cDNA Clone Ubiquitin

• Plasmid: pET24-Ubiquitin S65D
• Parent Plasmid: pET24a original
• Expressed: untagged Ubiquitin S65D
• Genbank: P62988*
• Tag: untagged
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGGACACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKEDTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU49487
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA); (T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.); ; Estimated MW of expressed protein : 8565 Daltons