cDNA Clone TTBK1

• Synonyms: BDTK
• Plasmid: pBABE H GFP TTBK1 D176A (KD)
• Parent Plasmid: pBabeD hygro GFP
• Expressed: GFP-TTBK1 D176A (KD)
• Genbank: NM_032538.1
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCAGTGCCTAGCGGCAGCTCTTAAGGACGAAACCAACATGAGTGGGGGAGGGGAGCAGGCCGACATCCTGCCGGCCAACTACGTGGTCAAAGATCGCTGGAAGGTGCTGAAAAAGATCGGGGGCGGGGGCTTCGGTGAGATCTATGAGGCCATGGACCTGCTGACCAGGGAGAACGTGGCTCTCAAGGTGGAGTCAGCCCAACAGCCTAAGCAGGTCCTCAAGATGGAGGTGGCTGTGCTCAAAAAGCTTCAAGGCAAGGACCACGTGTGCAGGTTCATTGGCTGTGGCCGTAACGAGAAGTTTAACTATGTGGTGATGCAGCTCCAGGGCCGGAACCTGGCTGACCTGCGCCGCAGCCAGCCAAGGGGCACTTTCACGTTGAGTACCACACTGCGCCTGGGCAAGCAGATCCTGGAGTCCATTGAAGCCATTCACTCCGTGGGCTTCCTGCACCGTGACATCAAGCCGTCCAACTTTGCCATGGGCCGGCTGCCCTCGACCTACAGGAAGTGCTACATGTTGGCCTTTGGGCTAGCCAGGCAGTACACTAACACCACTGGGGACGTCCGGCCTCCTCGGAATGTGGCCGGGTTCCGGGGGACTGTCCGCTACGCCTCGGTCAATGCTCACAAGAACCGGGAGATGGGCCGCCATGATGACCTGTGGTCCCTCTTCTACATGCTGGTGGAGTTTGCTGTGGGCCAGCTGCCCTGGAGGAAGATCAAAGACAAGGAGCAGGTGGGGATGATCAAAGAGAAATACGAGCACCGGATGCTACTGAAGCACATGCCATCCGAGTTTCATCTCTTCCTGGACCACATTGCCAGCCTCGACTACTTCACCAAGCCCGATTACCAGTTGATCATGTCAGTGTTTGAGAACAGCATGAAGGAGCGGGGCATTGCCGAGAACGAGGCCTTTGACTGGGAGAAGGCAGGCACTGATGCCCTCCTGTCCACGAGCACCTCCACCCCACCCCAGCAGAACACGCGACAGACCGCAGCCATGTTTGGAGTGGTCAATGTGACACCAGTGCCAGGGGATCTGCTGAGGGAAAATACCGAAGACGTGCTCCAGGGGGAACATCTGTCCGACCAGGAAAACGCTCCACCAATCCTGCCTGGAAGACCATCTGAGGGCCTCGGACCTAGTCCACACCTGGTGCCACATCCTGGAGGACCAGAAGCAGAAGTGTGGGAGGAAACCGACGTGAACCGAAATAAGCTGAGGATCAACATTGGCAAAAGCCCTTGCGTGGAGGAAGAGCAGTCCAGGGGAATGGGTGTGCCAAGCTCCCCTGTCAGAGCTCCCCCTGATAGCCCAACCACACCCGTGCGCTCCCTGCGATACAGGAGAGTCAATTCTCCTGAAAGTGAGCGACTCTCTACAGCAGACGGAAGGGTGGAACTGCCAGAGCGGCGCAGCCGAATGGATCTCCCTGGCAGTCCATCACGACAGGCCTGTTCTAGTCAGCCTGCTCAGATGCTGTCCGTGGACACTGGTCACGCCGATAGGCAGGCTTCTGGCAGAATGGACGTGAGCGCCTCCGTCGAACAGGAGGCACTGTCAAACGCCTTCAGAAGCGTGCCACTCGCCGAAGAGGAAGACTTTGATAGCAAGGAGTGGGTGATCATTGACAAGGAAACCGAGCTGAAAGATTTCCCACCAGGAGCAGAGCCATCTACAAGTGGAACTACCGATGAGGAACCTGAGGAACTGCGGCCACTCCCAGAGGAAGGAGAGGAACGAAGGAGACTGGGTGCAGAGCCTACAGTGCGCCCACGAGGCAGGAGCATGCAGGCACTCGCCGAGGAAGACCTGCAGCACCTCCCTCCACAGCCACTCCCACCTCAGCTGTCCCAGGGAGATGGACGCTCAGAGACCAGCCAGCCACCCACACCCGGTTCACCTAGCCACTCCCCACTGCATTCAGGACCTCGGCCACGGCGCCGAGAGTCCGACCCAACCGGACCACAGCGACAGGTGTTCTCCGTCGCTCCTCCATTTGAGGTCAATGGACTGCCAAGGGCAGTCCCTCTCAGCCTGCCATACCAGGATTTCAAGAGAGACCTGTCCGATTATAGAGAGCGAGCACGACTGCTCAACCGGGTGAGGAGAGTCGGATTTTCTCATATGCTGCTCACAACTCCTCAGGTGCCACTGGCACCCGTCCAGCCTCAGGCCAATGGGAAAGAGGAGGAAGAAGAGGAAGAGGAAGACGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGCCGCTGCAGCCGTGGCCCTCGGAGAGGTCCTGGGGCCTAGAAGCGGTTCAAGCTCCGAAGGCAGCGAGAGATCTACTGACAGGAGCCAGGAGGGAGCTCCAAGCACCCTGCTCGCAGACGATCAGAAGGAGTCCCGAGGCAGGGCCTCTATGGCTGACGGAGATCTGGAGCCTGAAGAGGGGTCTAAAACTCTCGTGCTGGTCAGTCCAGGCGACATGAAGAAATCACCCGTGACCGCAGAGCTCGCACCCGACCCTGATCTGGGAACTCTCGCTGCACTGACCCCACAGCACGAGAGGCCACAGCCAACAGGTAGTCAGCTGGACGTGAGCGAACCTGGCACTCTCTCTAGTGTCCTGAAGTCCGAGCCAAAACCACCTGGACCTGGTGCTGGACTGGGAGCAGGGACTGTGACCACAGGAGTCGGGGGTGTGGCTGTCACATCAAGCCCTTTCACTAAGGTGGAACGCACCTTTGTCCACATCGCCGAGAAAACCCATCTGAACGTGATGTCCTCTGGCGGACAGGCTCTCAGGAGCGAAGAGTTTTCCGCAGGAGGTGAACTCGGACTGGAGCTCGCTTCCGACGGAGGAGCAGTGGAAGAGGGAGCTAGAGCACCCCTGGAGAACGGGCTGGCCCTCTCTGGACTCAATGGGGCTGAAATTGAGGGCAGTGCCCTGTCAGGAGCTCCACGAGAAACACCTTCCGAGATGGCTACTAACTCTCTCCCAAATGGACCCGCCCTGGCTGATGGACCTGCCCCAGTGAGCCCACTGGAACCATCCCCTGAGAAGGTCGCAACCATCAGCCCTCGGCGCCATGCTATGCCAGGCTCTAGACCCCGGAGTCGCATTCCTGTGCTGCTCAGTGAAGAGGACACAGGGTCAGAACCATCTGGTAGTCTGTCAGCCAAGGAGCGGTGGTCTAAACGAGCAAGGCCTCAGCAGGATCTCGCCCGCCTGGTGATGGAGAAGAGGCAGGGAAGACTGCTCCTGAGGCTGGCTAGCGGGGCAAGTTCAAGCTCCTCTGAAGAGCAGCGAAGGGCTTCAGAGACACTGAGCGGTACTGGCTCCGAAGAGGACACACCTGCATCCGAGCCAGCCGCTGCACTGCCACGAAAATCTGGCCGAGCCGCTGCAACTAGAAGTAGAATCCCCCGGCCCATTGGACTGCGCATGCCTATGCCAGTGGCCGCTCAGCAGCCTGCTAGCAGATCCCACGGAGCAGCACCAGCACTGGACACTGCCATCACCTCTCGACTGCAGCTCCAGACCCCACCAGGCAGTGCCACAGCTGCAGATCTGAGGCCAAAGCAGCCTCCAGGACGAGGACTCGGTCCAGGAAGGGCACAGGCAGGAGCTAGACCCCCTGCACCTCGGTCCCCACGACTGCCAGCATCTACAAGTGCCGCTAGAAACGCATCAGCCAGCCCACGGTCCCAGTCTCTGAGTAGACGGGAGTCACCCAGCCCTTCCCATCAGGCACGACCAGGAGTGCCACCACCTCGAGGTGTCCCACCCGCTCGAGCACAGCCAGACGGAACCCCATCTCCAGGAGGTAGTAAGAAAGGTCCCAGGGGCAAGCTGCAGGCCCAGAGGGCAACTACTAAAGGTCGGGCAGGAGGTGCTGAAGGAAGGGCAGGGGCAAGGTGA

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMQCLAA
ALKDETNMSGGGEQADILPANYVVKDRWKVLKKIGGGGFGEIYEAMDLLT
RENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVV
MQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIK
PSNFAMGRLPSTYRKCYMLAFGLARQYTNTTGDVRPPRNVAGFRGTVRYA
SVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYE
HRMLLKHMPSEFHLFLDHIASLDYFTKPDYQLIMSVFENSMKERGIAENE
AFDWEKAGTDALLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTE
DVLQGEHLSDQENAPPILPGRPSEGLGPSPHLVPHPGGPEAEVWEETDVN
RNKLRINIGKSPCVEEEQSRGMGVPSSPVRAPPDSPTTPVRSLRYRRVNS
PESERLSTADGRVELPERRSRMDLPGSPSRQACSSQPAQMLSVDTGHADR
QASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFP
PGAEPSTSGTTDEEPEELRPLPEEGEERRRLGAEPTVRPRGRSMQALAEE
DLQHLPPQPLPPQLSQGDGRSETSQPPTPGSPSHSPLHSGPRPRRRESDP
TGPQRQVFSVAPPFEVNGLPRAVPLSLPYQDFKRDLSDYRERARLLNRVR
RVGFSHMLLTTPQVPLAPVQPQANGKEEEEEEEEDEEEEEEDEEEEEEEE
EEEEEEEEEEEEEEEAAAAVALGEVLGPRSGSSSEGSERSTDRSQEGAPS
TLLADDQKESRGRASMADGDLEPEEGSKTLVLVSPGDMKKSPVTAELAPD
PDLGTLAALTPQHERPQPTGSQLDVSEPGTLSSVLKSEPKPPGPGAGLGA
GTVTTGVGGVAVTSSPFTKVERTFVHIAEKTHLNVMSSGGQALRSEEFSA
GGELGLELASDGGAVEEGARAPLENGLALSGLNGAEIEGSALSGAPRETP
SEMATNSLPNGPALADGPAPVSPLEPSPEKVATISPRRHAMPGSRPRSRI
PVLLSEEDTGSEPSGSLSAKERWSKRARPQQDLARLVMEKRQGRLLLRLA
SGASSSSSEEQRRASETLSGTGSEEDTPASEPAAALPRKSGRAAATRSRI
PRPIGLRMPMPVAAQQPASRSHGAAPALDTAITSRLQLQTPPGSATAADL
RPKQPPGRGLGPGRAQAGARPPAPRSPRLPASTSAARNASASPRSQSLSR
RESPSPSHQARPGVPPPRGVPPARAQPDGTPSPGGSKKGPRGKLQAQRAT
TKGRAGGAEGRAGAR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44927
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D176A (KD)