cDNA Clone UBQLN1

• Synonyms: DA41; DSK2; PLIC-1; UBQN; XDRP1
• Plasmid: pcDNA5D FRT/TO GST C3 Halo Thr UBQLN1
• Parent Plasmid: pcDNA5D FRT/TO GST 3C Halo Thrombin
• Expressed: GST-C3-Halo-Thrombin UBQLN1
• Genbank: NM_013438.4 GI:194328681 with change a1221g (silent at Leu407)
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccATGGCCGAGAGTGGTGAAAGCGGCGGTCCTCCGGGCTCCCAGGA TAGCGCCGCCGGAGCCGAAGGTGCTGGCGCCCCCGCGGCCGCTGCCTCCG CGGAGCCCAAAATCATGAAAGTCACCGTGAAGACCCCGAAGGAAAAGGAG GAATTCGCCGTGCCCGAGAATAGCTCCGTCCAGCAGTTTAAGGAAGAAAT CTCTAAACGTTTTAAATCACATACTGACCAACTTGTGTTGATATTTGCTG GAAAAATTTTGAAAGATCAAGATACCTTGAGTCAGCATGGAATTCATGAT GGACTTACTGTTCACCTTGTCATTAAAACACAAAACAGGCCTCAGGATCA TTCAGCTCAGCAAACAAATACAGCTGGAAGCAATGTTACTACATCATCAA CTCCTAATAGTAACTCTACATCTGGTTCTGCTACTAGCAACCCTTTTGGT TTAGGTGGCCTTGGGGGACTTGCAGGTCTGAGTAGCTTGGGTTTGAATAC TACCAACTTCTCTGAACTACAGAGTCAGATGCAGCGACAACTTTTGTCTA ACCCTGAAATGATGGTCCAGATCATGGAAAATCCCTTTGTTCAGAGCATG CTCTCAAATCCTGACCTGATGAGACAGTTAATTATGGCCAATCCACAAAT GCAGCAGTTGATACAGAGAAATCCAGAAATTAGTCATATGTTGAATAATC CAGATATAATGAGACAAACGTTGGAACTTGCCAGGAATCCAGCAATGATG CAGGAGATGATGAGGAACCAGGACCGAGCTTTGAGCAACCTAGAAAGCAT CCCAGGGGGATATAATGCTTTAAGGCGCATGTACACAGATATTCAGGAAC CAATGCTGAGTGCTGCACAAGAGCAGTTTGGTGGTAATCCATTTGCTTCC TTGGTGAGCAATACATCCTCTGGTGAAGGTAGTCAACCTTCCCGTACAGA AAATAGAGATCCACTACCCAATCCATGGGCTCCACAGACTTCCCAGAGTT CATCAGCTTCCAGCGGCACTGCCAGCACTGTGGGTGGCACTACTGGTAGT ACTGCCAGTGGCACTTCTGGGCAGAGTACTACTGCGCCAAATTTGGTGCC TGGAGTAGGAGCTAGTATGTTCAACACACCAGGAATGCAGAGCTTGTTGC AACAAATAACTGAAAACCCACAACTGATGCAAAACATGTTGTCTGCCCCC TACATGAGAAGCATGATGCAGTCACTgAGCCAGAATCCTGACCTTGCTGC ACAGATGATGCTGAATAATCCCCTATTTGCTGGAAATCCTCAGCTTCAAG AACAAATGAGACAACAGCTCCCAACTTTCCTCCAACAAATGCAGAATCCT GATACACTATCAGCAATGTCAAACCCTAGAGCAATGCAGGCCTTGTTACA GATTCAGCAGGGTTTACAGACATTAGCAACGGAAGCCCCGGGCCTCATCC CAGGGTTTACTCCTGGCTTGGGGGCATTAGGAAGCACTGGAGGCTCTTCG GGAACTAATGGATCTAACGCCACACCTAGTGAAAACACAAGTCCCACAGC AGGAACCACTGAACCTGGACATCAGCAGTTTATTCAGCAGATGCTGCAGG CTCTTGCTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAG CAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAA CTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAA GGTTACTGGGCTCCCAGCCATCATAGagatct

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMAESGESGGPPGSQ
DSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEE
ISKRFKSHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQD
HSAQQTNTAGSNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLN
TTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQ
MQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLES
IPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRT
ENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTAPNLV
PGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLA
AQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALL
QIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPT
AGTTEPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLSAMGFLNREA
NLQALIATGGDINAAIERLLGSQPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49020
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental Plasmid DU49011