTHCNKKVLAQIVDFLQKHASKT
QTSAFSRIMKLCEEKCETDEIQKGGKCRNLSVRGITNLGNTAFFNAVMQN
LAQTYTLTDLMNEIKESSTKLKIFPSSDSQLDPLVVELSRPGPLTSALFL
FLHSMKETEKGPLSPKVLFNQLCQKAPRFKDFQQQDSQELLHYLLDAVRT
EETKRIQASILKAFNNPTTKTADDETRKKVKAYGKEGVKMNFIDRIFIGE
LTSTVMCEECANISTVKDPFIDISLPIIEERVSKPLLWGRMNKYRSLRET
DHDRYSGNVTIENIHQPRAAKKHSSSKDKSQLIHDRKCIRKLSSGETVTY
QKNENLEMNGDSLMFASLMNSESRLNESPTDDSEKEASHSESNVDADSEP
SESESASKQTGLFRSSSGSGVQPDGPLYPLSAGKLLYTKETDSGDKEMAE
AISELRLSSTVTGDQDFDRENQPLNISNNLCFLEGKHLRSYSPQNAFQTL
SQSYITTSKECSIQSCLYQFTSMELLMGNNKLLCENCTKNKQKYQEETSF
AEKKVEGVYTNARKQLLISAVPAVLILHLKRFHQAGLSLRKVNRHVDFPL
MLDLAPFCSATCKNASVGDKVLYGLYGIVEHSGSMREGHYTAYVKVRTPS
RKLSEHNTKKKNVPGLKAADNESAGQWVHVSDTYLQVVPESRALSAQAYL
LFYERVL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48244
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 61-end, C199A. 3 silent nucleotide changes as compared to the reference sequence; C>T 576 bp, A>T 579 bp and A>G 1590 bp.