cDNA Clone USP36

• Synonyms: DUB1
• Plasmid: pcDNA5 FRT/TO.GFP.DU USP36 C131A
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-USP36 C131A
• Genbank: NM_025090.3 GI:122114650 with silent change c1863t
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCCAATAGTGGATAAGTTGAAGGAGGCCCTGAAACCCGGCCGCAAGGACTCGGCTGATGATGGAGAACTGGGGAAGCTTCTTGCCTCCTCTGCCAAGAAGGTCCTTTTACAGAAAATCGAGTTCGAGCCAGCCAGCAAGAGCTTCTCCTACCAGCTGGAGGCCTTAAAGAGCAAATATGTGTTGCTCAACCCCAAAACAGAGGGAGCTAGTCGCCACAAGAGTGGAGATGACCCACCGGCCAGGAGACAGGGCAGTGAGCACACGTATGAGAGCTGTGGTGACGGAGTCCCAGCCCCGCAGAAAGTGCTTTTCCCCACGGAGCGACTGTCTCTGAGGTGGGAGCGGGTCTTCCGCGTGGGCGCAGGACTCCACAACCTTGGCAACACCGCCTTTCTCAATGCCACCATCCAGTGCTTGACCTACACACCACCTCTAGCCAACTACCTGCTCTCCAAGGAGCATGCTCGCAGCTGCCACCAGGGAAGCTTCTGCATGCTGTGTGTCATGCAGAACCACATTGTCCAGGCCTTCGCCAACAGCGGCAACGCCATCAAGCCCGTCTCCTTCATCCGAGACCTGAAAAAGATCGCCCGACACTTCCGCTTTGGGAACCAGGAGGACGCGCATGAGTTCCTGCGGTACACCATCGACGCCATGCAGAAAGCCTGCCTGAATGGCTGTGCCAAGTTGGATCGTCAAACGCAGGCTACTACCTTGGTCCATCAAATTTTTGGAGGGTATCTCAGATCACGCGTGAAGTGCTCCGTGTGCAAGAGCGTCTCGGACACCTACGACCCCTACTTGGACGTCGCGCTGGAGATCCGGCAAGCTGCGAATATTGTGCGTGCTCTGGAACTTTTTGTGAAAGCAGATGTCCTGAGTGGAGAGAATGCCTACATGTGTGCTAAATGCAAGAAGAAGGTTCCAGCCAGCAAGCGCTTCACCATCCACAGAACATCCAACGTCTTAACCCTTTCCCTCAAGCGCTTTGCCAACTTCAGCGGGGGGAAGATCACCAAGGATGTAGGCTATCCGGAATTCCTCAACATACGTCCGTATATGTCCCAGAATAATGGTGATCCTGTCATGTATGGACTCTATGCTGTCCTGGTGCACTCGGGCTACAGCTGCCATGCCGGGCACTATTACTGCTACGTGAAGGCAAGCAATGGACAGTGGTACCAGATGAATGATTCCTTGGTCCATTCCAGCAACGTCAAGGTGGTTCTGAACCAGCAGGCCTACGTGCTGTTCTATCTGCGAATTCCAGGCTCTAAGAAAAGTCCCGAGGGCCTCATCTCCAGGACAGGCTCCTCCTCCCTTCCCGGCCGCCCGAGTGTGATTCCAGATCACTCCAAGAAGAACATCGGCAATGGGATTATTTCCTCCCCACTGACTGGAAAGCGACAAGACTCTGGGACGATGAAGAAGCCGCACACCACTGAAGAGATTGGTGTGCCCATATCCAGGAATGGCTCCACCCTGGGCCTGAAGTCCCAGAACGGCTGCATTCCTCCAAAGCTGCCCTCGGGGTCCCCTTCCCCCAAACTCTCCCAGACACCCACACACATGCCAACCATCCTAGACGACCCTGGAAAGAAGGTGAAGAAGCCAGCTCCTCCACAGCACTTTTCCCCCAGAACTGCTCAGGGGCTGCCTGGGACCAGCAACTCGAATAGCAGCAGATCTGGGAGCCAAAGGCAGGGCTCCTGGGACAGCAGGGATGTTGTCCTCTCTACCTCACCTAAGCTCCTGGCTACAGCCACTGCCAACGGGCATGGGCTGAAGGGGAACGACGAGAGCGCTGGCCTCGACAGGAGGGGCTCCAGCAGCTCCAGCCCAGAGCACTCGGCCAGCAGTGACTCCACCAAGGCCCCCCAGACCCCCAGGAGTGGAGCGGCCCATCTCTGCGATTCTCAGGAAACGAACTGTTCCACCGCTGGCCACTCCAAAACGCCGCCAAGTGGAGCAGATTCTAAGACGGTGAAGCTGAAGTCCCCTGTCCTGAGCAACACCACCACTGAGCCTGCAAGCACCATGTCTCCTCCACCAGCCAAAAAACTGGCCCTTTCTGCCAAGAAGGCCAGCACCCTGTGGAGGGCGACCGGCAATGACCTCCGTCCACCTCCCCCCTCACCATCCTCCGACCTCACCCACCCCATGAAAACCTCTCACCCCGTCGTTGCCTCCACTTGGCCCGTCCATAGAGCCAGGGCTGTGTCACCTGCTCCCCAATCATCCAGCCGCCTGCAACCCCCCTTCAGCCCCCACCCCACATTGCTGTCCAGTACCCCCAAGCCCCCAGGGACGTCAGAACCACGGAGCTGCTCCTCCATCTCGACGGCGCTGCCTCAGGTCAACGAGGACCTTGTGTCTCTTCCACACCAGTTGCCAGAGGCCAGTGAGCCCCCCCAGAGCCCCTCTGAGAAGAGGAAAAAGACCTTTGTGGGAGAGCCGCAGAGGCTGGGCTCAGAGACGCGCCTCCCACAGCACATCAGGGAGGCCACTGCGGCTCCCCACGGGAAGAGGAAGAGGAAGAAGAAGAAGCGCCCGGAGGACACAGCTGCCAGCGCCCTGCAGGAGGGGCAGACACAGAGACAGCCTGGGAGCCCCATGTACAGGAGGGAGGGCCAGGCACAGCTGCCCGCTGTCAGACGGCAGGAAGATGGCACACAGCCACAGGTGAATGGCCAGCAGGTGGGATGTGTTACGGACGGCCACCACGCGAGCAGCAGGAAGCGGAGGAGGAAAGGAGCAGAAGGTCTTGGTGAAGAAGGCGGCCTGCACCAGGACCCACTTCGGCACAGCTGCTCTCCCATGGGTGATGGTGATCCAGAGGCCATGGAAGAGTCTCCAAGGAAAAAGAAAAAGAAAAAAAGAAAGCAGGAGACACAGCGGGCAGTAGAAGAGGATGGGCATCTCAAATGCCCAAGGAGTGCCAAGCCCCAAGATGCTGTTGTCCCCGAGTCCAGCAGCTGCGCACCATCCGCGAATGGCTGGTGTCCTGGGGACCGCATGGGGCTGAGCCAGGCCCCTCCTGTGTCTTGGAATGGAGAGCGGGAGTCTGATGTGGTCCAGGAACTGCTCAAATACTCATCTGATAAAGCTTACGGGAGAAAAGTTCTGACCTGGGATGGCAAGATGTCGGCGGTCAGTCAGGATGCTATTGAAGACAGCAGACAGGCCCGGACTGAGACCGTGGTTGATGACTGGGACGAAGAGTTTGACCGAGGGAAGGAAAAGAAAATTAAAAAATTTAAGAGAGAGAAGAGGAGAAACTTCAACGCCTTCCAGAAACTTCAGACTCGACGGAACTTCTGGTCTGTGACTCACCCAGCAAAGGCTGCCAGCCTCAGCTATCGCCGCTAAGGGCGAATTCCACATTGGTCGCTGCAGCCCGGGGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPIVDK
LKEALKPGRKDSADDGELGKLLASSAKKVLLQKIEFEPASKSFSYQLEAL
KSKYVLLNPKTEGASRHKSGDDPPARRQGSEHTYESCGDGVPAPQKVLFP
TERLSLRWERVFRVGAGLHNLGNTAFLNATIQCLTYTPPLANYLLSKEHA
RSCHQGSFCMLCVMQNHIVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQ
EDAHEFLRYTIDAMQKACLNGCAKLDRQTQATTLVHQIFGGYLRSRVKCS
VCKSVSDTYDPYLDVALEIRQAANIVRALELFVKADVLSGENAYMCAKCK
KKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMS
QNNGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSN
VKVVLNQQAYVLFYLRIPGSKKSPEGLISRTGSSSLPGRPSVIPDHSKKN
IGNGIISSPLTGKRQDSGTMKKPHTTEEIGVPISRNGSTLGLKSQNGCIP
PKLPSGSPSPKLSQTPTHMPTILDDPGKKVKKPAPPQHFSPRTAQGLPGT
SNSNSSRSGSQRQGSWDSRDVVLSTSPKLLATATANGHGLKGNDESAGLD
RRGSSSSSPEHSASSDSTKAPQTPRSGAAHLCDSQETNCSTAGHSKTPPS
GADSKTVKLKSPVLSNTTTEPASTMSPPPAKKLALSAKKASTLWRATGND
LRPPPPSPSSDLTHPMKTSHPVVASTWPVHRARAVSPAPQSSSRLQPPFS
PHPTLLSSTPKPPGTSEPRSCSSISTALPQVNEDLVSLPHQLPEASEPPQ
SPSEKRKKTFVGEPQRLGSETRLPQHIREATAAPHGKRKRKKKKRPEDTA
ASALQEGQTQRQPGSPMYRREGQAQLPAVRRQEDGTQPQVNGQQVGCVTD
GHHASSRKRRRKGAEGLGEEGGLHQDPLRHSCSPMGDGDPEAMEESPRKK
KKKKRKQETQRAVEEDGHLKCPRSAKPQDAVVPESSSCAPSANGWCPGDR
MGLSQAPPVSWNGERESDVVQELLKYSSDKAYGRKVLTWDGKMSAVSQDA
IEDSRQARTETVVDDWDEEFDRGKEKKIKKFKREKRRNFNAFQKLQTRRN
FWSVTHPAKAASLSYRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49088
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee