cDNA Clone Ubiquitin

• Plasmid: pET24-Ubiquitin G75A G76A
• Parent Plasmid: pET24a original
• Expressed: untagged Ubiquitin G75A G76A
• Genbank: P62988*
• Tag: untagged
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGCTGCCTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRAA**

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU49488
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA); Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).; T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein. ; ; Estimated MW of expressed protein : 8565 Daltons