cDNA Clone TTBK2

• Synonyms: KIAA0847, SCA11, TTBK
• Plasmid: pcDNA3.1 Hygro TTBK2 D163A
• Parent Plasmid: pcDNA3.1 Hygro
• Expressed: TTBK2 D163A
• Genbank: NM_173500.3
• Species: Human

Sequence of Insert

GGATCCGCCATGAGTGGGGGAGGAGAGCAGCCGGATATCCTGAGTGTTGGAATCCTAGTGAAAGAAAGATGGAAAGTGTTGAGAAAGATTGGGGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAAAATGTTGCACTGAAGGTGGAATCAGCTCAACAACCAAAACAAGTTCTGAAAATGGAAGTTGCTGTTTTGAAAAAGCTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGGAATGATCGATTCAACTATGTGGTCATGCAGTTGCAGGGTCGGAATCTGGCAGATCTTCGCCGTAGCCAGTCCCGAGGCACATTCACCATTAGTACCACTCTCCGGCTGGGTAGACAGATTTTGGAGTCTATTGAAAGCATTCATTCTGTGGGATTCTTGCATCGAGACATCAAACCGTCGAACTTCGCTATGGGTCGCTTTCCTAGTACATGTAGGAAATGTTACATGCTTGCTTTTGGCTTGGCTCGACAATTTACCAATTCCTGTGGTGACGTCAGACCACCTCGAGCTGTGGCAGGTTTTCGAGGGACAGTTCGTTATGCATCAATCAACGCACATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCCTTATTCTACATGTTGGTGGAGTTTGTGGTTGGTCAGCTGCCCTGGAGAAAAATAAAGGACAAGGAGCAAGTAGGCTCTATTAAGGAGAGATATGACCACAGGCTCATGTTGAAACATCTCCCTCCAGAATTCAGCATCTTTCTAGACCATATCTCTTCTTTGGATTATTTTACAAAACCAGACTACCAGCTTCTTACATCCGTGTTTGACAATAGCATCAAGACTTTTGGAGTAATTGAGAGTGACCCTTTTGACTGGGAGAAGACTGGAAATGATGGCTCCCTAACAACCACCACTACTTCTACCACCCCTCAGTTGCACACTCGCTTGACCCCTGCTGCAATTGGAATTGCCAATGCTACTCCCATCCCTGGAGACTTGCTTCGAGAAAATACAGATGAGGTATTTCCAGATGAACAGCTTAGCGATGGAGAAAATGGCATCCCTGTTGGTGTGTCACCAGATAAATTGCCTGGATCTCTGGGACACCCCCGTCCCCAGGAGAAGGATGTTTGGGAAGAGATGGATGCCAACAAAAACAAGATAAAGCTTGGAATTTGTAAGGCTGCTACTGAAGAGGAGAACAGCCATGGCCAGGCAAATGGTCTTCTCAATGCTCCAAGCCTTGGGTCACCAATTCGTGTCCGCTCAGAGATTACTCAGCCAGACAGAGATATTCCACTGGTGCGAAAGTTACGTTCCATTCACAGCTTTGAGCTGGAAAAACGTCTGACCCTGGAGCCAAAGCCAGACACTGACAAGTTCCTTGAGACCTGCCTGGAGAAAATGCAGAAAGATACCAGTGCAGGAAAAGAATCTATTCTCCCTGCTCTGCTGCATAAGCCTTGCGTTCCTGCTGTGTCCCGTACTGACCACATCTGGCACTATGATGAAGAATATCTTCCAGATGCCTCCAAGCCTGCTTCTGCCAACACCCCTGAGCAGGCAGATGGTGGTGGCAGCAATGGATTTATAGCTGTTAACCTGAGCTCTTGCAAGCAAGAAATTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAGGACCTTCAGGATTTTAGGACAAATGAGGCTGTAGGACATAAAACAACTGGAAGTCCTTCTGATGAGGAGCCTGAAGTACTTCAAGTCCTGGAGGCATCACCTCAAGATGAAAAGCTCCAGTTAGGTCCTTGGGCAGAAAATGATCATTTAAAGAAGGAAACCTCAGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCTACTGCTGCTTCAGAACAATATACAGATAGGCTGGAACTCCAGCCTGGAGCTGCTAGTCAGTTTATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGGCAGAAGGACCCCTTACAGCGATTACAATTCCTAGACCTTCTGTGGCATCTACACAGTCAACTTCAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAAAGATCTTCAGCCCATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAAACTTCTCTGGCTTGGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGCAGAACAGATTTGGGGCTTCAGATAGATCACATTGGTCATGACATGTTACCCAACATTAGAGAAAGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTCCTGATCATAATAGACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAAACTGAAGAGAAAAGCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTTAAGTAGAGGGCAGCATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGGTAATTGTGGAAAAGGATCACTCAGCTACTACTGAACCTCTTGATGTGACAAAAACACAGACTTTTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGATAATGAAGCTTCTGACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTGACCCACATGTTGAAGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAATAAGATATCTAAGGATGATGACATCATGAGTGAAGACTTGCCAGGTCATCAAGGAGACCTCTCTACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCACCCCTAGAAATGGAGAACTATTTCATTGTGTTTCAGAGAATGAACATGGTGCCCCAACCCGGAAGGATATGGTTAGGTCATCCTTTGTAACTAGACACAGCCGAATCCCTGTTTTAGCACAAGAGATAGACTCAACTTTGGAATCATCCTCTCCAGTTTCTGCAAAAGAAAAGCTCCTCCAAAAGAAAGCCTATCAGCCAGACCTAGTCAAGCTTCTGGTGGAAAAAAGACAATTCAAGTCCTTCCTTGGCGACCTCTCAAGTGCCTCTGATAAATTGCTAGAGGAGAAACTAGCTACTGTTCCTGCTCCCTTTTGTGAGGAGGAAGTGCTCACTCCCTTTTCAAGACTGACAGTAGATTCTCACCTGAGTAGGTCAGCTGAAGATAGCTTTCTGTCACCCATCATCTCCCAGTCTAGAAAGAGCAAAATTCCAAGGCCAGTTTCATGGGTCAACACAGATCAGGTCAATAGCTCAACTTCGTCTCAGTTCTTTCCTCGGCCACCACCAGGAAAGCCACCCACGAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTCCTAGGGAGTAGTAACTCCGACTCAGACCTTTTCTCCCGCCTGGCCCAAATTCTTCAAAATGGATCTCAGAAACCCCGGAGCACTACTCAGTGCAAGAGTCCAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTGTCCCTCGCAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGCACGTCTAGTTCCTCACCATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTTCGTCCCCACATCTGGGGAGAAGCAAGTCACCTCCCAGCCACTCAGGATCTTCCTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAATGGCCTGAAAGGCTCCGGCAGCCTCCACCACCACTCAGCCAGCACTAAAACCCCCCAAGGGAAGAGTAAGCCAGCCAGTAAACTCAGCAGATAGCGGCCGC

Amino Acid Sequence

MSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALK
VESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRN
LADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMG
RFPSTCRKCYMLAFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRN
REMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKH
LPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKT
GNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDE
QLSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDANKNKIKLGICKA
ATEEENSHGQANGLLNAPSLGSPIRVRSEITQPDRDIPLVRKLRSIHSFE
LEKRLTLEPKPDTDKFLETCLEKMQKDTSAGKESILPALLHKPCVPAVSR
TDHIWHYDEEYLPDASKPASANTPEQADGGGSNGFIAVNLSSCKQEIDSK
EWVIVDKEQDLQDFRTNEAVGHKTTGSPSDEEPEVLQVLEASPQDEKLQL
GPWAENDHLKKETSGVVLALSAEGPPTAASEQYTDRLELQPGAASQFIAA
TPTSLMEAQAEGPLTAITIPRPSVASTQSTSGSFHCGQQPEKKDLQPMEP
TVELYSPRENFSGLVVTEGEPPSGGSRTDLGLQIDHIGHDMLPNIRESNK
SQDLGPKELPDHNRLVVREFENLPGETEEKSILLESDNEDEKLSRGQHCI
EISSLPGDLVIVEKDHSATTEPLDVTKTQTFSVVPNQDKNNEIMKLLTVG
TSEISSRDIDPHVEGQIGQVAEMQKNKISKDDDIMSEDLPGHQGDLSTFL
HQEGKREKITPRNGELFHCVSENEHGAPTRKDMVRSSFVTRHSRIPVLAQ
EIDSTLESSSPVSAKEKLLQKKAYQPDLVKLLVEKRQFKSFLGDLSSASD
KLLEEKLATVPAPFCEEEVLTPFSRLTVDSHLSRSAEDSFLSPIISQSRK
SKIPRPVSWVNTDQVNSSTSSQFFPRPPPGKPPTRPGVEARLRRYKVLGS
SNSDSDLFSRLAQILQNGSQKPRSTTQCKSPGSPHNPKTPPKSPVVPRRS
PSASPRSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPPSHSGSSSS
RRSCQQEHCKPSKNGLKGSGSLHHHSASTKTPQGKSKPASKLSR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48076
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D163A. Silent A>C change at nucleotide 3654.