cDNA Clone UBE2S

• Synonyms: E2-EPF, E2EPF, EPF5
• Plasmid: pMK-RQ UBE2S Cter GFP
• Parent Plasmid: pMK-RQ
• Genbank: ENSG00000108106
• Species: Human

Sequence of Insert

gcggccgcTGGCCTCTGAGGGAGACAGGAGCAAAGATGAGGGAGGATGCAGCATAGGTGGGGCCTGGATCTGCAGCTGAGTTGGGAGCAGAAGGAACAGCCAGTGCGGAGGCCACCCTCTTGTGGAGGCCTGGGGGTCCCTGTAGGTTGCCCGACCCACTGTGACCCTGAGCCTCTTCCCACAGACCATCAAGTGCCTGCTGATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAGGCGGGCCGCCTGCTCTTGGAGAACTACGAGGAGTATGCGGCTCGGGCCCGTCTGCTCACAGAGATCCACGGGGGCGCCGGCGGGCCCAGCGGCAGGGCCGAAGCCGGTCGGGCCCTGGCCAGTGGCACTGAAGCTTCCTCCACCGACCCTGGGGCCCCAGGGGGCCCGGGAGGGGCTGAGGGTCCCATGGCCAAGAAGCATGCTGGCGAGCGCGATAAGAAGCTGGCGGCCAAGAAAAAGACGGACAAGAAGCGGGCtCTaCGaCGaCTcGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAGTGGGCTCTCTTCCTCCTTCCACCGTGACCCCAACCTCTCCTGTCCCCTCCCTCCAACTCTGTCTCTAAGTTATTTAAATTATGGCTGGGGTCGGGGAGGGTACAGGGGGCACTGGGACCTGGATTTGTTTTTCTAAATAAAGTTGGAAAAGCAGCTTCTGTGTCTCACAGATGCCCCAGGACCGCTGCATGGTGCCATGTGGCTCTGGGAGTTTGGCTCCCCTGCACAGGCCCTGGGTCCTTGTAAAGCCTCATGGACTGCAGCAGTGGGTCCATCTAGCTCACCAGCCTTGCTGTTTGAGGTTTGGGTCCACCTCTTTGTTCTGTGGAGGTAGAGCGGTCACAACCCCTCTTTGGAGTCAGATTTCCAATCTAAAATGGGGCGAGGACCTGGAGCTGCTCCTGGTGGCCTGAGCGGCACTGCCCTATACTTCTCCCTCTAGAATAATGGCCACGTTGGTGTGGCAGTGGTGTTCCTGCTCAGGACGGCCAGGTGCCAGCggccgc

Amino Acid Sequence

MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGP
EGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGANGEICVNVLK
RDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARL
LTEIHGGAGGPSGRAEAGRALASGTEASSTDPGAPGGPGGAEGPMAKKHA
GERDKKLAAKKKTDKKRALRRLVSKGEELFTGVVPILVELDGDVNGHKFS
VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHM
KQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGI
DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQL
ADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAG
ITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48135
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP upstream of the UBE2S stop codon. Targets last coding exon (exon 4). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Sequence of cassette depicts the expressed UBE2S-GFP fusion.