cDNA Clone ARNT

• Synonyms: Hif1beta
• Plasmid: pcDNA5 FRT/TO -GFP-ARNT
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-ARNT
• Genbank: Gene 405
• Species: Human

Sequence of Insert

ggatccATGGCGGCGACTACTGCCAACCCCGAAATGACATCAGATGTACCATCACT
GGGTCCAGCCATTGCCTCTGGAAACTCTGGACCTGGAATTCAAGGTGGAG
GAGCCATTGTCCAGAGGGCTATTAAGCGGCGACCAGGGCTGGATTTTGAT
GATGATGGAGAAGGGAACAGTAAATTTTTGAGGTGTGATGATGATCAGAT
GTCTAACGATAAGGAGCGGTTTGCCAGGTCGGATGATGAGCAGAGCTCTG
CGGATAAAGAGAGACTTGCCAGGGAAAATCACAGTGAAATTGAACGGCGG
CGACGGAACAAGATGACAGCCTACATCACAGAACTGTCAGATATGGTACC
CACCTGTAGTGCCCTGGCTCGAAAACCAGACAAGCTAACCATCTTACGCA
TGGCAGTTTCTCACATGAAGTCCTTGCGGGGAACTGGCAACACATCCACT
GATGGCTCCTATAAGCCGTCTTTCCTCACTGATCAGGAACTGAAACATTT
GATCTTGGAGGCAGCAGATGGCTTTCTGTTTATTGTCTCATGTGAGACAG
GCAGGGTGGTGTATGTCTCTGACTCCGTGACTCCTGTTTTGAACCAGCCA
CAGTCTGAATGGTTTGGCAGCACACTCTATGATCAGGTGCACCCAGATGA
TGTGGATAAACTTCGTGAGCAGCTTTCCACTTCAGAAAATGCCCTGACAG
GGCGTATCCTGGATCTAAAGACTGGAACAGTGAAAAAGGAAGGTCAGCAG
TCTTCCATGAGAATGTGTATGGGCTCAAGGAGATCGTTTATTTGCCGAAT
GAGGTGTGGCAGTAGCTCTGTGGACCCAGTTTCTGTGAATAGGCTGAGCT
TTGTGAGGAACAGATGCAGGAATGGACTTGGCTCTGTAAAGGATGGGGAA
CCTCACTTCGTGGTGGTCCACTGCACAGGCTACATCAAGGCCTGGCCCCC
AGCAGGTGTTTCCCTCCCAGATGATGACCCAGAGGCTGGCCAGGGAAGCA
AGTTTTGCCTAGTGGCCATTGGCAGATTGCAGGTAACTAGTTCTCCCAAC
TGTACAGACATGAGTAATGTTTGTCAACCAACAGAGTTCATCTCCCGACA
CAACATTGAGGGTATCTTCACTTTTGTGGATCACCGCTGTGTGGCTACTG
TTGGCTACCAGCCACAGGAACTCTTAGGAAAGAATATTGTAGAATTCTGT
CATCCTGAAGACCAGCAGCTTCTAAGAGACAGCTTCCAACAGGTAGTGAA
ATTAAAAGGCCAAGTGCTGTCTGTCATGTTCCGGTTCCGGTCTAAGAACC
AAGAATGGCTCTGGATGAGAACCAGCTCCTTTACTTTCCAGAACCCTTAC
TCAGATGAAATTGAGTACATCATCTGTACCAACACCAATGTGAAGAACTC
TAGCCAAGAACCACGGCCTACACTCTCCAACACAATCCAGAGGCCACAAC
TAGGTCCCACAGCTAATTTACCCCTGGAGATGGGCTCAGGACAGCTGGCA
CCCAGGCAGCAGCAACAGCAAACAGAATTGGACATGGTACCAGGAAGAGA
TGGACTGGCCAGCTACAATCATTCCCAGGTGGTTCAGCCTGTGACAACCA
CAGGACCAGAACACAGCAAGCCCCTTGAGAAGTCAGATGGTTTATTTGCC
CAGGATAGAGATCCAAGATTTTCAGAAATCTATCACAACATCAATGCGGA
TCAGAGTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAACAGCTAT
TCTCCCAGGGCAACACATTCCCTCCTACCCCCCGGCCGGCAGAGAATTTC
AGGAATAGTGGCCTAGCCCCTCCTGTAACCATTGTCCAGCCATCAGCTTC
TGCAGGACAGATGTTGGCCCAGATTTCCCGCCACTCCAACCCCACCCAAG
GAGCAACCCCAACTTGGACCCCTACTACCCGCTCAGGCTTTTCTGCCCAG
CAGGTGGCTACCCAGGCTACTGCTAAGACTCGTACTTCCCAGTTTGGTGT
GGGCAGCTTTCAGACTCCATCCTCCTTCAGCTCCATGTCCCTCCCTGGTG
CCCCAACTGCATCGCCTGGTGCTGCTGCCTACCCTAGTCTCACCAATCGT
GGATCTAACTTTGCTCCTGAGACTGGACAGACTGCAGGACAATTCCAGAC
ACGGACAGCAGAGGGTGTGGGTGTCTGGCCACAGTGGCAGGGCCAGCAGC
CTCATCATCGTTCAAGTTCTAGTGAGCAACATGTTCAACAACCGCCAGCA
CAGCAACCTGGCCAGCCTGAGGTCTTCCAGGAGATGCTGTCCATGCTGGG
AGATCAGAGCAACAGCTACAACAATGAAGAATTCCCTGATCTAACTATGT
TTCCCCCCTTTTCAGAATAGgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAATTA
NPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGN
SKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMT
AYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKP
SFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFG
STLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMC
MGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVV
HCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSN
VCQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQ
LLRDSFQQVVKLKGQVLSVMFRFRSKNQEWLWMRTSSFTFQNPYSDEIEY
IICTNTNVKNSSQEPRPTLSNTIQRPQLGPTANLPLEMGSGQLAPRQQQQ
QTELDMVPGRDGLASYNHSQVVQPVTTTGPEHSKPLEKSDGLFAQDRDPR
FSEIYHNINADQSKGISSSTVPATQQLFSQGNTFPPTPRPAENFRNSGLA
PPVTIVQPSASAGQMLAQISRHSNPTQGATPTWTPTTRSGFSAQQVATQA
TAKTRTSQFGVGSFQTPSSFSSMSLPGAPTASPGAAAYPSLTNRGSNFAP
ETGQTAGQFQTRTAEGVGVWPQWQGQQPHHRSSSSEQHVQQPPAQQPGQP
EVFQEMLSMLGDQSNSYNNEEFPDLTMFPPFSE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24380
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone harbours silent mutation at V189 (GTG to GTC) compared to NCBI ref.