cDNA Clone UBE3C

• Synonyms: HECTH2
• Plasmid: pcDNA5 FRT/TO FLAG UBE3C
• Parent Plasmid: pcDNA5 FRT/TO FLAG
• Expressed: FLAG-UBE3C
• Genbank: NM_014671.2 GI:187960099 with silent change a1686g
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGTTCAGCTTCGAAGGCGACTTCAAGACGCGGCCCAAGGTGTCCCTTGGCGGCGCGAGCAGGAAGGAGGAAAAGGCTTCTCTTTTACATCGTACTCAGGAAGAAAGAAGAAAGAGAGAGGAAGAAAGGCGAAGGTTGAAAAATGCAATAATTATCCAGTCATTTATTCGAGGCTATAGAGACAGAAAACAGCAATATTCCATCCAAAGAAGTGCATTTGATCGCTGTGCTACCTTGTCACAGTCCGGGGGCGCTTTTCCCATTGCTAATGGCCCCAACCTTACCCTTTTGGTAAGGCAGCTTCTGTTTTTTTACAAACAAAATGAAGACTCAAAACGTTTGATATGGCTGTATCAGAACTTAATTAAACACAGCTCTCTGTTTGTCAAGCAGTTGGATGGATCTGAGAGACTTACATGCTTATTTCAGATAAAAAGATTGATGAGCCTCTGTTGCAGGTTGCTGCAAAACTGTAATGATGACAGTTTGAATGTTGCACTTCCAATGAGAATGCTTGAAGTATTTTCGTCTGAGAATACTTACTTGCCTGTTTTACAAGATGCTAGCTATGTGGTGTCAGTGATTGAACAAATTTTGCACTACATGATTCACAATGGGTATTATAGGTCTCTATATTTGTTGATTAACAGCAAGCTTCCATCAAGTATTGAATATTCTGATTTATCTCGAGTTCCTATAGCAAAAATTTTGCTAGAGAATGTTCTAAAACCATTGCACTTTACTTACAACTCCTGTCCGGAAGGTGCGAGGCAACAAGTTTTTACAGCCTTCACAGAGGAGTTTCTGGCAGCACCTTTTACAGATCAGATTTTTCATTTCATCATTCCGGCGCTTGCAGATGCGCAGACCGTTTTCCCTTACGAGCCCTTTCTGAATGCACTGTTGTTAATAGAGAGTAGATGTTCAAGAAAGAGTGGTGGAGCACCCTGGCTTTTCTATTTCGTTTTAACTGTTGGCGAAAATTATTTGGGGGCCCTCTCTGAGGAAGGGCTGCTGGTGTATTTGCGGGTGCTGCAGACCTTCCTCTCTCAGTTACCAGTCTCTCCTGCCAGCGCGAGCTGTCACGACTCAGCCAGTGACTCTGAGGAGGAGAGTGAAGAAGCCGACAAGCCCTCAAGCCCGGAGGATGGCAGACTGTCAGTATCATACATAACAGAGGAATGCCTGAAGAAGCTGGACACAAAGCAGCAGACCAACACCCTGCTCAACCTGGTGTGGAGGGACTCTGCGAGCGAGGAGGTCTTCACCACCATGGCCTCCGTCTGCCACACGCTGATGGTGCAGCACCGCATGATGGTACCCAAAGTCAGGCTTCTCTACAGTTTAGCCTTTAATGCCAGGTTTCTGAGACATCTTTGGTTTCTAATATCTTCCATGTCAACACGGATGATCACAGGGTCTATGGTACCGTTGCTTCAGGTGATATCCAGGGGTTCTCCTATGTCTTTTGAAGATTCTAGTCGAATCATCCCACTCTTTTATCTTTTTAGCTCCTTGTTTAGTCATTCACTAATTTCCATACATGATAACGAATTCTTCGGTGATCCCATAGAAGTTGTAGGTCAAAGACAATCATCAATGATGCCTTTTACTTTAGAAGAGCTGATAATGTTGTCTCGATGCCTTCGAGATGCATGCCTGGGGATCATCAAGTTGGCTTATCCGGAAACCAAGCCAGAAGTTCGAGAAGAATATATTACAGCATTTCAGAGTATTGGAGTTACTACTAGCTCTGAAATGCAACAATGCATACAGATGGAACAGAAAAGATGGATTCAGTTATTTAAGGTTATCACCAATCTAGTGAAAATGTTGAAGTCCAGAGACACGAGGAGAAATTTTTGTCCTCCAAACCACTGGCTGTCAGAACAAGAAGATATTAAAGCAGATAAGGTCACTCAGCTCTATGTGCCAGCATCCAGACATGTGTGGAGGTTCCGGCGGATGGGGAGGATAGGCCCGCTGCAGTCCACCCTGGACGTGGGTTTGGAGTCCCCGCCGCTGTCTGTGTCTGAGGAAAGACAGCTTGCTGTCCTGACAGAGTTGCCTTTTGTGGTTCCATTTGAGGAACGAGTAAAGATCTTTCAGAGGTTGATTTATGCAGATAAGCAAGAAGTTCAAGGAGATGGTCCATTTCTGGATGGAATTAATGTCACAATAAGAAGAAATTACATTTATGAAGATGCTTATGACAAACTTTCTCCAGAAAATGAGCCTGATTTGAAAAAGCGGATCCGTGTGCACTTGCTCAATGCCCATGGCCTGGATGAAGCTGGCATTGATGGTGGTGGTATTTTCAGAGAGTTTTTAAATGAACTACTGAAGTCAGGATTTAACCCCAACCAGGGGTTCTTTAAGACTACTAATGAAGGGCTTCTGTACCCCAACCCGGCTGCTCAGATGCTTGTGGGAGATTCTTTTGCCAGACATTACTACTTCCTAGGCAGAATGCTTGGAAAGGCTCTCTATGAGAACATGCTGGTGGAGCTGCCCTTTGCAGGCTTCTTTCTTTCCAAGTTGCTTGGAACCAGTGCCGACGTGGACATTCACCACCTCGCCTCCCTAGACCCTGAGGTGTATAAGAATTTGCTCTTTCTGAAGAGCTACGAAGACGATGTGGAGGAGCTTGGGCTGAACTTCACTGTGGTGAACAATGACCTGGGAGAGGCGCAGGTAGTTGAACTAAAATTCGGTGGGAAAGACATCCCTGTCACCAGCGCCAACCGGATTGCGTACATCCACTTGGTGGCAGACTACAGGCTGAACAGGCAGATCCGCCAGCACTGCCTGGCTTTCCGCCAGGGCCTTGCCAATGTCGTCAGCCTCGAGTGGCTCCGAATGTTTGATCAGCAAGAAATTCAGGTATTAATTTCTGGTGCACAAGTTCCCATAAGCCTAGAGGACCTAAAATCCTTTACAAACTATTCAGGAGGCTATTCTGCAGACCATCCTGTTATTAAGGTCTTCTGGAGAGTTGTGGAAGGGTTCACTGATGAAGAAAAGCGCAAACTGCTGAAGTTTGTAACAAGCTGCTCTCGACCCCCTCTCTTGGGGTTTAAGGAGTTGTATCCCGCATTTTGTATTCACAACGGAGGCTCCGACCTTGAGCGGCTCCCCACAGCCAGCACCTGCATGAACCTGCTGAAGCTCCCCGAGTTCTATGACGAGACACTTTTGCGAAGTAAACTTCTCTATGCGATTGAATGTGCCGCTGGCTTTGAGCTGAGCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKR
EEERRRLKNAIIIQSFIRGYRDRKQQYSIQRSAFDRCATLSQSGGAFPIA
NGPNLTLLVRQLLFFYKQNEDSKRLIWLYQNLIKHSSLFVKQLDGSERLT
CLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLQDAS
YVVSVIEQILHYMIHNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLE
NVLKPLHFTYNSCPEGARQQVFTAFTEEFLAAPFTDQIFHFIIPALADAQ
TVFPYEPFLNALLLIESRCSRKSGGAPWLFYFVLTVGENYLGALSEEGLL
VYLRVLQTFLSQLPVSPASASCHDSASDSEEESEEADKPSSPEDGRLSVS
YITEECLKKLDTKQQTNTLLNLVWRDSASEEVFTTMASVCHTLMVQHRMM
VPKVRLLYSLAFNARFLRHLWFLISSMSTRMITGSMVPLLQVISRGSPMS
FEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTL
EELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQ
CIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKV
TQLYVPASRHVWRFRRMGRIGPLQSTLDVGLESPPLSVSEERQLAVLTEL
PFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDK
LSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQ
GFFKTTNEGLLYPNPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPF
AGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEDDVEELGLNFTV
VNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAF
RQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAD
HPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGG
SDLERLPTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45917
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee